BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0605400 Os02g0605400|Os02g0605400
         (461 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0605400  C2 calcium/lipid-binding region, CaLB domain c...   783   0.0  
Os09g0516900  C2 calcium/lipid-binding region, CaLB domain c...   197   1e-50

>Os02g0605400 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 461

 Score =  783 bits (2022), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 387/461 (83%), Positives = 387/461 (83%)

Query: 1   MEAAGLSSWMRGFLLPSXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXXXXXXX 60
           MEAAGLSSWMRGFLLPS                           Q               
Sbjct: 1   MEAAGLSSWMRGFLLPSLWEAEVAVSAAALLVAALLLLFLDQAVQSSTKSPASSSSPPPS 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXELGGTSKVALPDGSPHSRGRTSYVIKLE 120
                                           ELGGTSKVALPDGSPHSRGRTSYVIKLE
Sbjct: 61  PTTTAAASCRRDGGCGGCGCRRRRAKGKPAAAELGGTSKVALPDGSPHSRGRTSYVIKLE 120

Query: 121 LLCAKYLIGANLNGSSDPYAVISCGEQRRFSSMVPSSRNPLWGEEFNFLVRELPVEFCTA 180
           LLCAKYLIGANLNGSSDPYAVISCGEQRRFSSMVPSSRNPLWGEEFNFLVRELPVEFCTA
Sbjct: 121 LLCAKYLIGANLNGSSDPYAVISCGEQRRFSSMVPSSRNPLWGEEFNFLVRELPVEFCTA 180

Query: 181 PVNDSKVTITMYDWDTVCKCKVIGSVTVAVLGEDEAGATWFDLDSKSGQICLRFSSAKVF 240
           PVNDSKVTITMYDWDTVCKCKVIGSVTVAVLGEDEAGATWFDLDSKSGQICLRFSSAKVF
Sbjct: 181 PVNDSKVTITMYDWDTVCKCKVIGSVTVAVLGEDEAGATWFDLDSKSGQICLRFSSAKVF 240

Query: 241 LTSERLFTKVILVLWRGLSCTMGIKRSQHSLINPAITIFLRTGSGGHGTPPSCSQNGRIR 300
           LTSERLFTKVILVLWRGLSCTMGIKRSQHSLINPAITIFLRTGSGGHGTPPSCSQNGRIR
Sbjct: 241 LTSERLFTKVILVLWRGLSCTMGIKRSQHSLINPAITIFLRTGSGGHGTPPSCSQNGRIR 300

Query: 301 YKFTSFWNRNRTFRALENALQSYRATLEAEKQVRMHSLQQRRSSDVICSKIDDLKTAERS 360
           YKFTSFWNRNRTFRALENALQSYRATLEAEKQVRMHSLQQRRSSDVICSKIDDLKTAERS
Sbjct: 301 YKFTSFWNRNRTFRALENALQSYRATLEAEKQVRMHSLQQRRSSDVICSKIDDLKTAERS 360

Query: 361 IEQAKAFQPFINEHVLVDATSLSKWYPSDEYGGKIREVMFRSLCHSPLCPPDTAVTEWQR 420
           IEQAKAFQPFINEHVLVDATSLSKWYPSDEYGGKIREVMFRSLCHSPLCPPDTAVTEWQR
Sbjct: 361 IEQAKAFQPFINEHVLVDATSLSKWYPSDEYGGKIREVMFRSLCHSPLCPPDTAVTEWQR 420

Query: 421 ASFSKNKTNLIYETKHQAHDVPFGSYFEVASKNNLQFNMSS 461
           ASFSKNKTNLIYETKHQAHDVPFGSYFEVASKNNLQFNMSS
Sbjct: 421 ASFSKNKTNLIYETKHQAHDVPFGSYFEVASKNNLQFNMSS 461



>Os09g0516900 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 612

 Score =  197 bits (501), Expect = 1e-50,   Method: Compositional matrix adjust.
 Identities = 102/239 (42%), Positives = 140/239 (58%), Gaps = 37/239 (15%)

Query: 246 LFTKVILVLWRGLSCTMGIKRSQHSLINPAITIFLRTGSGGHGTPPSCSQNGRIRYKFTS 305
           +F+K I V+   L     I+RSQH++INPAITIFLR G+GGHG PP  S +GR+RYKF S
Sbjct: 283 VFSKQIKVML-PLRDIDEIRRSQHAVINPAITIFLRMGAGGHGVPPLGSPDGRVRYKFAS 341

Query: 306 FWNRNRTFRALENALQSYRATLEAEKQVRMHSLQQRRSSDVICSKIDDLKTAERSIEQAK 365
           FWNRN TFRAL+ A++++   +EAEKQ R  S  +  SS    S + ++   E   +   
Sbjct: 342 FWNRNHTFRALQRAVKNFHTMIEAEKQERAQSALRAHSSSRK-SSMKEINVPEDCADLTG 400

Query: 366 AFQPFINEHVLVDA-----------------------------------TSLSKWYPSDE 390
             QPF+ E VLV                                      +L +W+ +DE
Sbjct: 401 QLQPFVKEEVLVTVFNGSFPCTAEEFFNTLLSDDSSYITEFRTARKDKDINLGQWHHADE 460

Query: 391 YGGKIREVMFRSLCHSPLCPPDTAVTEWQRASFSKNKTNLIYETKHQAHDVPFGSYFEV 449
           Y G++RE+  +S+CHSP+CPP +A+TEWQ    S +KT+L++ET  Q HDVPFGS+FEV
Sbjct: 461 YDGQVRELNCKSICHSPMCPPYSAMTEWQHMVLSADKTDLVFETVQQVHDVPFGSFFEV 519




 Score =  164 bits (416), Expect = 9e-41,   Method: Compositional matrix adjust.
 Identities = 78/137 (56%), Positives = 97/137 (70%), Gaps = 10/137 (7%)

Query: 109 SRGRTSYVIKLELLCAKYLIGANLNGSSDPYAVISCGEQRRFSSMVPSSRNPLWGEEFNF 168
           S G ++YV+KLELL AK LI ANLNG+SDPYA+I+CGE++RFSSMVP SRNP+WGEEFNF
Sbjct: 83  SSGPSAYVVKLELLAAKNLIAANLNGTSDPYALITCGEEKRFSSMVPGSRNPMWGEEFNF 142

Query: 169 LVRELPVEFCTAPVNDSKVTITMYDWDTVCKCKVIGSVTVAVLGEDEAGATWFDLDSKSG 228
            V  LPV          K+ +T+YDWD V K  V+GSV V V  E ++G  W+ LDS SG
Sbjct: 143 FVDSLPV----------KINVTIYDWDIVWKSTVLGSVIVPVESEGQSGPVWYTLDSTSG 192

Query: 229 QICLRFSSAKVFLTSER 245
           Q+CL   + KV  +S R
Sbjct: 193 QVCLHIKAIKVHESSSR 209



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.132    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 13,569,110
Number of extensions: 529673
Number of successful extensions: 1172
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1171
Number of HSP's successfully gapped: 4
Length of query: 461
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 357
Effective length of database: 11,605,545
Effective search space: 4143179565
Effective search space used: 4143179565
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 158 (65.5 bits)