BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0296600 Os03g0296600|AK109176
         (151 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0296600  Similar to ECA1 protein                             229   5e-61
Os11g0168000  Protein of unknown function DUF1278 family pro...    94   4e-20
Os12g0167601                                                       86   1e-17

>Os03g0296600 Similar to ECA1 protein
          Length = 151

 Score =  229 bits (584), Expect = 5e-61,   Method: Compositional matrix adjust.
 Identities = 129/151 (85%), Positives = 129/151 (85%)

Query: 1   MACSGSFLPIMXXXXXXXXXXXXXXXXXXXXXXQRLADGVGQQQQQCWEVLMEIKSCTGE 60
           MACSGSFLPIM                      QRLADGVGQQQQQCWEVLMEIKSCTGE
Sbjct: 1   MACSGSFLPIMLLPLLLAGAAVAGGAPPGLGLAQRLADGVGQQQQQCWEVLMEIKSCTGE 60

Query: 61  ILLFFINGEAYLGPGCCRAIRVIEQSCWATDAMLSVIGFTPEEGDMLKGYCDAGDEHKPS 120
           ILLFFINGEAYLGPGCCRAIRVIEQSCWATDAMLSVIGFTPEEGDMLKGYCDAGDEHKPS
Sbjct: 61  ILLFFINGEAYLGPGCCRAIRVIEQSCWATDAMLSVIGFTPEEGDMLKGYCDAGDEHKPS 120

Query: 121 PPPASPAVGYVAVGENAAVPAGRKSLALQHR 151
           PPPASPAVGYVAVGENAAVPAGRKSLALQHR
Sbjct: 121 PPPASPAVGYVAVGENAAVPAGRKSLALQHR 151



>Os11g0168000 Protein of unknown function DUF1278 family protein
          Length = 144

 Score = 94.0 bits (232), Expect = 4e-20,   Method: Compositional matrix adjust.
 Identities = 42/79 (53%), Positives = 57/79 (72%), Gaps = 4/79 (5%)

Query: 47  CWEVLMEIKSCTGEILLFFINGEAYLGPGCCRAIRVIEQSCWATDAMLSVIGFTPEEGDM 106
           CW  + ++ SCT EI+LFF+NGE+YLGP CC AIR + + CW   AML+ IGFT +E D+
Sbjct: 56  CWSAVTKLGSCTNEIVLFFVNGESYLGPDCCVAIRTVTRRCWP--AMLASIGFTAQEADI 113

Query: 107 LKGYCDAGDEHKPSPPPAS 125
           L+G+CDA  E    PPP++
Sbjct: 114 LRGFCDA--ELAAPPPPST 130



>Os12g0167601 
          Length = 139

 Score = 85.9 bits (211), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 42/86 (48%), Positives = 55/86 (63%), Gaps = 9/86 (10%)

Query: 46  QCWEVLMEIKSCTGEILLFFINGEAY-LGPGCCRAIRVIEQSCWATDAMLSVIGFTPEEG 104
           +CW  + E++SCT EI+LFF+NGE   LG GCCRA+R   + CW   AML+ +GFT EE 
Sbjct: 56  ECWSAVAELRSCTDEIVLFFLNGETTQLGAGCCRAVRAATRDCWP--AMLAAVGFTAEEA 113

Query: 105 DMLKGYCD------AGDEHKPSPPPA 124
           D+L+G CD      A D   P+P  A
Sbjct: 114 DVLRGLCDAEAAAAAADSTSPAPSAA 139



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.137    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,379,249
Number of extensions: 235306
Number of successful extensions: 1354
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1350
Number of HSP's successfully gapped: 3
Length of query: 151
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 59
Effective length of database: 12,232,113
Effective search space: 721694667
Effective search space used: 721694667
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 151 (62.8 bits)