BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0581300 Os01g0581300|AK066182
         (540 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0581300  Similar to Lycopene epsilon-cyclase (Fragment)      981   0.0  
Os02g0190600  Lycopene cyclase, beta and epsilon family protein   192   6e-49

>Os01g0581300 Similar to Lycopene epsilon-cyclase (Fragment)
          Length = 540

 Score =  981 bits (2535), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 481/540 (89%), Positives = 481/540 (89%)

Query: 1   MEFSGGATVSAPFGCCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWMVRCVATEKHK 60
           MEFSGGATVSAPFGCC                                WMVRCVATEKHK
Sbjct: 1   MEFSGGATVSAPFGCCRAAWGAAAAGAGAEGRSRRVVPRAVEPRRRGRWMVRCVATEKHK 60

Query: 61  DAAARRGGVEVEFADEEDYVKGGGGELLYVQMQAXXXXXXXXXXXXXLLPIPDENSVLDL 120
           DAAARRGGVEVEFADEEDYVKGGGGELLYVQMQA             LLPIPDENSVLDL
Sbjct: 61  DAAARRGGVEVEFADEEDYVKGGGGELLYVQMQASKSMDSQSKISSKLLPIPDENSVLDL 120

Query: 121 VIIGCGPAGLSLAAESAKKGLNVGLIGPDLPFTNNYGVWEDEFKDLGLESCIEHVWKDTI 180
           VIIGCGPAGLSLAAESAKKGLNVGLIGPDLPFTNNYGVWEDEFKDLGLESCIEHVWKDTI
Sbjct: 121 VIIGCGPAGLSLAAESAKKGLNVGLIGPDLPFTNNYGVWEDEFKDLGLESCIEHVWKDTI 180

Query: 181 VYLDGNKPIMIGRAYGXXXXXXXXXXXXXXCYDAGVTYLSSKVDKIMESPDGHRVVCCEG 240
           VYLDGNKPIMIGRAYG              CYDAGVTYLSSKVDKIMESPDGHRVVCCEG
Sbjct: 181 VYLDGNKPIMIGRAYGRVHRDLLHEELLRRCYDAGVTYLSSKVDKIMESPDGHRVVCCEG 240

Query: 241 DREVLCRLAIVASGAASGRLLEYEVGGPRVCVQTAYGVEVEVENNPYDPSLMVFMDYRDC 300
           DREVLCRLAIVASGAASGRLLEYEVGGPRVCVQTAYGVEVEVENNPYDPSLMVFMDYRDC
Sbjct: 241 DREVLCRLAIVASGAASGRLLEYEVGGPRVCVQTAYGVEVEVENNPYDPSLMVFMDYRDC 300

Query: 301 FKDKFSHPEQGNPTFLYAMPMSSTRIFFEETCLASKEAMPFDLLKKRLMSRLDAMGVHIR 360
           FKDKFSHPEQGNPTFLYAMPMSSTRIFFEETCLASKEAMPFDLLKKRLMSRLDAMGVHIR
Sbjct: 301 FKDKFSHPEQGNPTFLYAMPMSSTRIFFEETCLASKEAMPFDLLKKRLMSRLDAMGVHIR 360

Query: 361 KVYEEEWSYIPVGGSLPNTDQKNLAFGAAASMVHPATGYSVVRSLSEAPRYASVISDILR 420
           KVYEEEWSYIPVGGSLPNTDQKNLAFGAAASMVHPATGYSVVRSLSEAPRYASVISDILR
Sbjct: 361 KVYEEEWSYIPVGGSLPNTDQKNLAFGAAASMVHPATGYSVVRSLSEAPRYASVISDILR 420

Query: 421 NRVYPGEYLPGTSQSSSPSMLAWRTLWPQERKRQRSFFLFGLALIIQLNNEGIQTFFETF 480
           NRVYPGEYLPGTSQSSSPSMLAWRTLWPQERKRQRSFFLFGLALIIQLNNEGIQTFFETF
Sbjct: 421 NRVYPGEYLPGTSQSSSPSMLAWRTLWPQERKRQRSFFLFGLALIIQLNNEGIQTFFETF 480

Query: 481 FRLPKWMWRGFLGSTLSSVDLILFAFYMFTIAPNQMRMNLVRHLLSDPTGSTMIKTYLTL 540
           FRLPKWMWRGFLGSTLSSVDLILFAFYMFTIAPNQMRMNLVRHLLSDPTGSTMIKTYLTL
Sbjct: 481 FRLPKWMWRGFLGSTLSSVDLILFAFYMFTIAPNQMRMNLVRHLLSDPTGSTMIKTYLTL 540



>Os02g0190600 Lycopene cyclase, beta and epsilon family protein
          Length = 267

 Score =  192 bits (487), Expect = 6e-49,   Method: Compositional matrix adjust.
 Identities = 101/256 (39%), Positives = 147/256 (57%), Gaps = 16/256 (6%)

Query: 273 QTAYGVEVEVENNPYDPSLMVFMDYRDCFKDKFSHPEQGN---PTFLYAMPMSSTRIFFE 329
           Q AYG+  EV+ +P+D   M+FMD+RD    + S   + N   PTFLYAMP S TRIF E
Sbjct: 1   QVAYGILAEVDGHPFDIDKMLFMDWRDAHLPEGSEIRERNRRIPTFLYAMPFSPTRIFLE 60

Query: 330 ETCLASKEAMPFDLLKKRLMSRLDAMGVHIRKVYEEEWSYIPVGGSLPNTDQKNLAFGAA 389
           ET L ++  +  D +++R+ +RL  +G+ +R V E+E   IP+GG LP   Q+ +  G  
Sbjct: 61  ETSLVARPGLAMDDIQERMAARLRHLGIRVRAVEEDERCVIPMGGPLPVLPQRVVGIGGT 120

Query: 390 ASMVHPATGYSVVRSLSEAPRYASVISDILR----NRVYPGEYLPGTSQSSSPSMLAWRT 445
           A MVHP+TGY V R+L+ AP  A  I   L     +  + G+ L         S   WR 
Sbjct: 121 AGMVHPSTGYMVARTLATAPIVADAIVRFLDTGSGDSAFAGDAL---------SAEVWRE 171

Query: 446 LWPQERKRQRSFFLFGLALIIQLNNEGIQTFFETFFRLPKWMWRGFLGSTLSSVDLILFA 505
           LWP +R+RQR FF FG+ ++++L+ +G + FF+ FF L    W GFL S L   +L +F 
Sbjct: 172 LWPAQRRRQREFFCFGMDILLKLDLDGTRRFFDAFFDLEPRYWHGFLSSRLFLPELAMFG 231

Query: 506 FYMFTIAPNQMRMNLV 521
             +F  A N  R+ ++
Sbjct: 232 LSLFAKASNTSRLEIM 247



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.138    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 17,584,224
Number of extensions: 708617
Number of successful extensions: 1592
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1589
Number of HSP's successfully gapped: 2
Length of query: 540
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 434
Effective length of database: 11,501,117
Effective search space: 4991484778
Effective search space used: 4991484778
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 158 (65.5 bits)