BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0635000 Os02g0635000|AK069855
         (357 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0635000  Similar to Nitrilase 1                              731   0.0  
Os02g0635200  Similar to Nitrilase 2 (EC 3.5.5.1)                 580   e-166

>Os02g0635000 Similar to Nitrilase 1
          Length = 357

 Score =  731 bits (1886), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 357/357 (100%), Positives = 357/357 (100%)

Query: 1   MALAPASSGSGPVVTEVEMNAGADQGATGTVRATVVQACTVFYDTPATLDKAEKLVAEAA 60
           MALAPASSGSGPVVTEVEMNAGADQGATGTVRATVVQACTVFYDTPATLDKAEKLVAEAA
Sbjct: 1   MALAPASSGSGPVVTEVEMNAGADQGATGTVRATVVQACTVFYDTPATLDKAEKLVAEAA 60

Query: 61  GYGSQIVLFPEVFVGGYPHGSTFGLTIGNRSAKGKEDFHKYHAAAIDVPGPEVTRLAALA 120
           GYGSQIVLFPEVFVGGYPHGSTFGLTIGNRSAKGKEDFHKYHAAAIDVPGPEVTRLAALA
Sbjct: 61  GYGSQIVLFPEVFVGGYPHGSTFGLTIGNRSAKGKEDFHKYHAAAIDVPGPEVTRLAALA 120

Query: 121 GKYKIFLVMGVVERVGYTLYNTVLFFDPLGKYLGKHRKVMPTGLERVFWGFGDGSTIPVY 180
           GKYKIFLVMGVVERVGYTLYNTVLFFDPLGKYLGKHRKVMPTGLERVFWGFGDGSTIPVY
Sbjct: 121 GKYKIFLVMGVVERVGYTLYNTVLFFDPLGKYLGKHRKVMPTGLERVFWGFGDGSTIPVY 180

Query: 181 DTPIGKIGAVICWENRMPLLRTAMYAKGVQIYCAPTADFSPSWQASMTHIAVEGGCFVLS 240
           DTPIGKIGAVICWENRMPLLRTAMYAKGVQIYCAPTADFSPSWQASMTHIAVEGGCFVLS
Sbjct: 181 DTPIGKIGAVICWENRMPLLRTAMYAKGVQIYCAPTADFSPSWQASMTHIAVEGGCFVLS 240

Query: 241 ANQFCRRKNYPPAPEYTFGGLDEEPSPESVIWPGGSSIVSPSGTVLAGPNYEGEGLLTAD 300
           ANQFCRRKNYPPAPEYTFGGLDEEPSPESVIWPGGSSIVSPSGTVLAGPNYEGEGLLTAD
Sbjct: 241 ANQFCRRKNYPPAPEYTFGGLDEEPSPESVIWPGGSSIVSPSGTVLAGPNYEGEGLLTAD 300

Query: 301 LDLGEIARAKFDFDVVGHYARPEVLSLTVKTESKLPVSFTSAAEKNSAAKSDGISRT 357
           LDLGEIARAKFDFDVVGHYARPEVLSLTVKTESKLPVSFTSAAEKNSAAKSDGISRT
Sbjct: 301 LDLGEIARAKFDFDVVGHYARPEVLSLTVKTESKLPVSFTSAAEKNSAAKSDGISRT 357



>Os02g0635200 Similar to Nitrilase 2 (EC 3.5.5.1)
          Length = 362

 Score =  580 bits (1495), Expect = e-166,   Method: Compositional matrix adjust.
 Identities = 279/361 (77%), Positives = 314/361 (86%), Gaps = 6/361 (1%)

Query: 1   MALAPASSGSGP-VVTEVEMNAGADQGATGTVRATVVQACTVFYDTPATLDKAEKLVAEA 59
           MA+ P+ SG GP V+ EVEMN GAD GA  TVRATVVQA TVFYDTPATLDKAE+L+ EA
Sbjct: 1   MAMVPSGSGGGPPVIAEVEMNGGADSGAA-TVRATVVQASTVFYDTPATLDKAERLIEEA 59

Query: 60  AGYGSQIVLFPEVFVGGYPHGSTFG----LTIGNRSAKGKEDFHKYHAAAIDVPGPEVTR 115
           AGYGSQ+V+FPE FVGGYP GSTFG    ++IGN   KGKE+F KYHAAAI+VPGPEVTR
Sbjct: 60  AGYGSQLVVFPEAFVGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTR 119

Query: 116 LAALAGKYKIFLVMGVVERVGYTLYNTVLFFDPLGKYLGKHRKVMPTGLERVFWGFGDGS 175
           LAA+AGKYK+FLVMGV+ER GYTLY +VLFFDPLG+YLGKHRK+MPT LER+ WGFGDGS
Sbjct: 120 LAAMAGKYKVFLVMGVIEREGYTLYCSVLFFDPLGRYLGKHRKLMPTALERIIWGFGDGS 179

Query: 176 TIPVYDTPIGKIGAVICWENRMPLLRTAMYAKGVQIYCAPTADFSPSWQASMTHIAVEGG 235
           TIPVYDTP+GKIGA+ICWEN+MPLLRTA+Y KG++IYCAPTAD    WQASMTHIA+EGG
Sbjct: 180 TIPVYDTPLGKIGALICWENKMPLLRTALYGKGIEIYCAPTADSRQVWQASMTHIALEGG 239

Query: 236 CFVLSANQFCRRKNYPPAPEYTFGGLDEEPSPESVIWPGGSSIVSPSGTVLAGPNYEGEG 295
           CFVLSANQFCRRK+YPP PEY F GL EEPSP++V+ PGGS I+SPSG VLAGPNYEGE 
Sbjct: 240 CFVLSANQFCRRKDYPPPPEYVFSGLGEEPSPDTVVCPGGSVIISPSGEVLAGPNYEGEA 299

Query: 296 LLTADLDLGEIARAKFDFDVVGHYARPEVLSLTVKTESKLPVSFTSAAEKNSAAKSDGIS 355
           L+TADLDLGEI RAKFDFDVVGHYARPEVLSL V  +  LPVSFTSAAEK +AAKSD  +
Sbjct: 300 LITADLDLGEIVRAKFDFDVVGHYARPEVLSLVVNDQPHLPVSFTSAAEKTTAAKSDSTA 359

Query: 356 R 356
           +
Sbjct: 360 K 360



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.135    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 13,504,883
Number of extensions: 640862
Number of successful extensions: 1223
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1222
Number of HSP's successfully gapped: 3
Length of query: 357
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 255
Effective length of database: 11,709,973
Effective search space: 2986043115
Effective search space used: 2986043115
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)