BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0856800 Os01g0856800|AK063895
         (142 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0856800  Pleckstrin homology-type domain containing pro...   288   1e-78
Os05g0447000  Similar to Pleckstrin homology domain-containi...   196   6e-51

>Os01g0856800 Pleckstrin homology-type domain containing protein
          Length = 142

 Score =  288 bits (737), Expect = 1e-78,   Method: Compositional matrix adjust.
 Identities = 142/142 (100%), Positives = 142/142 (100%)

Query: 1   MAASLWRAVMGAGASSADTDTTGGVEFWRSPERAGWLTKQGEYIKTWRRRWFVLKQGRLF 60
           MAASLWRAVMGAGASSADTDTTGGVEFWRSPERAGWLTKQGEYIKTWRRRWFVLKQGRLF
Sbjct: 1   MAASLWRAVMGAGASSADTDTTGGVEFWRSPERAGWLTKQGEYIKTWRRRWFVLKQGRLF 60

Query: 61  WFKDSGVTVKGAEDTLNRQFAFELSTPAETMYFIADSEKEKEEWINSIGRSIVQHSRSVT 120
           WFKDSGVTVKGAEDTLNRQFAFELSTPAETMYFIADSEKEKEEWINSIGRSIVQHSRSVT
Sbjct: 61  WFKDSGVTVKGAEDTLNRQFAFELSTPAETMYFIADSEKEKEEWINSIGRSIVQHSRSVT 120

Query: 121 DAEVVDYDSGRPAASVTADGDK 142
           DAEVVDYDSGRPAASVTADGDK
Sbjct: 121 DAEVVDYDSGRPAASVTADGDK 142



>Os05g0447000 Similar to Pleckstrin homology domain-containing protein 1 (AtPH1)
          Length = 172

 Score =  196 bits (497), Expect = 6e-51,   Method: Compositional matrix adjust.
 Identities = 107/155 (69%), Positives = 113/155 (72%), Gaps = 24/155 (15%)

Query: 1   MAASLWRAVMGAGASSADTDTTGG-------VEFWRSPERAGWLTKQGEYIKTWRRRWFV 53
           MA SLWRAVMGA      + ++         VEFW   ER GWL KQGEYIKTWRRRWFV
Sbjct: 1   MAVSLWRAVMGAAGGGGSSSSSAAGGDAGGGVEFWHGGERTGWLNKQGEYIKTWRRRWFV 60

Query: 54  LKQGRLFWFKDSGVT----------------VKGAEDTLNRQFAFELSTPAETMYFIADS 97
           LKQGRLFWFKD+ VT                VKGAED +NRQFAFELSTP +TMYFIADS
Sbjct: 61  LKQGRLFWFKDAAVTRGSVPRGVIPVATCLTVKGAEDVINRQFAFELSTPTDTMYFIADS 120

Query: 98  EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDSGRP 132
           EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDS RP
Sbjct: 121 EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDS-RP 154



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.129    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,354,159
Number of extensions: 221656
Number of successful extensions: 624
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 622
Number of HSP's successfully gapped: 2
Length of query: 142
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 51
Effective length of database: 12,284,327
Effective search space: 626500677
Effective search space used: 626500677
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 151 (62.8 bits)