BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0732300 Os01g0732300|AK108391
         (323 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0732300  Protein of unknown function DUF623, plant doma...   379   e-105
Os01g0625900  Protein of unknown function DUF623, plant doma...    74   1e-13
Os01g0226700  Protein of unknown function DUF623, plant doma...    70   1e-12

>Os01g0732300 Protein of unknown function DUF623, plant domain containing protein
          Length = 323

 Score =  379 bits (974), Expect = e-105,   Method: Compositional matrix adjust.
 Identities = 209/323 (64%), Positives = 209/323 (64%)

Query: 1   MDGGGSGAGRKKLKHRLAAILSVFTRRAGDRKRRGEEGXXXXXXXXXXXXXXSYSRLXXX 60
           MDGGGSGAGRKKLKHRLAAILSVFTRRAGDRKRRGEEG              SYSRL   
Sbjct: 1   MDGGGSGAGRKKLKHRLAAILSVFTRRAGDRKRRGEEGAAAPPPPPPPLAFPSYSRLGGA 60

Query: 61  XXXXXXXXXHEXXXXXXXXXXXXXXNITIDCAGRRSVXXXXXXXXXXXXXXXRKMERRLT 120
                    HE              NITIDCAGRRSV               RKMERRLT
Sbjct: 61  GGKKAAAGKHERRLSLSVPRPPPLVNITIDCAGRRSVDAADPSLLAPLDLDARKMERRLT 120

Query: 121 GTGLPYETGEWEGRKCPPSTPFAAAPPLARWKERAXXXXXXXXXXXXXXXXXXXXXXXXX 180
           GTGLPYETGEWEGRKCPPSTPFAAAPPLARWKERA                         
Sbjct: 121 GTGLPYETGEWEGRKCPPSTPFAAAPPLARWKERASVSSRRLSTHSSRRLMSSSSSDDEY 180

Query: 181 XXXXXXXXXXXXXXXXXXXXYNCPRKNTRARASVSGPCRAPPPSSRRGTSQSCRYSFELP 240
                               YNCPRKNTRARASVSGPCRAPPPSSRRGTSQSCRYSFELP
Sbjct: 181 DEDSRNLFSSRSFSSDSSDFYNCPRKNTRARASVSGPCRAPPPSSRRGTSQSCRYSFELP 240

Query: 241 RGSTASAATDGGFAVVKRSSDPYEDFRKSMEEMIXXXXXXXXXXXXXXLSAERLLETYLV 300
           RGSTASAATDGGFAVVKRSSDPYEDFRKSMEEMI              LSAERLLETYLV
Sbjct: 241 RGSTASAATDGGFAVVKRSSDPYEDFRKSMEEMIAEGAACGGGDGDGELSAERLLETYLV 300

Query: 301 LNSPRHYPAILAAFADVRETLFP 323
           LNSPRHYPAILAAFADVRETLFP
Sbjct: 301 LNSPRHYPAILAAFADVRETLFP 323



>Os01g0625900 Protein of unknown function DUF623, plant domain containing protein
          Length = 366

 Score = 73.9 bits (180), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 40/81 (49%), Positives = 47/81 (58%), Gaps = 6/81 (7%)

Query: 242 GSTASAATDGGFAVVKRSSDPYEDFRKSMEEMIXXXXXXXXXXXXXXLSAERLLETYLVL 301
           G+    A   G AVVKRSS+PY DFR SM EM+                 E LL +YL L
Sbjct: 289 GTDDDTAAGAGMAVVKRSSNPYADFRSSMVEMVVERRICSVP------EMEELLGSYLSL 342

Query: 302 NSPRHYPAILAAFADVRETLF 322
           NSP+H+PAILAAF DV E +F
Sbjct: 343 NSPQHHPAILAAFEDVWEAVF 363



>Os01g0226700 Protein of unknown function DUF623, plant domain containing protein
          Length = 250

 Score = 70.5 bits (171), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 39/82 (47%), Positives = 45/82 (54%), Gaps = 6/82 (7%)

Query: 240 PRGSTASAATDGGFAVVKRSSDPYEDFRKSMEEMIXXXXXXXXXXXXXXLSAERLLETYL 299
           P  S A      GFAVVKRS DPY DFR SM EM+                 ERLL +YL
Sbjct: 172 PVVSAAEEQVRRGFAVVKRSRDPYADFRSSMVEMVVGRQLFGAA------ELERLLRSYL 225

Query: 300 VLNSPRHYPAILAAFADVRETL 321
            LN+PRH+P IL AF+D+   L
Sbjct: 226 SLNAPRHHPVILQAFSDIWVVL 247



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.133    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,372,177
Number of extensions: 288689
Number of successful extensions: 1561
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1570
Number of HSP's successfully gapped: 4
Length of query: 323
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 222
Effective length of database: 11,762,187
Effective search space: 2611205514
Effective search space used: 2611205514
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)