BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0262900 Os03g0262900|AK070551
         (371 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0262900  Vacuolar sorting protein 9 domain containing p...   756   0.0  
Os03g0842700  Vacuolar sorting protein 9 domain containing p...   202   3e-52

>Os03g0262900 Vacuolar sorting protein 9 domain containing protein
          Length = 371

 Score =  756 bits (1951), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 371/371 (100%), Positives = 371/371 (100%)

Query: 1   MTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIKPEYQSETSWLLAQKELQKI 60
           MTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIKPEYQSETSWLLAQKELQKI
Sbjct: 1   MTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIKPEYQSETSWLLAQKELQKI 60

Query: 61  NMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEFLPVLIYVTIKRYRRQSRLV 120
           NMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEFLPVLIYVTIKRYRRQSRLV
Sbjct: 61  NMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEFLPVLIYVTIKRYRRQSRLV 120

Query: 121 SEAQYFFTNILSAESFIWNIDGESLSMDERDFQKKMDLARERMLGLSASSENQDNQNNLD 180
           SEAQYFFTNILSAESFIWNIDGESLSMDERDFQKKMDLARERMLGLSASSENQDNQNNLD
Sbjct: 121 SEAQYFFTNILSAESFIWNIDGESLSMDERDFQKKMDLARERMLGLSASSENQDNQNNLD 180

Query: 181 VREQKSQTLKASRDSDVNLSLKDNFQGPGLEMRRDSDASSNPVERVQSISDLEKKGAAEL 240
           VREQKSQTLKASRDSDVNLSLKDNFQGPGLEMRRDSDASSNPVERVQSISDLEKKGAAEL
Sbjct: 181 VREQKSQTLKASRDSDVNLSLKDNFQGPGLEMRRDSDASSNPVERVQSISDLEKKGAAEL 240

Query: 241 LKDDDLNKKIQEYPFLFARSGDLTVADVENLLNSYKQLVLKYVALSQGMGINLENPPVQS 300
           LKDDDLNKKIQEYPFLFARSGDLTVADVENLLNSYKQLVLKYVALSQGMGINLENPPVQS
Sbjct: 241 LKDDDLNKKIQEYPFLFARSGDLTVADVENLLNSYKQLVLKYVALSQGMGINLENPPVQS 300

Query: 301 MQTVSDLVESEEPKNVKNAVNFSEGSSKTSDDIKNDTLYSEVDNTGTQQTAVDPSYQKAQ 360
           MQTVSDLVESEEPKNVKNAVNFSEGSSKTSDDIKNDTLYSEVDNTGTQQTAVDPSYQKAQ
Sbjct: 301 MQTVSDLVESEEPKNVKNAVNFSEGSSKTSDDIKNDTLYSEVDNTGTQQTAVDPSYQKAQ 360

Query: 361 QDEASDQPEHA 371
           QDEASDQPEHA
Sbjct: 361 QDEASDQPEHA 371



>Os03g0842700 Vacuolar sorting protein 9 domain containing protein
          Length = 218

 Score =  202 bits (514), Expect = 3e-52,   Method: Compositional matrix adjust.
 Identities = 94/174 (54%), Positives = 127/174 (72%), Gaps = 14/174 (8%)

Query: 1   MTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIKPEYQSETSWLLAQKELQKI 60
           MTKLF+R FAS  EDVKSD E+ EK+ LLQ F+RP +LDI     +E +WLLA KELQKI
Sbjct: 1   MTKLFDRAFASSAEDVKSDMEISEKIGLLQHFVRPHHLDIPKLLHNEAAWLLAVKELQKI 60

Query: 61  NMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEFLPVLIYVTIKR-------- 112
           N +K+PR+KL+CI++CC+VINNLLLN S+ ++    GAD+FLP+LIY+TIK         
Sbjct: 61  NSFKSPREKLSCIMSCCQVINNLLLNVSMSNDRTLSGADDFLPILIYITIKANPPQLHSN 120

Query: 113 ------YRRQSRLVSEAQYFFTNILSAESFIWNIDGESLSMDERDFQKKMDLAR 160
                 +RR++RL+SE +Y+ TN++SA+ FI N++G SLSM+E  FQ  M+ AR
Sbjct: 121 LKFIQLFRRETRLISEVEYYLTNLISAKMFIMNVNGHSLSMEESVFQTHMESAR 174



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.311    0.128    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,334,093
Number of extensions: 436106
Number of successful extensions: 1034
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1033
Number of HSP's successfully gapped: 2
Length of query: 371
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 269
Effective length of database: 11,709,973
Effective search space: 3149982737
Effective search space used: 3149982737
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 157 (65.1 bits)