BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0644200 Os02g0644200|AK111133
         (128 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0644200  Protein of unknown function DUF125, transmembr...   135   5e-33
Os04g0538400  Similar to Nodulin 21 (N-21)                         64   2e-11

>Os02g0644200 Protein of unknown function DUF125, transmembrane family protein
          Length = 128

 Score =  135 bits (341), Expect = 5e-33,   Method: Compositional matrix adjust.
 Identities = 78/128 (60%), Positives = 78/128 (60%)

Query: 1   AIGEFVSVYAQCDIQAAQIXXXXXXXXXXXXXXXXXLPSPTMXXXXXXXXXXXXXXXXXX 60
           AIGEFVSVYAQCDIQAAQI                 LPSPTM                  
Sbjct: 1   AIGEFVSVYAQCDIQAAQIERARGGKDADGGEEEEELPSPTMAAVASALSFAAGAALPLL 60

Query: 61  XXXXXRPWXXXXXXXXXXSSLGLAGFGVASAYLGGAGVARSGVRMLVGGWLAMAVTYGVL 120
                RPW          SSLGLAGFGVASAYLGGAGVARSGVRMLVGGWLAMAVTYGVL
Sbjct: 61  AGGFVRPWAARVAAVCAASSLGLAGFGVASAYLGGAGVARSGVRMLVGGWLAMAVTYGVL 120

Query: 121 KLFGMHGV 128
           KLFGMHGV
Sbjct: 121 KLFGMHGV 128



>Os04g0538400 Similar to Nodulin 21 (N-21)
          Length = 247

 Score = 64.3 bits (155), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 55/130 (42%), Positives = 64/130 (49%), Gaps = 2/130 (1%)

Query: 1   AIGEFVSVYAQCDIQAAQIXXXXXXXXXXXXXXXXXLPSPTMXXXXXXXXXXXXXXXXXX 60
           AIGEFVSVYAQ DI+  QI                 LPSPT                   
Sbjct: 112 AIGEFVSVYAQYDIEVTQIERDGDIDGADAAAAREKLPSPTQAAFASALAFAIGGLLPLL 171

Query: 61  XXXXXRPWXXXXXXXXXXSSLGLAGFGVASAYLGGAGVARSGVRMLVGGWLAMAVTYGVL 120
                +PW          SS+GLAGFG A  YLGGA + RSG R+L+GGWLAM +TY VL
Sbjct: 172 TSGFIKPWGPRVGVVCAASSVGLAGFGAAGGYLGGANMVRSGTRVLLGGWLAMLITYAVL 231

Query: 121 KLFG--MHGV 128
           +LF    HG+
Sbjct: 232 RLFATIFHGM 241



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.139    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,456,187
Number of extensions: 59164
Number of successful extensions: 323
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 321
Number of HSP's successfully gapped: 3
Length of query: 128
Length of database: 17,035,801
Length adjustment: 89
Effective length of query: 39
Effective length of database: 12,388,755
Effective search space: 483161445
Effective search space used: 483161445
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 150 (62.4 bits)