BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0685200 Os01g0685200|J053058F10
         (120 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0685200  Anti-silencing protein, ASF1-like family protein    211   1e-55
Os05g0553800  Similar to Anti-silencing protein-like (Anti-s...   169   3e-43

>Os01g0685200 Anti-silencing protein, ASF1-like family protein
          Length = 120

 Score =  211 bits (536), Expect = 1e-55,   Method: Compositional matrix adjust.
 Identities = 104/120 (86%), Positives = 104/120 (86%)

Query: 1   YAHFRFQADPPDPSKIRKEDIIGVIVLLLTCSYMGQEFIRVGYYVNNDNDDEQLREEPPA 60
           YAHFRFQADPPDPSKIRKEDIIGVIVLLLTCSYMGQEFIRVGYYVNNDNDDEQLREEPPA
Sbjct: 1   YAHFRFQADPPDPSKIRKEDIIGVIVLLLTCSYMGQEFIRVGYYVNNDNDDEQLREEPPA 60

Query: 61  KLLIDRVQRNILADKPSVTKFPINFHPETXXXXXXXXXXXXXXXXPENHPNQGSKPNPDQ 120
           KLLIDRVQRNILADKPSVTKFPINFHPET                PENHPNQGSKPNPDQ
Sbjct: 61  KLLIDRVQRNILADKPSVTKFPINFHPETSAGAGQEQQQQQQSGSPENHPNQGSKPNPDQ 120



>Os05g0553800 Similar to Anti-silencing protein-like (Anti-silencing function 1b)
           (Anti- silencing factor 1-like protein)
          Length = 188

 Score =  169 bits (429), Expect = 3e-43,   Method: Compositional matrix adjust.
 Identities = 88/119 (73%), Positives = 91/119 (76%), Gaps = 2/119 (1%)

Query: 3   HFRFQADPPDPSKIRKEDIIGVIVLLLTCSYMGQEFIRVGYYVNNDNDDEQLREEPPAKL 62
            F  QADPPDPSKIR+EDIIGV VLLLTCSYMGQEF+RVGYYVNND DDEQLREEPPAKL
Sbjct: 71  RFVLQADPPDPSKIREEDIIGVTVLLLTCSYMGQEFMRVGYYVNNDYDDEQLREEPPAKL 130

Query: 63  LIDRVQRNILADKPSVTKFPINFHPETXXXXXXXXXXXXXXXXPENHP-NQGSKPNPDQ 120
           LIDRVQRNILADKP VTKFPINFHPE                 PENH   +GSKP  DQ
Sbjct: 131 LIDRVQRNILADKPRVTKFPINFHPE-PSTSAGQQQQEPQTASPENHTGGEGSKPAADQ 188



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.139    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,345,811
Number of extensions: 148964
Number of successful extensions: 225
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 224
Number of HSP's successfully gapped: 2
Length of query: 120
Length of database: 17,035,801
Length adjustment: 86
Effective length of query: 34
Effective length of database: 12,545,397
Effective search space: 426543498
Effective search space used: 426543498
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 149 (62.0 bits)