BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0577100 Os03g0577100|AK101786
         (482 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0577100  Nuf2 family protein                                 948   0.0  
Os08g0163100  Nuf2 family protein                                 873   0.0  

>Os03g0577100 Nuf2 family protein
          Length = 482

 Score =  948 bits (2450), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 470/482 (97%), Positives = 470/482 (97%)

Query: 1   MASNFSFPPLAPEQIAEALHTYGLAPTANLRAEDIANPQPDLLPAVISNFLATVVDPTGA 60
           MASNFSFPPLAPEQIAEALHTYGLAPTANLRAEDIANPQPDLLPAVISNFLATVVDPTGA
Sbjct: 1   MASNFSFPPLAPEQIAEALHTYGLAPTANLRAEDIANPQPDLLPAVISNFLATVVDPTGA 60

Query: 61  DDLDGQLGFDALASLDNPEHYREGIRVLRLHKRANAFLESIQFPGFTXXXXXXXXXXXXV 120
           DDLDGQLGFDALASLDNPEHYREGIRVLRLHKRANAFLESIQFPGFT            V
Sbjct: 61  DDLDGQLGFDALASLDNPEHYREGIRVLRLHKRANAFLESIQFPGFTLRDLLRPDPRRLV 120

Query: 121 QVLSALINFLYYRDEKLALLQPIIHEFPNLDERCMELNAKIAEHQKAIADQELAAQMEVP 180
           QVLSALINFLYYRDEKLALLQPIIHEFPNLDERCMELNAKIAEHQKAIADQELAAQMEVP
Sbjct: 121 QVLSALINFLYYRDEKLALLQPIIHEFPNLDERCMELNAKIAEHQKAIADQELAAQMEVP 180

Query: 181 MVQQLEAEVNSLKQKLVEYNKKQLALRANATAINDKKEETHRKIAKSDFELVKLAQENSK 240
           MVQQLEAEVNSLKQKLVEYNKKQLALRANATAINDKKEETHRKIAKSDFELVKLAQENSK
Sbjct: 181 MVQQLEAEVNSLKQKLVEYNKKQLALRANATAINDKKEETHRKIAKSDFELVKLAQENSK 240

Query: 241 LLSKIVQSPEKLQRALEEKKTARAELKNAEKIAMQSVQEKTATLEIYSKGYEKLSKHSSK 300
           LLSKIVQSPEKLQRALEEKKTARAELKNAEKIAMQSVQEKTATLEIYSKGYEKLSKHSSK
Sbjct: 241 LLSKIVQSPEKLQRALEEKKTARAELKNAEKIAMQSVQEKTATLEIYSKGYEKLSKHSSK 300

Query: 301 IQALQEQFTATKALEKEVKARKAKISDESVEIMALDTKTIEWDGKVHEMEERVKAKEKER 360
           IQALQEQFTATKALEKEVKARKAKISDESVEIMALDTKTIEWDGKVHEMEERVKAKEKER
Sbjct: 301 IQALQEQFTATKALEKEVKARKAKISDESVEIMALDTKTIEWDGKVHEMEERVKAKEKER 360

Query: 361 DQIVADENQKLAALRSEVEWKLKCLEPRERKVEETIAKATKLCAEVDSTRTDAAEELRLI 420
           DQIVADENQKLAALRSEVEWKLKCLEPRERKVEETIAKATKLCAEVDSTRTDAAEELRLI
Sbjct: 361 DQIVADENQKLAALRSEVEWKLKCLEPRERKVEETIAKATKLCAEVDSTRTDAAEELRLI 420

Query: 421 YAKFQQIGHAFTCYKDNFKTFLEQVDEVSKETLESLGRQAVEPLDTSATLSIEKENSCGP 480
           YAKFQQIGHAFTCYKDNFKTFLEQVDEVSKETLESLGRQAVEPLDTSATLSIEKENSCGP
Sbjct: 421 YAKFQQIGHAFTCYKDNFKTFLEQVDEVSKETLESLGRQAVEPLDTSATLSIEKENSCGP 480

