BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0194100 Os03g0194100|AK073045
         (402 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0194100  C2 calcium/lipid-binding region, CaLB domain c...   826   0.0  
Os07g0585000  C2 calcium/lipid-binding region, CaLB domain c...   291   7e-79
Os01g0953500  C2 calcium/lipid-binding region, CaLB domain c...   203   2e-52

>Os03g0194100 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 402

 Score =  826 bits (2134), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 402/402 (100%), Positives = 402/402 (100%)

Query: 1   MAAYQTGPVSEPPMLNTEIITQKVTGFNTETTTKEKPIGYLDVFVHQARDIHNICIYHKQ 60
           MAAYQTGPVSEPPMLNTEIITQKVTGFNTETTTKEKPIGYLDVFVHQARDIHNICIYHKQ
Sbjct: 1   MAAYQTGPVSEPPMLNTEIITQKVTGFNTETTTKEKPIGYLDVFVHQARDIHNICIYHKQ 60

Query: 61  DVYAKLCLTSDPDVSCSTKVINGGGRNPVFDDGLRLDVRTVDASLKCEIWMLSRVRNYLE 120
           DVYAKLCLTSDPDVSCSTKVINGGGRNPVFDDGLRLDVRTVDASLKCEIWMLSRVRNYLE
Sbjct: 61  DVYAKLCLTSDPDVSCSTKVINGGGRNPVFDDGLRLDVRTVDASLKCEIWMLSRVRNYLE 120

Query: 121 DQLLGFALVPLADIVMADGKLVQEFSMTSTDLLHTPAGFVQLSLSYVGCSPDVIPIPAPN 180
           DQLLGFALVPLADIVMADGKLVQEFSMTSTDLLHTPAGFVQLSLSYVGCSPDVIPIPAPN
Sbjct: 121 DQLLGFALVPLADIVMADGKLVQEFSMTSTDLLHTPAGFVQLSLSYVGCSPDVIPIPAPN 180

Query: 181 KSALVVNGSGNDSSVPCELEKIEFPDLNVVNENQIMVSKYFEMETLSYEDSVKVDNPKLV 240
           KSALVVNGSGNDSSVPCELEKIEFPDLNVVNENQIMVSKYFEMETLSYEDSVKVDNPKLV
Sbjct: 181 KSALVVNGSGNDSSVPCELEKIEFPDLNVVNENQIMVSKYFEMETLSYEDSVKVDNPKLV 240

Query: 241 QSDAAVPGTELFNKNLDEYREGSPQSCVSTTDYSTATSVTPHSVSEPSDTILAASPTGSQ 300
           QSDAAVPGTELFNKNLDEYREGSPQSCVSTTDYSTATSVTPHSVSEPSDTILAASPTGSQ
Sbjct: 241 QSDAAVPGTELFNKNLDEYREGSPQSCVSTTDYSTATSVTPHSVSEPSDTILAASPTGSQ 300

Query: 301 REKSQDVTDGEADSSDVPLKGEVVKPVISINLNPGESVVQEDIVNMYMKSMQQFTESLAK 360
           REKSQDVTDGEADSSDVPLKGEVVKPVISINLNPGESVVQEDIVNMYMKSMQQFTESLAK
Sbjct: 301 REKSQDVTDGEADSSDVPLKGEVVKPVISINLNPGESVVQEDIVNMYMKSMQQFTESLAK 360

Query: 361 MKLPLDVENSSPSTENNTDSITAEKPSPSKGSRVFYGSRAFF 402
           MKLPLDVENSSPSTENNTDSITAEKPSPSKGSRVFYGSRAFF
Sbjct: 361 MKLPLDVENSSPSTENNTDSITAEKPSPSKGSRVFYGSRAFF 402



>Os07g0585000 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 445

 Score =  291 bits (744), Expect = 7e-79,   Method: Compositional matrix adjust.
 Identities = 187/427 (43%), Positives = 238/427 (55%), Gaps = 69/427 (16%)

Query: 38  IGYLDVFVHQARDIHNICIYHKQDVYAKLCLTSDPDVSCSTKVINGGGRNPVFDDGLRLD 97
           IGY+DV V  ARDI NICIYHKQDVYA+L L  +   + ST+VINGGGRNPVFD  LRL 
Sbjct: 26  IGYVDVHVQSARDIQNICIYHKQDVYARLSLPGEGAPAASTQVINGGGRNPVFDQSLRLG 85

Query: 98  VRT--VDASLKCEIWMLSRVRNYLEDQLLGFALVPLADIVMAD-GKLVQEFSMTSTDLLH 154
           VR   VD +L+CE+WMLSRV+NYL+DQLLGFALVPL D+V A+ G L +EF +++ D+ H
Sbjct: 86  VRAGDVDGALRCEVWMLSRVKNYLQDQLLGFALVPLPDVVAAEGGTLAREFPLSTNDIFH 145

Query: 155 TPAGFVQLSLSYVGCSPDVIPIPAPNKSALV------VNGSGNDSSVPCELEKIEFPDLN 208
           + AGF+QL LSY+G  P+V+PI    K AL         G+ N      E EKIEFPDLN
Sbjct: 146 SHAGFLQLELSYIGVVPEVVPISPTPKPALADAEEHRAGGAANGK----EYEKIEFPDLN 201

