BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0109800 Os03g0109800|AK066015
(218 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os03g0109800 Protein of unknown function DUF827, plant fami... 412 e-115
Os09g0458000 Protein of unknown function DUF827, plant fami... 67 8e-12
>Os03g0109800 Protein of unknown function DUF827, plant family protein
Length = 218
Score = 412 bits (1058), Expect = e-115, Method: Compositional matrix adjust.
Identities = 200/218 (91%), Positives = 200/218 (91%)
Query: 1 QYVAVRYIFSFVLHLCCGMDEAPAPLGSSASHQESEGDQCQLQXXXXXXXXXXXXXXXXX 60
QYVAVRYIFSFVLHLCCGMDEAPAPLGSSASHQESEGDQCQLQ
Sbjct: 1 QYVAVRYIFSFVLHLCCGMDEAPAPLGSSASHQESEGDQCQLQADRSHASASNDSSSKAS 60
Query: 61 XQMANRSVQTRIDTTAPIDSVKGAANKFGGSLDLRERRKQAQDELDKVQEKVSECLKRSQ 120
QMANRSVQTRIDTTAPIDSVKGAANKFGGSLDLRERRKQAQDELDKVQEKVSECLKRSQ
Sbjct: 61 DQMANRSVQTRIDTTAPIDSVKGAANKFGGSLDLRERRKQAQDELDKVQEKVSECLKRSQ 120
Query: 121 EAEAGRAQAVKELGGANGVIDELTLGLEMASPRTGLAEISVFDGGEGRGNPHILSPRGGY 180
EAEAGRAQAVKELGGANGVIDELTLGLEMASPRTGLAEISVFDGGEGRGNPHILSPRGGY
Sbjct: 121 EAEAGRAQAVKELGGANGVIDELTLGLEMASPRTGLAEISVFDGGEGRGNPHILSPRGGY 180
Query: 181 MPRTEMAAAEVEAGAKQRPTFFPRMVMFLARKRAQNWK 218
MPRTEMAAAEVEAGAKQRPTFFPRMVMFLARKRAQNWK
Sbjct: 181 MPRTEMAAAEVEAGAKQRPTFFPRMVMFLARKRAQNWK 218
>Os09g0458000 Protein of unknown function DUF827, plant family protein
Length = 835
Score = 67.4 bits (163), Expect = 8e-12, Method: Composition-based stats.
Identities = 39/98 (39%), Positives = 58/98 (59%), Gaps = 5/98 (5%)
Query: 62 QMANRSVQT-----RIDTTAPIDSVKGAANKFGGSLDLRERRKQAQDELDKVQEKVSECL 116
++A R +QT +DT API+SVK A +KFGG LD +ERRK Q ELDK+QE E
Sbjct: 196 EIAERFIQTIDKRVLVDTAAPIESVKDAVSKFGGILDWKERRKHVQVELDKMQEDAPEYK 255
Query: 117 KRSQEAEAGRAQAVKELGGANGVIDELTLGLEMASPRT 154
+R + E +++ ++EL I+ L + L+ + T
Sbjct: 256 RRVEVTEVEKSKVLEELYCTRRTIERLKIDLDKSHTET 293
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.316 0.131 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,112,414
Number of extensions: 298643
Number of successful extensions: 1188
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1189
Number of HSP's successfully gapped: 2
Length of query: 218
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 121
Effective length of database: 11,971,043
Effective search space: 1448496203
Effective search space used: 1448496203
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 154 (63.9 bits)