BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0128200 Os03g0128200|J065104E23
(55 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os03g0128200 Conserved hypothetical protein 117 3e-27
Os02g0832400 Similar to Callose synthase 1 catalytic subunit 93 4e-20
Os01g0533800 Glycosyl transferase, family 48 protein 73 6e-14
Os01g0533000 69 9e-13
Os01g0754200 Glycosyl transferase, family 48 protein 63 5e-11
>Os03g0128200 Conserved hypothetical protein
Length = 55
Score = 117 bits (292), Expect = 3e-27, Method: Compositional matrix adjust.
Identities = 55/55 (100%), Positives = 55/55 (100%)
Query: 1 ASIIFLNDRLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECICYIYHHVWCE 55
ASIIFLNDRLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECICYIYHHVWCE
Sbjct: 1 ASIIFLNDRLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECICYIYHHVWCE 55
>Os02g0832400 Similar to Callose synthase 1 catalytic subunit
Length = 815
Score = 93.2 bits (230), Expect = 4e-20, Method: Compositional matrix adjust.
Identities = 42/46 (91%), Positives = 44/46 (95%)
Query: 10 LPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECICYIYHHVWCE 55
LPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPEC+CYIYHH+ E
Sbjct: 329 LPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYIYHHMAFE 374
>Os01g0533800 Glycosyl transferase, family 48 protein
Length = 793
Score = 72.8 bits (177), Expect = 6e-14, Method: Composition-based stats.
Identities = 28/41 (68%), Positives = 36/41 (87%)
Query: 12 TIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECICYIYHHV 52
T+ Q QQ LL++GLYLLIWGEA+N+RFMPEC+CYI+HH+
Sbjct: 344 TVTQNKQQLMLLHIGLYLLIWGEASNVRFMPECLCYIFHHM 384
>Os01g0533000
Length = 1224
Score = 68.6 bits (166), Expect = 9e-13, Method: Compositional matrix adjust.
Identities = 29/45 (64%), Positives = 37/45 (82%), Gaps = 1/45 (2%)
Query: 7 NDRLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECICYIYHH 51
N ++P QQ ++LY+GLYLLIWGEA+N+RFMPECICYI+HH
Sbjct: 179 NIKIPN-DASTQQPEILYIGLYLLIWGEASNVRFMPECICYIFHH 222
>Os01g0754200 Glycosyl transferase, family 48 protein
Length = 1790
Score = 62.8 bits (151), Expect = 5e-11, Method: Composition-based stats.
Identities = 25/31 (80%), Positives = 29/31 (93%)
Query: 22 LLYMGLYLLIWGEAANLRFMPECICYIYHHV 52
LLY LYLLIWGEAANLRFMPEC+CYI+H++
Sbjct: 175 LLYTALYLLIWGEAANLRFMPECLCYIFHYM 205
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.333 0.145 0.502
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,717,580
Number of extensions: 44204
Number of successful extensions: 137
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 137
Number of HSP's successfully gapped: 6
Length of query: 55
Length of database: 17,035,801
Length adjustment: 28
Effective length of query: 27
Effective length of database: 15,573,809
Effective search space: 420492843
Effective search space used: 420492843
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 149 (62.0 bits)