BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0864700 Os01g0864700|J033124A14
         (449 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0864700  Conserved hypothetical protein                      821   0.0  
Os05g0440800  Protein kinase-like domain containing protein       232   6e-61

>Os01g0864700 Conserved hypothetical protein
          Length = 449

 Score =  821 bits (2121), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 411/449 (91%), Positives = 411/449 (91%)

Query: 1   MEDVDAAVGKVKNRTXXXXXXXXXXXXXXXLGAESPLHADTKRAHNKVIKERAKSPCRPV 60
           MEDVDAAVGKVKNRT               LGAESPLHADTKRAHNKVIKERAKSPCRPV
Sbjct: 1   MEDVDAAVGKVKNRTPPSYSRRLSIPPRRPLGAESPLHADTKRAHNKVIKERAKSPCRPV 60

Query: 61  HGPDNDIIEPPGFPMAPPSPLGGVQMKVGNARAKSAPPRAVSIKEXXXXXXXXTIAYAEN 120
           HGPDNDIIEPPGFPMAPPSPLGGVQMKVGNARAKSAPPRAVSIKE        TIAYAEN
Sbjct: 61  HGPDNDIIEPPGFPMAPPSPLGGVQMKVGNARAKSAPPRAVSIKEDSSDCSSSTIAYAEN 120

Query: 121 TELSEPSKQDSSAQLVSSCKCSIPDAAIQKHDLTAMPSSELNTTNFQKSMASNDDVCENL 180
           TELSEPSKQDSSAQLVSSCKCSIPDAAIQKHDLTAMPSSELNTTNFQKSMASNDDVCENL
Sbjct: 121 TELSEPSKQDSSAQLVSSCKCSIPDAAIQKHDLTAMPSSELNTTNFQKSMASNDDVCENL 180

Query: 181 ALEPSSDISEQVSIFKDNVPCSKISQSTANAIVQNDEDKFTVQELLSSVADIAPFVSTKN 240
           ALEPSSDISEQVSIFKDNVPCSKISQSTANAIVQNDEDKFTVQELLSSVADIAPFVSTKN
Sbjct: 181 ALEPSSDISEQVSIFKDNVPCSKISQSTANAIVQNDEDKFTVQELLSSVADIAPFVSTKN 240

Query: 241 FALEKGSPPIQSLERTSSPHLNPPIEDVIHVIRHSSFRVCGEQAVAENAEMGVQSSDVGK 300
           FALEKGSPPIQSLERTSSPHLNPPIEDVIHVIRHSSFRVCGEQAVAENAEMGVQSSDVGK
Sbjct: 241 FALEKGSPPIQSLERTSSPHLNPPIEDVIHVIRHSSFRVCGEQAVAENAEMGVQSSDVGK 300

Query: 301 LLNVVREEVDSRSIPSNNLVPHRLPDCAAPKPNISETNTISSKTACSDVVKFLTVPEVNS 360
           LLNVVREEVDSRSIPSNNLVPHRLPDCAAPKPNISETNTISSKTACSDVVKFLTVPEVNS
Sbjct: 301 LLNVVREEVDSRSIPSNNLVPHRLPDCAAPKPNISETNTISSKTACSDVVKFLTVPEVNS 360

Query: 361 TTTAINNGFKEEASPTKEILDVKSFRQRXXXXXXXXXXXXXXXQHNRLEELAVVLKPFGK 420
           TTTAINNGFKEEASPTKEILDVKSFRQR               QHNRLEELAVVLKPFGK
Sbjct: 361 TTTAINNGFKEEASPTKEILDVKSFRQRAEALEGLLELSADLLQHNRLEELAVVLKPFGK 420

Query: 421 DKVSPRETAIWLAKSFKGMMNDEASRSSM 449
           DKVSPRETAIWLAKSFKGMMNDEASRSSM
Sbjct: 421 DKVSPRETAIWLAKSFKGMMNDEASRSSM 449



>Os05g0440800 Protein kinase-like domain containing protein
          Length = 943

 Score =  232 bits (591), Expect = 6e-61,   Method: Compositional matrix adjust.
 Identities = 167/417 (40%), Positives = 225/417 (53%), Gaps = 18/417 (4%)

Query: 41  TKRAHNKVIKERAKSPCRPVHGPDNDIIE-PPGFPMAPPSPLGGVQMKVGNARAKSAPPR 99
           +KRAH K   E  K+P RP H P+N +   PP  P++  + L    +K+GN++A  AP  
Sbjct: 537 SKRAHIKA--ESEKTPPRPAHSPNNSLHNLPPLIPIS--TNLSEENIKLGNSQAMPAPLE 592

Query: 100 AVSI--KEXXXXXXXXTIAYAENTELSEPSKQDSSAQLVSSCKCSIPDAAIQKHDLTAMP 157
            V+   KE         +   E  E SE  + +S A L       + DA   ++ L A+P
Sbjct: 593 FVTAASKEDISFYSNSVVDCVEKAEPSEVFESNSPAYLTPPWTGPVLDAK-GENGLIAIP 651

Query: 158 SSELNTTNFQKSMASNDDVCENLALEPSSDISEQVSIFKDNVPCSKISQSTANAIVQNDE 217
            SE++T   QKSMASNDD   +  L+      EQ  + KD+   SK   S    +  + E
Sbjct: 652 CSEIHTGTLQKSMASNDDSSLSSPLDTFYLSFEQEFVCKDDSQSSKHGHSAVTLL--SGE 709

Query: 218 DKFTVQELLSSVADIAPFVSTKNFALEKGSPPIQSLERTSSPHLNPPIEDVIHVIRHSSF 277
           DKFTVQELL+S   I+PFVS+ +  L +     QS ++ S  H  PP++     IR +SF
Sbjct: 710 DKFTVQELLASTPVISPFVSSTSNTLPEDKSSYQSFKKQSDSHSGPPVDVPAQTIRLNSF 769

Query: 278 RVCGEQAVAENAEMGVQSSDVGKLLNVVREEVDSRSIPSNNLVPHRLPDCAAP---KPNI 334
            V  E   +E  +   + +   KLLNVVRE+ D RS   ++      P    P   K N+
Sbjct: 770 LVSDEWPTSETVQGEARDTAASKLLNVVREDFDVRS---SSCSTSTQPSGQTPVRSKLNV 826

Query: 335 SETNTIS--SKTACSDVVKFLTVPEVNSTTTAINNGFKEEASPTKEILDVKSFRQRXXXX 392
            ETN  S  S  + S+ V+  T  +V   T+  +NG KEEASP KE LDV SFRQR    
Sbjct: 827 PETNLASNISIPSISEAVRLSTAMDVKPYTSEASNGVKEEASPAKEALDVTSFRQRAEAL 886

Query: 393 XXXXXXXXXXXQHNRLEELAVVLKPFGKDKVSPRETAIWLAKSFKGMMNDEASRSSM 449
                      ++NRLEELA+VL+PFGK+KVSPRETAIWLA+SFKGMMN+E  R SM
Sbjct: 887 EGLLELSADLLENNRLEELAIVLQPFGKNKVSPRETAIWLARSFKGMMNEEGGRLSM 943



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.310    0.125    0.350 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 13,807,146
Number of extensions: 534913
Number of successful extensions: 1245
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1242
Number of HSP's successfully gapped: 2
Length of query: 449
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 345
Effective length of database: 11,605,545
Effective search space: 4003913025
Effective search space used: 4003913025
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 158 (65.5 bits)