BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0878800 Os01g0878800|AK070251
         (322 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0878800  Similar to 4,5-DOPA dioxygenase extradiol           544   e-155
Os01g0878900  Similar to 4,5-DOPA dioxygenase extradiol-like...   444   e-125
AK109035                                                          120   2e-27

>Os01g0878800 Similar to 4,5-DOPA dioxygenase extradiol
          Length = 322

 Score =  544 bits (1401), Expect = e-155,   Method: Compositional matrix adjust.
 Identities = 271/322 (84%), Positives = 271/322 (84%)

Query: 1   MGQRQSKQXXXXXXXXXXXXXXXXXXXGEDEQQGSALXXXXXXXXXXXXXXXXXXXMDTF 60
           MGQRQSKQ                   GEDEQQGSAL                   MDTF
Sbjct: 1   MGQRQSKQPSPAAAVHARATTTPTPTPGEDEQQGSALEAERRGRRRTTTRRAAEGAMDTF 60

Query: 61  FLSHGAPTLSIDDTIAAQGFFKSWLPAAVAGAELPRAILVVSGHWEAAAPTVNVIRGNND 120
           FLSHGAPTLSIDDTIAAQGFFKSWLPAAVAGAELPRAILVVSGHWEAAAPTVNVIRGNND
Sbjct: 61  FLSHGAPTLSIDDTIAAQGFFKSWLPAAVAGAELPRAILVVSGHWEAAAPTVNVIRGNND 120

Query: 121 TIHDFYGFPKAMYKLKYPAPGAPDLAMKTKELLEQAGFGPVKENHSRGLDHGAWVPLMFM 180
           TIHDFYGFPKAMYKLKYPAPGAPDLAMKTKELLEQAGFGPVKENHSRGLDHGAWVPLMFM
Sbjct: 121 TIHDFYGFPKAMYKLKYPAPGAPDLAMKTKELLEQAGFGPVKENHSRGLDHGAWVPLMFM 180

Query: 181 YPEANVPVCQLSLQSGRDGAYHYELGRALAPLRDDGVLVLGSGSATHNLRRMGPEGTPVP 240
           YPEANVPVCQLSLQSGRDGAYHYELGRALAPLRDDGVLVLGSGSATHNLRRMGPEGTPVP
Sbjct: 181 YPEANVPVCQLSLQSGRDGAYHYELGRALAPLRDDGVLVLGSGSATHNLRRMGPEGTPVP 240

Query: 241 QWAAEFDGWLQEALLGGRHDDVKRYEEKAPHGRVAHPSPDHFLPLHVXXXXXXXXXXXXX 300
           QWAAEFDGWLQEALLGGRHDDVKRYEEKAPHGRVAHPSPDHFLPLHV             
Sbjct: 241 QWAAEFDGWLQEALLGGRHDDVKRYEEKAPHGRVAHPSPDHFLPLHVALGAAGEGAKAEL 300

Query: 301 IHRSWSNASLSYASYRFTTAKN 322
           IHRSWSNASLSYASYRFTTAKN
Sbjct: 301 IHRSWSNASLSYASYRFTTAKN 322



>Os01g0878900 Similar to 4,5-DOPA dioxygenase extradiol-like protein
          Length = 280

 Score =  444 bits (1142), Expect = e-125,   Method: Compositional matrix adjust.
 Identities = 215/268 (80%), Positives = 236/268 (88%), Gaps = 5/268 (1%)

Query: 57  MDTFFLSHGAPTLSIDDTIAAQGFFKSWLPAAVAGAELPRAILVVSGHWEAAAPTVNVIR 116
           MDTFFLSHG PTLSIDDT+ AQ FF+SWLPAAVAGA+ PRAIL+VSGHWE A PTVNV+R
Sbjct: 1   MDTFFLSHGTPTLSIDDTMPAQHFFRSWLPAAVAGAQPPRAILIVSGHWETATPTVNVVR 60

Query: 117 GNNDTIHDF--YGFPKAMYKLKYPAPGAPDLAMKTKELLEQAGFGPVKENHSRGLDHGAW 174
           GNNDTIHDF  YGFPK+M++L+YPAPGAPD+A K KELLEQAGFGPVKE+H RGLDHGAW
Sbjct: 61  GNNDTIHDFEGYGFPKSMFQLEYPAPGAPDVAKKAKELLEQAGFGPVKEDHGRGLDHGAW 120

Query: 175 VPLMFMYPEANVPVCQLSLQSGRDGAYHYELGRALAPLRDDGVLVLGSGSATHNLRRMGP 234
           VPLMFMYPEANVPVCQLSLQ+GRDGAYHY+LGRALAPLRDDGVL+LGSG+ATHNL  M P
Sbjct: 121 VPLMFMYPEANVPVCQLSLQTGRDGAYHYDLGRALAPLRDDGVLILGSGNATHNLSCMAP 180

Query: 235 --EGTPVPQWAAEFDGWLQEALL-GGRHDDVKRYEEKAPHGRVAHPSPDHFLPLHVXXXX 291
             EGTPVPQWAAEFDGWLQEALL GGRHDDVK+YEEKAP+G++AHPSPDHFLPLHV    
Sbjct: 181 VAEGTPVPQWAAEFDGWLQEALLAGGRHDDVKQYEEKAPNGKMAHPSPDHFLPLHVALGA 240

Query: 292 XXXXXXXXXIHRSWSNASLSYASYRFTT 319
                    IH SW NA+LS+ASYRFTT
Sbjct: 241 AGEDAKAELIHHSWYNATLSHASYRFTT 268



>AK109035 
          Length = 324

 Score =  120 bits (300), Expect = 2e-27,   Method: Compositional matrix adjust.
 Identities = 72/205 (35%), Positives = 115/205 (56%), Gaps = 11/205 (5%)

Query: 92  AELPRAILVVSGHWEAAAPTVNVIRGNNDTIHDFYGFPKAMYKLKYPAPGAPDLAMKTKE 151
           +E P+AIL+V+ HW     TV+     +D ++D+YGFP   Y++K+ APG+P++A +  +
Sbjct: 94  SEAPKAILLVTAHWSTNKVTVSS-GSKHDLLYDYYGFPPESYEIKHDAPGSPEVAEEVTK 152

Query: 152 LLEQAGFGPVKENHSRGLDHGAWVPLMFMYPEANVPVCQLSLQSGRDGAYHYELGRALAP 211
            L+ AG    K+   RG DHG +VP+  + P A  P+ QLS+ +    +  Y +G+AL+ 
Sbjct: 153 ALKAAGIECAKDGE-RGWDHGVFVPMKLIDPSARTPIVQLSVLASESPSQSYAIGKALSS 211

Query: 212 LRDDGVLVLGSGSAT-HNLRRMGPEGTPVPQWAAEFDGW---LQEALLGGRHD-DVKRYE 266
           LR   + ++GSG AT HNLR M    +  P +  +   W   + +A + G  D   +++E
Sbjct: 212 LRAQNIAIVGSGFATFHNLRLMFSGASSTPDFKKQNVEWPAAVTDAAMTGDADARARKFE 271

Query: 267 E--KAPHGRVAHP--SPDHFLPLHV 287
           +  K P     HP    +HFLPL V
Sbjct: 272 DWRKWPGAYTMHPRGGAEHFLPLIV 296



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.135    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,959,504
Number of extensions: 505421
Number of successful extensions: 995
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 989
Number of HSP's successfully gapped: 3
Length of query: 322
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 221
Effective length of database: 11,762,187
Effective search space: 2599443327
Effective search space used: 2599443327
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)