Query: 209 VVNENQIMVSKYFEM----------ETL--SYEDSVKVDNPKLVQSDAAVPGTELFNKNL 256
           +V ENQIM+S+Y  +          E+L  S ED         V     V      N   
Sbjct: 202 LVEENQIMLSEYIGLPCSAVEPQSSESLLTSEEDGDGATAETHVAGVRVVQSFSTDNSTA 261

Query: 257 DE---YREGSPQSCVSTTDYSTATSVTPHSVSEPSDTILAASPTGSQREK-SQDVTDGEA 312
           D    +R  +P S VSTT+   A +V     S  S   ++++    Q+EK + D  D E 
Sbjct: 262 DSAGTFRSDTPVSSVSTTESPAAAAVPATPQSNSSGNAVSSA---EQKEKAASDAADAEV 318

Query: 313 DSSDV-------------PLKGEVVK--PVISINLNPGESVVQEDIVNMYMKSMQQFTES 357
           +SS                +    V   PVI +NL     V Q++I++MYMKSMQQFTES
Sbjct: 319 ESSRTVQSSASPAANSPGAMSESAVDKPPVIRVNLEQEVKVDQKEIMDMYMKSMQQFTES 378

Query: 358 LAKMKLPLDVENSS-------------------PSTENNTDSITAEKP---SPSKGSRVF 395
           LAKMKLPLD++N S                    +      +  A+KP    P K  +VF
Sbjct: 379 LAKMKLPLDLDNGSDKSAAVSGAGAAASDADSSGADSGAAAAAAAKKPMAGGPEKSPKVF 438

Query: 396 YGSRAFF 402
           YGSRAFF
Sbjct: 439 YGSRAFF 445



>Os01g0953500 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 397

 Score =  203 bits (516), Expect = 2e-52,   Method: Compositional matrix adjust.
 Identities = 151/382 (39%), Positives = 205/382 (53%), Gaps = 30/382 (7%)

Query: 40  YLDVFVHQARDIHNICIYHKQDVYAKLCLTSDPD--VSCSTKVINGGGRNPVFDDGL-RL 96
           +LD++VH AR IHNICIY  QDVYA+L LTS PD   +  T+V  GGG NP FD+ L  L
Sbjct: 27  FLDIYVHGARGIHNICIYAAQDVYARLALTSSPDDAPALDTRVAAGGGANPRFDERLPPL 86

Query: 97  DVRTVDAS---LKCEIWMLSRVRNYLEDQLLGF-ALVPLADIVMADGKLVQEFSMTSTDL 152
            VR        LKCEIWM S  R  L+DQLLGF  +   A       +L Q+FS++STDL
Sbjct: 87  RVRRARLGTDVLKCEIWMRSCARRLLDDQLLGFALVPLAAVAAADGARLAQDFSLSSTDL 146

Query: 153 LHTPAGFVQLSLS-YVGCSPDVIPIP--APNKSALVVNGSGNDSSVPCELEKIEFPDLNV 209
            H+PAG ++LSL+ + G   D  P P  A  + ++       + + P +  +IEFPDLN 
Sbjct: 147 FHSPAGTIRLSLALHSGPPGDACPPPERAAAEPSITSEVVILEPAPPVDYARIEFPDLNA 206

Query: 210 VNENQIMVSKYFEMETLSYEDSVKVDNPKLVQSDAAVPGTELFNKNLDEYREGSPQSCVS 269
           V EN  M  +Y     L+   ++  D+ ++  S    P  E    + D    GS  +  S
Sbjct: 207 VKENDDMAVQYLPFLHLADAQAMDDDDCEMATS----PREEKPASSDD----GSKNASTS 258

Query: 270 TTDYSTATSVTPHSVSEPS--DTILAASPTGSQREKSQDVTDGEADSSDVPLKGEV---- 323
           TT   TA S    +V+  +  D   AA+   S R      ++G   SS    KG      
Sbjct: 259 TT---TAVSDDNRAVTRKARPDADDAATAPMSCRSPDTPTSNGGKPSSSDKEKGAAEDVF 315

Query: 324 -VKPVISINLNPGESVVQEDIVNMYMKSMQQFTESLAKMKLPLDVENSSPSTENNTDSIT 382
              P+  I++   +S +Q  I+ MYMKSMQQFTESL+KMKLP++++  +       +   
Sbjct: 316 DSPPLRDIDMEAEQSAMQRQIMEMYMKSMQQFTESLSKMKLPMELDGDNGVVVQKEEKPE 375

Query: 383 AE-KPSPSK-GSRVFYGSRAFF 402
           AE K  P K G+RVFYGSRAFF
Sbjct: 376 AEVKLQPKKDGARVFYGSRAFF 397



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.130    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 14,006,882
Number of extensions: 599323
Number of successful extensions: 1513
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1502
Number of HSP's successfully gapped: 3
Length of query: 402
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 299
Effective length of database: 11,657,759
Effective search space: 3485669941
Effective search space used: 3485669941
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 157 (65.1 bits)