BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0971600 Os01g0971600|AK070366
         (351 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0971600  Similar to Sn-glycerol-3-phosphate dehydrogena...   721   0.0  
Os07g0229800  NAD-dependent glycerol-3-phosphate dehydrogena...    94   2e-19

>Os01g0971600 Similar to Sn-glycerol-3-phosphate dehydrogenase (Fragment)
          Length = 351

 Score =  721 bits (1862), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 351/351 (100%), Positives = 351/351 (100%)

Query: 1   MENGHAKNLVAVIGSGNWGSVASRLIASNTAKLPSFHDEVRMWVFEEILPTGKKLSESIN 60
           MENGHAKNLVAVIGSGNWGSVASRLIASNTAKLPSFHDEVRMWVFEEILPTGKKLSESIN
Sbjct: 1   MENGHAKNLVAVIGSGNWGSVASRLIASNTAKLPSFHDEVRMWVFEEILPTGKKLSESIN 60

Query: 61  QANENCKYLPGIKLGANVIADPDLENAVKDANMLVFVTPHQFVEGICKKLVGKLRPGTEG 120
           QANENCKYLPGIKLGANVIADPDLENAVKDANMLVFVTPHQFVEGICKKLVGKLRPGTEG
Sbjct: 61  QANENCKYLPGIKLGANVIADPDLENAVKDANMLVFVTPHQFVEGICKKLVGKLRPGTEG 120

Query: 121 ISLIKGMEVKMEGPCMISKLITNILGINCCVLMGANIANEIAVEKFSEATIGYKKDKEVA 180
           ISLIKGMEVKMEGPCMISKLITNILGINCCVLMGANIANEIAVEKFSEATIGYKKDKEVA
Sbjct: 121 ISLIKGMEVKMEGPCMISKLITNILGINCCVLMGANIANEIAVEKFSEATIGYKKDKEVA 180

Query: 181 TRWAKLFTTPYFLVSVVEDIEGVELCGTLKNVVAIAAGLVDGLDMGNNTKAAIMRIGLRE 240
           TRWAKLFTTPYFLVSVVEDIEGVELCGTLKNVVAIAAGLVDGLDMGNNTKAAIMRIGLRE
Sbjct: 181 TRWAKLFTTPYFLVSVVEDIEGVELCGTLKNVVAIAAGLVDGLDMGNNTKAAIMRIGLRE 240

Query: 241 MRAFSKLLSPTVRDNTFFESCGVADLITTCLGGRNRRVAEAFARNGGKRSFDELEAEMLH 300
           MRAFSKLLSPTVRDNTFFESCGVADLITTCLGGRNRRVAEAFARNGGKRSFDELEAEMLH
Sbjct: 241 MRAFSKLLSPTVRDNTFFESCGVADLITTCLGGRNRRVAEAFARNGGKRSFDELEAEMLH 300

Query: 301 GQKLQGVSTAKEVYEVLTYRGWQELFPLLSTVHEICIGQLPPTSIVEYRIH 351
           GQKLQGVSTAKEVYEVLTYRGWQELFPLLSTVHEICIGQLPPTSIVEYRIH
Sbjct: 301 GQKLQGVSTAKEVYEVLTYRGWQELFPLLSTVHEICIGQLPPTSIVEYRIH 351



>Os07g0229800 NAD-dependent glycerol-3-phosphate dehydrogenase family protein
          Length = 254

 Score = 93.6 bits (231), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 81/250 (32%), Positives = 120/250 (48%), Gaps = 21/250 (8%)

Query: 102 FVEGICKKLVGKLRPGTEGISLIKGMEVKMEGPCMISKLITNILG---INCCVLMGANIA 158
           F+EGI   +     P    ISL KG+E+       +S++I   LG       VL G + A
Sbjct: 9   FLEGISTHV----DPKLPFISLSKGLELNTLR--TMSQIIPQALGNPRQPFIVLSGPSFA 62

Query: 159 NEIAVEKFSEATIGYKKDKEVATRWAKLFTTPYFLVSVVEDIEGVELCGTLKNVVAIAAG 218
            E+ + K   A +   KDK++A    +L  +P   +S   D+ GVE+ G LKNV+AIAAG
Sbjct: 63  IEL-MNKLPTAMVVASKDKKLAAAVQQLLASPNLRISTSNDVTGVEIAGALKNVLAIAAG 121

Query: 219 LVDGLDMGNNTKAAIMRIGLREMRAFSKLLSPTVRDNTFFESCGVADLITTCLG--GRNR 276
           +V+G+ +GNN  AA++  G  E+R  +  +    +  T     G  D++ TC     RNR
Sbjct: 122 IVEGMHLGNNCMAALVAQGCSEIRWLATKMG--AKPTTLSGLSGSGDIMLTCFVNLSRNR 179

Query: 277 RVAEAFARNGGKRSFDELEAEMLHGQKLQGVSTAKEVYEVLTYRGWQELFPLLSTVHEIC 336
            V     R G     DE+   M   Q  +GVSTA  V  +   + +    P+L+ V  I 
Sbjct: 180 NVG---LRLGSGEKLDEIMNSM--NQVAEGVSTAGAVIAL--AQKYHVKMPVLTAVARII 232

Query: 337 IGQLPPTSIV 346
             +L P   V
Sbjct: 233 DNELTPKKAV 242



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,310,085
Number of extensions: 442462
Number of successful extensions: 955
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 955
Number of HSP's successfully gapped: 2
Length of query: 351
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 249
Effective length of database: 11,709,973
Effective search space: 2915783277
Effective search space used: 2915783277
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)