BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0728300 Os02g0728300|J065025F11
         (118 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0728300  Longevity-assurance protein (LAG1) domain cont...   240   1e-64
Os02g0581300  TRAM, LAG1 and CLN8 homology domain containing...   170   2e-43

>Os02g0728300 Longevity-assurance protein (LAG1) domain containing protein
          Length = 118

 Score =  240 bits (613), Expect = 1e-64,   Method: Compositional matrix adjust.
 Identities = 118/118 (100%), Positives = 118/118 (100%)

Query: 1   MGVPPVDWEAESYPAYSDFAAIPLFAVFLFAVRYLLDRFVFEWLARRLIFEKDEKLDLAT 60
           MGVPPVDWEAESYPAYSDFAAIPLFAVFLFAVRYLLDRFVFEWLARRLIFEKDEKLDLAT
Sbjct: 1   MGVPPVDWEAESYPAYSDFAAIPLFAVFLFAVRYLLDRFVFEWLARRLIFEKDEKLDLAT 60

Query: 61  HAGRIKIRKFKESAWKCIYFLSAELLALSVTYKESWFTSTKNFWVGPGDQVWPDQRIK 118
           HAGRIKIRKFKESAWKCIYFLSAELLALSVTYKESWFTSTKNFWVGPGDQVWPDQRIK
Sbjct: 61  HAGRIKIRKFKESAWKCIYFLSAELLALSVTYKESWFTSTKNFWVGPGDQVWPDQRIK 118



>Os02g0581300 TRAM, LAG1 and CLN8 homology domain containing protein
          Length = 309

 Score =  170 bits (431), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 82/113 (72%), Positives = 92/113 (81%), Gaps = 1/113 (0%)

Query: 6   VDWEAESYPAYSDFAAIPLFAVFLFAVRYLLDRFVFEWLARRLIFEKDEKLDLATHAGRI 65
           VDWE E+YPAY DF A+PLFAVF   VRYLLD FVFEW+ R+LIF K EK+D      R 
Sbjct: 14  VDWEREAYPAYRDFFALPLFAVFFLVVRYLLDCFVFEWIGRKLIFGK-EKVDYEKEETRK 72

Query: 66  KIRKFKESAWKCIYFLSAELLALSVTYKESWFTSTKNFWVGPGDQVWPDQRIK 118
           KIRKFKESAWKC+YFLS E+L+LSVTY E WFT+TK FWVGPGDQVWPDQ+IK
Sbjct: 73  KIRKFKESAWKCVYFLSGEILSLSVTYNEPWFTNTKYFWVGPGDQVWPDQKIK 125



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.326    0.140    0.463 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,148,750
Number of extensions: 134319
Number of successful extensions: 312
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 312
Number of HSP's successfully gapped: 2
Length of query: 118
Length of database: 17,035,801
Length adjustment: 85
Effective length of query: 33
Effective length of database: 12,597,611
Effective search space: 415721163
Effective search space used: 415721163
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 149 (62.0 bits)