BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0206400 Os03g0206400|AK066494
(91 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os03g0206400 Conserved hypothetical protein 152 5e-38
Os03g0762500 Conserved hypothetical protein 69 6e-13
Os07g0115500 Conserved hypothetical protein 65 7e-12
>Os03g0206400 Conserved hypothetical protein
Length = 91
Score = 152 bits (384), Expect = 5e-38, Method: Compositional matrix adjust.
Identities = 77/91 (84%), Positives = 77/91 (84%)
Query: 1 MGYWWDRVVLPVRRAWLGVAARFGVRQTGLWRLRQEVSTCEYEDVRVMWEMLSRTSTXXX 60
MGYWWDRVVLPVRRAWLGVAARFGVRQTGLWRLRQEVSTCEYEDVRVMWEMLSRTST
Sbjct: 1 MGYWWDRVVLPVRRAWLGVAARFGVRQTGLWRLRQEVSTCEYEDVRVMWEMLSRTSTAGA 60
Query: 61 XXXXXXXXXXXHSRFRQPRPWTESICLCAGF 91
HSRFRQPRPWTESICLCAGF
Sbjct: 61 GAGRAAPPARRHSRFRQPRPWTESICLCAGF 91
>Os03g0762500 Conserved hypothetical protein
Length = 94
Score = 69.3 bits (168), Expect = 6e-13, Method: Compositional matrix adjust.
Identities = 31/53 (58%), Positives = 41/53 (77%), Gaps = 1/53 (1%)
Query: 6 DRVVLPVRRAWLGVAARFGVRQT-GLWRLRQEVSTCEYEDVRVMWEMLSRTST 57
DR +PV+R WLG+AAR G+R+T GL +L++EV TCEY DV +MWEML +T
Sbjct: 4 DRAAVPVKRVWLGLAARLGLRRTSGLGKLKKEVRTCEYHDVHIMWEMLRKTDA 56
>Os07g0115500 Conserved hypothetical protein
Length = 94
Score = 65.5 bits (158), Expect = 7e-12, Method: Compositional matrix adjust.
Identities = 32/54 (59%), Positives = 39/54 (72%), Gaps = 1/54 (1%)
Query: 1 MGYWWDRVVLPVRRAWLGVAARFGVRQT-GLWRLRQEVSTCEYEDVRVMWEMLS 53
M R +PV+R WLG++AR G+R+T GL +LR EV TCEY DV VMWEMLS
Sbjct: 1 MSCLLGRAAVPVKRVWLGLSARLGLRRTTGLGKLRNEVRTCEYSDVHVMWEMLS 54
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.329 0.137 0.506
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,803,541
Number of extensions: 75078
Number of successful extensions: 254
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 254
Number of HSP's successfully gapped: 3
Length of query: 91
Length of database: 17,035,801
Length adjustment: 61
Effective length of query: 30
Effective length of database: 13,850,747
Effective search space: 415522410
Effective search space used: 415522410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 149 (62.0 bits)