BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0299600 Os03g0299600|AK068151
         (228 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0299600  Wound-induced WI12 family protein                   352   2e-97
Os05g0342100  Wound-induced WI12 family protein                    83   2e-16

>Os03g0299600 Wound-induced WI12 family protein
          Length = 228

 Score =  352 bits (902), Expect = 2e-97,   Method: Compositional matrix adjust.
 Identities = 181/228 (79%), Positives = 181/228 (79%)

Query: 1   SRARFLDFVSNTGRSGGRSEPKRGSRQELANHRQGTXXXXXXXXXXXXXXXXLDGARDQP 60
           SRARFLDFVSNTGRSGGRSEPKRGSRQELANHRQGT                LDGARDQP
Sbjct: 1   SRARFLDFVSNTGRSGGRSEPKRGSRQELANHRQGTAAAAREASASVSELAELDGARDQP 60

Query: 61  EEQDAKFLVLRLYEALNSGDARRAQELLAPDLEWWFHGPPAHQHMMRLLTGADHGESRFL 120
           EEQDAKFLVLRLYEALNSGDARRAQELLAPDLEWWFHGPPAHQHMMRLLTGADHGESRFL
Sbjct: 61  EEQDAKFLVLRLYEALNSGDARRAQELLAPDLEWWFHGPPAHQHMMRLLTGADHGESRFL 120

Query: 121 FSPRSVDAFGSTVIAEGTDDTRQLYWVHAWTVGPDGVITQLREYFNTDLTVTRLXXXXXX 180
           FSPRSVDAFGSTVIAEGTDDTRQLYWVHAWTVGPDGVITQLREYFNTDLTVTRL      
Sbjct: 121 FSPRSVDAFGSTVIAEGTDDTRQLYWVHAWTVGPDGVITQLREYFNTDLTVTRLSASAAK 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXKCLWQSRRADRAHKSLPGLVLAI 228
                                    KCLWQSRRADRAHKSLPGLVLAI
Sbjct: 181 TTAAISSSNSNHASSSAPPPPPSKPKCLWQSRRADRAHKSLPGLVLAI 228



>Os05g0342100 Wound-induced WI12 family protein
          Length = 177

 Score = 83.2 bits (204), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 55/109 (50%), Positives = 66/109 (60%), Gaps = 7/109 (6%)

Query: 68  LVLRLYEALNSGDARRAQELLAPDLEWWFHGPPAHQHMMRLLTGADHG--ESRFLFSPRS 125
           LV  LY A+ +GD   A  +LA D++WWFHGP   +HM R LTG       S F+F PR 
Sbjct: 21  LVESLYAAVAAGDGAAASAVLAGDVDWWFHGPRRCEHMRRRLTGEAEAASASSFVFVPRQ 80

Query: 126 VDAF---GSTVIAEGTDDTRQLYWVHAWTVGPDGVITQLREYFNTDLTV 171
           V A    G  V+AEG +  R  YWVHAW V   G IT+LREYFNT +TV
Sbjct: 81  VAAVGRGGGWVVAEGWEGPRA-YWVHAWAV-EGGRITRLREYFNTSVTV 127



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.135    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,958,565
Number of extensions: 241450
Number of successful extensions: 636
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 631
Number of HSP's successfully gapped: 3
Length of query: 228
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 131
Effective length of database: 11,971,043
Effective search space: 1568206633
Effective search space used: 1568206633
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 154 (63.9 bits)