BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0856900 Os01g0856900|AK107570
         (379 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0856900  Glycoside hydrolase, starch-binding domain con...   619   e-177
Os05g0446900  Glycoside hydrolase, starch-binding domain con...   165   5e-41

>Os01g0856900 Glycoside hydrolase, starch-binding domain containing protein
          Length = 379

 Score =  619 bits (1596), Expect = e-177,   Method: Compositional matrix adjust.
 Identities = 313/379 (82%), Positives = 313/379 (82%)

Query: 1   METAAAVVCXXXXXXXXXXXXXSDSSTTXXXXXXXXXXXXXXXXXXXLLVASLGEPLITA 60
           METAAAVVC             SDSSTT                   LLVASLGEPLITA
Sbjct: 1   METAAAVVCRGGGLRAPARRGGSDSSTTRAGGVAASPAPATTARRRPLLVASLGEPLITA 60

Query: 61  QPLSSSLGDGAAVHETLARSDSVIPSLKPSHCVDHSVQVDADEETGSTKTLPPPDDVPTK 120
           QPLSSSLGDGAAVHETLARSDSVIPSLKPSHCVDHSVQVDADEETGSTKTLPPPDDVPTK
Sbjct: 61  QPLSSSLGDGAAVHETLARSDSVIPSLKPSHCVDHSVQVDADEETGSTKTLPPPDDVPTK 120

Query: 121 TVHVKFVLQKRCAFGQRFLVVGDVAALGLWNPAKAAALDWSEDHVWTVKKELPAERSIEF 180
           TVHVKFVLQKRCAFGQRFLVVGDVAALGLWNPAKAAALDWSEDHVWTVKKELPAERSIEF
Sbjct: 121 TVHVKFVLQKRCAFGQRFLVVGDVAALGLWNPAKAAALDWSEDHVWTVKKELPAERSIEF 180

Query: 181 KFLLQDRSGHVEWQHGRNRILHVADTSNTLIVCEDWDEAKNQQVSEEIGDADGIFSGSDG 240
           KFLLQDRSGHVEWQHGRNRILHVADTSNTLIVCEDWDEAKNQQVSEEIGDADGIFSGSDG
Sbjct: 181 KFLLQDRSGHVEWQHGRNRILHVADTSNTLIVCEDWDEAKNQQVSEEIGDADGIFSGSDG 240

Query: 241 VFXXXXXXXXXXXXTNKGVTVGVDDAKSALVTYIYREMMGANDAIQPQLALDKHHKIPDE 300
           VF            TNKGVTVGVDDAKSALVTYIYREMMGANDAIQPQLALDKHHKIPDE
Sbjct: 241 VFQEDELQLGEEQETNKGVTVGVDDAKSALVTYIYREMMGANDAIQPQLALDKHHKIPDE 300

Query: 301 LSGEANMXXXXXXXXXXXXXXXXXXXXXXEDAILHKEGDPVENNRLGLASIFFNDMAWTR 360
           LSGEANM                      EDAILHKEGDPVENNRLGLASIFFNDMAWTR
Sbjct: 301 LSGEANMAAQDGNHTATAAAASGFAGSNGEDAILHKEGDPVENNRLGLASIFFNDMAWTR 360

Query: 361 KALQQLLRSLGFQIGTRKT 379
           KALQQLLRSLGFQIGTRKT
Sbjct: 361 KALQQLLRSLGFQIGTRKT 379



>Os05g0446900 Glycoside hydrolase, starch-binding domain containing protein
          Length = 373

 Score =  165 bits (418), Expect = 5e-41,   Method: Compositional matrix adjust.
 Identities = 125/352 (35%), Positives = 171/352 (48%), Gaps = 51/352 (14%)

Query: 49  LVASLGEPLITAQPLSSSLGDGAAVHETLARSDSVIPSLKPSHCVDHSVQVDADEETGST 108
           LVA+L EPL   QPLS +     A+  T A               D   +V  D  +   
Sbjct: 48  LVAALPEPL---QPLSPAQDGAVALASTEA---------------DGGEEVHGDVASAEI 89

Query: 109 KTLPPPDDVPTKTVHVKFVLQKRCAFGQRFLVVGDVAALGLWNPAKAAALDWSEDHVWTV 168
            +   P  V  KTV V+FVL++ C FGQ F +VGD  ALGLW+P+KA  LDWSE H WTV
Sbjct: 90  SS---PSGVLGKTVRVRFVLKRECTFGQSFHLVGDDPALGLWDPSKAVPLDWSEGHDWTV 146

Query: 169 KKELPAERSIEFKFLLQDRSGHVEWQHGRNRILHVADTSNTLIVCEDWDEAKNQQVSEE- 227
           +K+LPA + IE+KF+LQD SG + WQ+GRNR +   +T+N L+V EDW  A +Q V EE 
Sbjct: 147 EKDLPANKLIEYKFVLQDLSGKLHWQNGRNRSVQTGETANILVVYEDWGNANSQTVEEEG 206

Query: 228 ---IGDADGIFS--------------GSDGVFXXXXXXXXXXXXTNKGVTVGVDDAKSAL 270
              IG  +G  S                D +                  +V  DD KS +
Sbjct: 207 KVSIGMEEGKLSVGMEEAVVPDDSESRDDIIVADELQVDDNLAVMQNESSVREDDKKSTV 266

Query: 271 --VTYIYREMMGANDAIQPQLALDKHHKIPDELSGEANMXXXXXXXXXXXXXXXXXXXXX 328
             VT +  E+M  ++A QP+L +D+  +I + L   A+                      
Sbjct: 267 GTVTSVQAELMKLHEANQPELIVDE-PQIQEALPETAD--TEPENGGVATCADDRYAEST 323

Query: 329 XEDAI----LHKEGDPVENNRLGLASIFFNDMAWTRKALQQLLRSLGFQIGT 376
            +D +       +G PVEN   G    F +++ W  KALQQLL SLGF++ T
Sbjct: 324 DDDGVPVGGTDDDGVPVENRWTG---AFEHELLWGWKALQQLLMSLGFKMDT 372



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.131    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,069,945
Number of extensions: 436483
Number of successful extensions: 1199
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1197
Number of HSP's successfully gapped: 2
Length of query: 379
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 276
Effective length of database: 11,657,759
Effective search space: 3217541484
Effective search space used: 3217541484
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 157 (65.1 bits)