BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0300700 Os02g0300700|AK069347
         (142 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0300700  Similar to Eukaryotic translation initiation f...   229   5e-61
Os06g0342200  Similar to Eukaryotic translation initiation f...   208   1e-54

>Os02g0300700 Similar to Eukaryotic translation initiation factor 1A (EIF-1A)
           (EIF-4C)
          Length = 142

 Score =  229 bits (584), Expect = 5e-61,   Method: Compositional matrix adjust.
 Identities = 117/142 (82%), Positives = 117/142 (82%)

Query: 1   MPXXXXXXXXXXXXXXXEADDEKRELVFKEDGQEYAQVARMLGNGRCEAQCIDGTKRLCH 60
           MP               EADDEKRELVFKEDGQEYAQVARMLGNGRCEAQCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVARMLGNGRCEAQCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMNDEARLLKAYGEIPDHVRLNEGVV 120
           IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMNDEARLLKAYGEIPDHVRLNEGVV
Sbjct: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMNDEARLLKAYGEIPDHVRLNEGVV 120

Query: 121 XXXXXXXXXXYIQFEDEDIDKI 142
                     YIQFEDEDIDKI
Sbjct: 121 DEDDAAAHDDYIQFEDEDIDKI 142



>Os06g0342200 Similar to Eukaryotic translation initiation factor 1A (EIF-1A)
           (EIF-4C)
          Length = 144

 Score =  208 bits (529), Expect = 1e-54,   Method: Compositional matrix adjust.
 Identities = 108/144 (75%), Positives = 113/144 (78%), Gaps = 2/144 (1%)

Query: 1   MPXXXXXXXXXXXXXXXEADDEKRELVFKEDGQEYAQVARMLGNGRCEAQCIDGTKRLCH 60
           MP               EADD+KRELVFKEDGQEYAQV RMLGNGRCEA C+DGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDDKRELVFKEDGQEYAQVTRMLGNGRCEAICVDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMNDEARLLKAYGEIPDHVRLNEG-- 118
           IRGKMHKKVWIAAGDI+LVGLRDYQDDKADVILKYMNDEARLLKAYGE+PD +RLNEG  
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMNDEARLLKAYGELPDTLRLNEGVD 120

Query: 119 VVXXXXXXXXXXYIQFEDEDIDKI 142
           V           YIQFEDEDIDKI
Sbjct: 121 VDGPEDGGDHDDYIQFEDEDIDKI 144



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.141    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,237,913
Number of extensions: 163872
Number of successful extensions: 307
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 306
Number of HSP's successfully gapped: 2
Length of query: 142
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 51
Effective length of database: 12,284,327
Effective search space: 626500677
Effective search space used: 626500677
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 151 (62.8 bits)