BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0253800 Os01g0253800|009-121-G03
(175 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os01g0253800 Conserved hypothetical protein 281 2e-76
Os03g0304100 Conserved hypothetical protein 92 2e-19
Os05g0355900 Conserved hypothetical protein 87 9e-18
>Os01g0253800 Conserved hypothetical protein
Length = 175
Score = 281 bits (719), Expect = 2e-76, Method: Compositional matrix adjust.
Identities = 138/138 (100%), Positives = 138/138 (100%)
Query: 1 MAWVSMTWCWHRSFLLMMACVCSWSITCCKSDEAAAGAFHGQKRSTEDIVARAIICFSDR 60
MAWVSMTWCWHRSFLLMMACVCSWSITCCKSDEAAAGAFHGQKRSTEDIVARAIICFSDR
Sbjct: 1 MAWVSMTWCWHRSFLLMMACVCSWSITCCKSDEAAAGAFHGQKRSTEDIVARAIICFSDR 60
Query: 61 YIFSGCQGQYRLGPEGALRVPAAAAEAFCGGPCLAETRLVLGCVDGIMDSFRFYNGASVR 120
YIFSGCQGQYRLGPEGALRVPAAAAEAFCGGPCLAETRLVLGCVDGIMDSFRFYNGASVR
Sbjct: 61 YIFSGCQGQYRLGPEGALRVPAAAAEAFCGGPCLAETRLVLGCVDGIMDSFRFYNGASVR 120
Query: 121 DVRLALDRGCGLRGDFDV 138
DVRLALDRGCGLRGDFDV
Sbjct: 121 DVRLALDRGCGLRGDFDV 138
>Os03g0304100 Conserved hypothetical protein
Length = 180
Score = 92.0 bits (227), Expect = 2e-19, Method: Compositional matrix adjust.
Identities = 44/92 (47%), Positives = 59/92 (64%), Gaps = 3/92 (3%)
Query: 49 IVARAIICFSDRYIFSGCQGQYRLGPEGALRVPAAAAEAFCGGPCLAETRLVLGCVDGIM 108
+ +R++ CF D ++S C+G YRL P G + VP +++CGG C+ ET VL CV +
Sbjct: 41 LPSRSLRCFDDGQVYSCCEGAYRLNPSGVIAVPPGEVDSYCGGACVVETEDVLNCVASAL 100
Query: 109 DSFRFYNGASVRDVRLALDRGCG---LRGDFD 137
D FRF+NGASV D R AL RGC RGDF+
Sbjct: 101 DGFRFFNGASVEDARYALRRGCSHTIKRGDFN 132
>Os05g0355900 Conserved hypothetical protein
Length = 209
Score = 86.7 bits (213), Expect = 9e-18, Method: Compositional matrix adjust.
Identities = 50/131 (38%), Positives = 69/131 (52%), Gaps = 15/131 (11%)
Query: 12 RSFLLMMACVCSWSITCCKSDEAAAGAFHGQKRSTEDIVARAIICFSDRYIFSGCQGQYR 71
RS L A + CC +AA F +A CF D ++S C+ + R
Sbjct: 10 RSVCLAFAIAVCLLLPCCFGSKAAVEMFE-----------KACHCFDDHNVYSECKEELR 58
Query: 72 LGPEGALRVPAAAAEAFCGGPCLAETRLVLGCVDGI-MDSFRFYNGASVRDVRLALDRGC 130
LG EGA V + + +CGGPCL ET++ L CV+ + D FRFY+GAS+ V+ ALD GC
Sbjct: 59 LGVEGAFHVGKESVDEYCGGPCLMETKMALQCVEEVAHDGFRFYSGASLPAVKAALDTGC 118
Query: 131 GL---RGDFDV 138
RG F++
Sbjct: 119 SYTPDRGTFEI 129
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.329 0.140 0.488
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,159,022
Number of extensions: 198378
Number of successful extensions: 565
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 565
Number of HSP's successfully gapped: 3
Length of query: 175
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 81
Effective length of database: 12,127,685
Effective search space: 982342485
Effective search space used: 982342485
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 152 (63.2 bits)