BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0257300 Os02g0257300|Os02g0257300
         (478 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0257300  Rhodanese-like domain containing protein            461   e-130
Os01g0889800  Rhodanese-like domain containing protein             97   2e-20

>Os02g0257300 Rhodanese-like domain containing protein
          Length = 478

 Score =  461 bits (1186), Expect = e-130,   Method: Compositional matrix adjust.
 Identities = 264/406 (65%), Positives = 264/406 (65%)

Query: 1   MAATTTILSSXXXXXXXXXXXXXXXXXXXXXXXXXXXDIXXXXXXXXXXXXXXXXXXPLS 60
           MAATTTILSS                           DI                  PLS
Sbjct: 1   MAATTTILSSAAPTPLTAPPRARARAPAARRRRLRARDILGAALGLANGGASAALAAPLS 60

Query: 61  YEETLRLSTDSXXXXXXXXXXEFALPDLGLGGVLDFXXXXXXXXXXXXXXXXXXXXXXXX 120
           YEETLRLSTDS          EFALPDLGLGGVLDF                        
Sbjct: 61  YEETLRLSTDSGGGGGGGGGGEFALPDLGLGGVLDFVAQNPLAAAAGVAAVALPLVLAQV 120

Query: 121 XXXXSKPYGVVSAAAAYRALVEEPGAQLVDIRPPGDARQSGAPDLREAKKKAAAVPYDGE 180
               SKPYGVVSAAAAYRALVEEPGAQLVDIRPPGDARQSGAPDLREAKKKAAAVPYDGE
Sbjct: 121 LGGASKPYGVVSAAAAYRALVEEPGAQLVDIRPPGDARQSGAPDLREAKKKAAAVPYDGE 180

Query: 181 DKNGFLKKLSLRFKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWL 240
           DKNGFLKKLSLRFKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWL
Sbjct: 181 DKNGFLKKLSLRFKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWL 240

Query: 241 SSSLPWTAPKKGFSLSDLIGDGTDGLPVXXXXXXXXXXXXXXXXEIETVLQFLGSAAIVQ 300
           SSSLPWTAPKKGFSLSDLIGDGTDGLPV                EIETVLQFLGSAAIVQ
Sbjct: 241 SSSLPWTAPKKGFSLSDLIGDGTDGLPVTLGLAAATGLGILAYTEIETVLQFLGSAAIVQ 300

Query: 301 LVASKLIYAEDRKRTLKQIDDFFNKKVAPKELVDEIKEIGQALLPSTGTKSQXXXXXXXX 360
           LVASKLIYAEDRKRTLKQIDDFFNKKVAPKELVDEIKEIGQALLPSTGTKSQ        
Sbjct: 301 LVASKLIYAEDRKRTLKQIDDFFNKKVAPKELVDEIKEIGQALLPSTGTKSQPAITEAAP 360

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPYTNYPDLK 406
                                              LSPYTNYPDLK
Sbjct: 361 ATAEAAPAAATATAAPPAAPVEETSTEAAPAEPTPLSPYTNYPDLK 406



>Os01g0889800 Rhodanese-like domain containing protein
          Length = 361

 Score = 97.4 bits (241), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 65/148 (43%), Positives = 85/148 (57%), Gaps = 9/148 (6%)

Query: 126 KPYGVVSAAAAYRALVEEPGAQLVDIRPPGDARQSGAPDLREAKKKAAAVPYDGEDKNGF 185
           K Y  VSA  A+R L +EPGAQL+DIR     R   +P+LR  +K A  V +D ED+ GF
Sbjct: 146 KKYKAVSAIDAFRKLRDEPGAQLLDIRRGKSVRFMASPNLRLVEKSAVQVEFDEEDEEGF 205

Query: 186 LKKLSLRFKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWLSSSLP 245
           +K++  RF DP NT + +LD FDGNS  VAEL+  NG+K A+A+K G  G  GW +    
Sbjct: 206 VKEVLARFPDPANTVVCVLDNFDGNSMKVAELLFNNGFKEAYAIKGGLRGPEGWQAIQEK 265

Query: 246 WTAP---------KKGFSLSDLIGDGTD 264
           +  P          K    SD+  DGTD
Sbjct: 266 YLPPSVHVVPRKKSKESEDSDVSADGTD 293



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.134    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,138,830
Number of extensions: 324756
Number of successful extensions: 708
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 705
Number of HSP's successfully gapped: 2
Length of query: 478
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 373
Effective length of database: 11,553,331
Effective search space: 4309392463
Effective search space used: 4309392463
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 158 (65.5 bits)