BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0587500 Os01g0587500|Os01g0587500
         (172 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0587500  HSP20-like chaperone domain containing protein      271   1e-73
Os01g0587800                                                      108   2e-24
Os05g0592000                                                      107   6e-24
Os01g0588000  HSP20-like chaperone domain containing protein       80   7e-16

>Os01g0587500 HSP20-like chaperone domain containing protein
          Length = 172

 Score =  271 bits (694), Expect = 1e-73,   Method: Compositional matrix adjust.
 Identities = 139/172 (80%), Positives = 139/172 (80%)

Query: 1   MSTCVXXXXXXXXXXXXXXXXXXDVDLQPEVKWHDGAAGYVARLDLAGFRKEEFRVQVDG 60
           MSTCV                  DVDLQPEVKWHDGAAGYVARLDLAGFRKEEFRVQVDG
Sbjct: 1   MSTCVPKGSRSAKGAAAAEVEPRDVDLQPEVKWHDGAAGYVARLDLAGFRKEEFRVQVDG 60

Query: 61  AGRVTVRGQRPAGHVRLHREFQLPPAADVDRIAARFDGATLCLTVPKRPAGGAAEVVMAT 120
           AGRVTVRGQRPAGHVRLHREFQLPPAADVDRIAARFDGATLCLTVPKRPAGGAAEVVMAT
Sbjct: 61  AGRVTVRGQRPAGHVRLHREFQLPPAADVDRIAARFDGATLCLTVPKRPAGGAAEVVMAT 120

Query: 121 MEDARVEAEMQMEMEKERARWDRGSXXXXXXXXXXXXXXXSHRIFATRNGSA 172
           MEDARVEAEMQMEMEKERARWDRGS               SHRIFATRNGSA
Sbjct: 121 MEDARVEAEMQMEMEKERARWDRGSAIAAAVAAFALGVVVSHRIFATRNGSA 172



>Os01g0587800 
          Length = 168

 Score =  108 bits (271), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 74/128 (57%), Positives = 85/128 (66%), Gaps = 9/128 (7%)

Query: 26  DLQPEVKWHDGAA-GYVARLDLAGFRKEEFRVQVDGA-GRVTVRGQRPAG---HVRLHRE 80
           DL+ +V W DGAA GYV RLD+AGF K+E  V+V+GA GRVTV GQRPA    HVRL R 
Sbjct: 13  DLREQV-WLDGAAAGYVVRLDIAGFSKDEVDVRVNGATGRVTVLGQRPAAAGPHVRLRRV 71

Query: 81  FQLPPAADVDRIAARFDGATLCLTVP-KRPAGGAAE--VVMATMEDARVEAEMQMEMEKE 137
            QLPP AD DR+AARF G TL LTVP KRPA  AA   VVMAT  +         + ++ 
Sbjct: 72  IQLPPTADSDRVAARFVGTTLFLTVPKKRPAAAAAATGVVMATTMEVAETETETEKKKER 131

Query: 138 RARWDRGS 145
            ARWD GS
Sbjct: 132 AARWDSGS 139



>Os05g0592000 
          Length = 314

 Score =  107 bits (266), Expect = 6e-24,   Method: Compositional matrix adjust.
 Identities = 49/109 (44%), Positives = 73/109 (66%), Gaps = 4/109 (3%)

Query: 24  DVDLQPEVKWHDGAAGYVARLDLAGFRKEEFRVQVDGAGRVTVRGQRPAG----HVRLHR 79
           D +L P+ +W D A  ++ RL L GF+KE+FRVQVDG G++TVRGQRPA     + R H+
Sbjct: 24  DEELDPKFEWLDNANNFLLRLYLTGFKKEDFRVQVDGTGKLTVRGQRPAAGSKHNTRFHK 83

Query: 80  EFQLPPAADVDRIAARFDGATLCLTVPKRPAGGAAEVVMATMEDARVEA 128
            FQLP  A++D I  RF+ + L +TVPKRPA  ++     ++++ + +A
Sbjct: 84  VFQLPSNANIDDITGRFEASVLTITVPKRPAPTSSAPAPTSVQEIKQKA 132



>Os01g0588000 HSP20-like chaperone domain containing protein
          Length = 269

 Score = 80.1 bits (196), Expect = 7e-16,   Method: Compositional matrix adjust.
 Identities = 38/82 (46%), Positives = 54/82 (65%), Gaps = 2/82 (2%)

Query: 24  DVDLQPEVKWHDGAAGYVARLDLAGFRKEEFRVQVDGAGRVTVRGQRPAGHVRLHREFQL 83
           D D +P  +W D  A Y+ R+++  F+KEE +V VD AGR+TVRGQ   G +RL++ FQL
Sbjct: 10  DGDFEPVYEWLDAGAHYLLRVNVPEFKKEELQVHVDPAGRLTVRGQH--GGLRLNKVFQL 67

Query: 84  PPAADVDRIAARFDGATLCLTV 105
           PP  ++D I  R + + L LTV
Sbjct: 68  PPTCNLDAITGRLEASVLVLTV 89



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.134    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,235,762
Number of extensions: 207028
Number of successful extensions: 610
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 612
Number of HSP's successfully gapped: 4
Length of query: 172
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 78
Effective length of database: 12,127,685
Effective search space: 945959430
Effective search space used: 945959430
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 152 (63.2 bits)