BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0291500 Os03g0291500|015-004-C07
         (89 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0291500  Asparagine synthase domain containing protein       133   4e-32
Os06g0265000  Similar to Asparagine synthetase                    108   1e-24

>Os03g0291500 Asparagine synthase domain containing protein
          Length = 89

 Score =  133 bits (334), Expect = 4e-32,   Method: Compositional matrix adjust.
 Identities = 74/89 (83%), Positives = 74/89 (83%)

Query: 1  DGIDAIEEVIYHDETYDVTTIRASTPMFLMARKIKALGVKMVXXXXXXXXXXXXXXXFHF 60
          DGIDAIEEVIYHDETYDVTTIRASTPMFLMARKIKALGVKMV               FHF
Sbjct: 1  DGIDAIEEVIYHDETYDVTTIRASTPMFLMARKIKALGVKMVLSGEGSDELLGGYLYFHF 60

Query: 61 APNKEEFHKETCRKVATADTTTATNRSCC 89
          APNKEEFHKETCRKVATADTTTATNRSCC
Sbjct: 61 APNKEEFHKETCRKVATADTTTATNRSCC 89



>Os06g0265000 Similar to Asparagine synthetase
          Length = 591

 Score =  108 bits (269), Expect = 1e-24,   Method: Composition-based stats.
 Identities = 51/75 (68%), Positives = 58/75 (77%)

Query: 1   DGIDAIEEVIYHDETYDVTTIRASTPMFLMARKIKALGVKMVXXXXXXXXXXXXXXXFHF 60
           +GIDA+EEVIYH ETYDVTTIRASTPMFLM+RKIK+LGVKMV               FH 
Sbjct: 298 EGIDALEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHK 357

Query: 61  APNKEEFHKETCRKV 75
           APNK+EFH+ETCRK+
Sbjct: 358 APNKKEFHEETCRKI 372



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.131    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,032,762
Number of extensions: 39965
Number of successful extensions: 90
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 90
Number of HSP's successfully gapped: 2
Length of query: 89
Length of database: 17,035,801
Length adjustment: 59
Effective length of query: 30
Effective length of database: 13,955,175
Effective search space: 418655250
Effective search space used: 418655250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 149 (62.0 bits)