BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0639900 Os01g0639900|AK060890
         (272 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0639900  Similar to Carbonic anhydrase, chloroplast pre...   457   e-129
Os01g0640132  Similar to Carbonic anhydrase, chloroplast pre...   181   7e-46
Os09g0464000  Similar to Carbonate dehydratase-like protein       173   2e-43

>Os01g0639900 Similar to Carbonic anhydrase, chloroplast precursor (EC 4.2.1.1)
           (Carbonate dehydratase)
          Length = 272

 Score =  457 bits (1176), Expect = e-129,   Method: Compositional matrix adjust.
 Identities = 229/272 (84%), Positives = 229/272 (84%)

Query: 1   MSTXXXXXXXQSWCFATVTPRSRATVVAXXXXXXXXXXXXXXXXXXXXXXFRPRLIRNTP 60
           MST       QSWCFATVTPRSRATVVA                      FRPRLIRNTP
Sbjct: 1   MSTAAAAAAAQSWCFATVTPRSRATVVASLASPSPSSSSSSSNSSNLPAPFRPRLIRNTP 60

Query: 61  VFXXXXXXXXXXXXXXRLKDGFAKFKTEFYDKKPELFEPLKAGQAPKYMVFSCADSRVCP 120
           VF              RLKDGFAKFKTEFYDKKPELFEPLKAGQAPKYMVFSCADSRVCP
Sbjct: 61  VFAAPVAPAAMDAAVDRLKDGFAKFKTEFYDKKPELFEPLKAGQAPKYMVFSCADSRVCP 120

Query: 121 SVTMGLEPGEAFTVRNIANMVPAYCKIKHAGVGSAIEYAVCALKVELIVVIGHSRCGGIK 180
           SVTMGLEPGEAFTVRNIANMVPAYCKIKHAGVGSAIEYAVCALKVELIVVIGHSRCGGIK
Sbjct: 121 SVTMGLEPGEAFTVRNIANMVPAYCKIKHAGVGSAIEYAVCALKVELIVVIGHSRCGGIK 180

Query: 181 ALLSLKDGAPDSFHFVEDWVRTGFPAKKKVQTEHASLPFDDQCAILEKEAVNQSLENLKT 240
           ALLSLKDGAPDSFHFVEDWVRTGFPAKKKVQTEHASLPFDDQCAILEKEAVNQSLENLKT
Sbjct: 181 ALLSLKDGAPDSFHFVEDWVRTGFPAKKKVQTEHASLPFDDQCAILEKEAVNQSLENLKT 240

Query: 241 YPFVKEGIANGTLKLVGGHYDFVSGNLDLWEP 272
           YPFVKEGIANGTLKLVGGHYDFVSGNLDLWEP
Sbjct: 241 YPFVKEGIANGTLKLVGGHYDFVSGNLDLWEP 272



>Os01g0640132 Similar to Carbonic anhydrase, chloroplast precursor (EC 4.2.1.1)
           (Carbonate dehydratase)
          Length = 183

 Score =  181 bits (458), Expect = 7e-46,   Method: Compositional matrix adjust.
 Identities = 86/123 (69%), Positives = 100/123 (81%)

Query: 109 MVFSCADSRVCPSVTMGLEPGEAFTVRNIANMVPAYCKIKHAGVGSAIEYAVCALKVELI 168
           MVFSCADSRVCP++T GL+PGEAFTVRNIA+MVPAY K     +GSAIEYAV  LKVE I
Sbjct: 1   MVFSCADSRVCPTLTFGLQPGEAFTVRNIASMVPAYDKRGQCSIGSAIEYAVVVLKVECI 60

Query: 169 VVIGHSRCGGIKALLSLKDGAPDSFHFVEDWVRTGFPAKKKVQTEHASLPFDDQCAILEK 228
           +VIGHS CGGIK LLSLK+  P++FHFV+DWV+ G  AKKKV+ E+  LPFDDQC +LEK
Sbjct: 61  IVIGHSCCGGIKELLSLKEDRPNTFHFVDDWVKIGLAAKKKVERENMLLPFDDQCTVLEK 120

Query: 229 EAV 231
             V
Sbjct: 121 VEV 123



>Os09g0464000 Similar to Carbonate dehydratase-like protein
          Length = 306

 Score =  173 bits (438), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 89/203 (43%), Positives = 125/203 (61%), Gaps = 14/203 (6%)

Query: 80  DGFAKFKTEFYDKKPEL-------FEPLKAGQAPKYMVFSCADSRVCPSVTMGLEPGEAF 132
           D F + K  F D K          ++ L   Q PK+MV +CADSRVCPS  +G +PGEAF
Sbjct: 85  DPFMELKARFMDFKHRNCVDNISNYQNLAQQQTPKFMVVACADSRVCPSSVLGFQPGEAF 144

Query: 133 TVRNIANMVPAYCKIKHAG--VGSAIEYAVCALKVELIVVIGHSRCGGIKALLSLKDGAP 190
           TVRNIAN+VP Y   +H      +A+E+AV  L+VE ++V+GHSRCGGI+AL+S+K    
Sbjct: 145 TVRNIANLVPPY---QHGASETSAALEFAVNTLEVENVLVVGHSRCGGIQALMSMKSKQD 201

Query: 191 D--SFHFVEDWVRTGFPAKKKVQTEHASLPFDDQCAILEKEAVNQSLENLKTYPFVKEGI 248
           D  S  F+ DWV     A+   +    +L F+ QC   EKE++N SL NL TYP++++ +
Sbjct: 202 DSQSRSFIRDWVSIAKSARLSTEAAAGNLNFELQCKHCEKESINSSLLNLLTYPWIEKRV 261

Query: 249 ANGTLKLVGGHYDFVSGNLDLWE 271
             GTL L GG+Y+F+    + W+
Sbjct: 262 NEGTLSLHGGYYNFIDCTFEKWK 284



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.137    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,879,349
Number of extensions: 285930
Number of successful extensions: 472
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 469
Number of HSP's successfully gapped: 3
Length of query: 272
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 173
Effective length of database: 11,866,615
Effective search space: 2052924395
Effective search space used: 2052924395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 155 (64.3 bits)