Query: 481 AP 482
           AP
Sbjct: 481 AP 482



>Os08g0163100 Nuf2 family protein
          Length = 470

 Score =  873 bits (2255), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 434/470 (92%), Positives = 445/470 (94%)

Query: 1   MASNFSFPPLAPEQIAEALHTYGLAPTANLRAEDIANPQPDLLPAVISNFLATVVDPTGA 60
           MASNFSFPPL PEQIAEALHTYGLAPTANLRAEDIANPQPDLLPAVISNFLATVVDPTGA
Sbjct: 1   MASNFSFPPLTPEQIAEALHTYGLAPTANLRAEDIANPQPDLLPAVISNFLATVVDPTGA 60

Query: 61  DDLDGQLGFDALASLDNPEHYREGIRVLRLHKRANAFLESIQFPGFTXXXXXXXXXXXXV 120
           DDLDGQLGFDALASLDNPEHY EGIRVLRLHKRA+AFLESIQFPGFT            V
Sbjct: 61  DDLDGQLGFDALASLDNPEHYMEGIRVLRLHKRASAFLESIQFPGFTLRDLLRPDPRRLV 120

Query: 121 QVLSALINFLYYRDEKLALLQPIIHEFPNLDERCMELNAKIAEHQKAIADQELAAQMEVP 180
           QVLSALINFLYYRD+KLALLQPIIHEFPNLDERCMEL AK+AEHQKAIAD ELAAQME P
Sbjct: 121 QVLSALINFLYYRDDKLALLQPIIHEFPNLDERCMELKAKLAEHQKAIADHELAAQMEEP 180

Query: 181 MVQQLEAEVNSLKQKLVEYNKKQLALRANATAINDKKEETHRKIAKSDFELVKLAQENSK 240
           MVQQ+EAEVNSLKQKLVEYNKKQLALRANATAINDKKEETHRKIAK+DFELVKLAQENSK
Sbjct: 181 MVQQIEAEVNSLKQKLVEYNKKQLALRANATAINDKKEETHRKIAKNDFELVKLAQENSK 240

Query: 241 LLSKIVQSPEKLQRALEEKKTARAELKNAEKIAMQSVQEKTATLEIYSKGYEKLSKHSSK 300
           LL KIV+SPEKLQRALEEKKTARAELKNAEKIA QSVQEKTATLEIYSKGYEKLSKHS+K
Sbjct: 241 LLPKIVESPEKLQRALEEKKTARAELKNAEKIATQSVQEKTATLEIYSKGYEKLSKHSTK 300

Query: 301 IQALQEQFTATKALEKEVKARKAKISDESVEIMALDTKTIEWDGKVHEMEERVKAKEKER 360
           IQALQEQ TATKALEKEVKARK KISDESVEIMALDTK IEWDGKVHEMEE VKAKEK++
Sbjct: 301 IQALQEQVTATKALEKEVKARKTKISDESVEIMALDTKIIEWDGKVHEMEEHVKAKEKKK 360

Query: 361 DQIVADENQKLAALRSEVEWKLKCLEPRERKVEETIAKATKLCAEVDSTRTDAAEELRLI 420
           DQIVADENQKLAAL SEVEWKLKCLEPRERKVEETIAKATKLCAEVDSTRTDAAEEL+LI
Sbjct: 361 DQIVADENQKLAALSSEVEWKLKCLEPRERKVEETIAKATKLCAEVDSTRTDAAEELQLI 420

Query: 421 YAKFQQIGHAFTCYKDNFKTFLEQVDEVSKETLESLGRQAVEPLDTSATL 470
           YAKFQQIGHAFTCYKDNFK+FLEQVDEVSKETLESL RQAVEPLDTSATL
Sbjct: 421 YAKFQQIGHAFTCYKDNFKSFLEQVDEVSKETLESLDRQAVEPLDTSATL 470



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.127    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 13,353,220
Number of extensions: 496222
Number of successful extensions: 2330
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 2337
Number of HSP's successfully gapped: 2
Length of query: 482
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 377
Effective length of database: 11,553,331
Effective search space: 4355605787
Effective search space used: 4355605787
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 158 (65.5 bits)