BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0250300 Os01g0250300|AK106665
         (122 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0250300  Hypothetical protein                                229   4e-61
Os01g0244200  Triosephosphate isomerase family protein             85   1e-17
Os10g0502800                                                       84   2e-17

>Os01g0250300 Hypothetical protein
          Length = 122

 Score =  229 bits (583), Expect = 4e-61,   Method: Compositional matrix adjust.
 Identities = 109/122 (89%), Positives = 109/122 (89%)

Query: 1   MAAVGVSFVSLLNAVAWHCRLPSVSYLWELDGNDALPSDESPSKTAKYGFAVHDYNLQTL 60
           MAAVGVSFVSLLNAVAWHCRLPSVSYLWELDGNDALPSDESPSKTAKYGFAVHDYNLQTL
Sbjct: 1   MAAVGVSFVSLLNAVAWHCRLPSVSYLWELDGNDALPSDESPSKTAKYGFAVHDYNLQTL 60

Query: 61  LSYRQIXXXXXXXXXXXXXHLARFRARYGPMDLSCECILRQCIALWSNFVKEDHFFYSTY 120
           LSYRQI             HLARFRARYGPMDLSCECILRQCIALWSNFVKEDHFFYSTY
Sbjct: 61  LSYRQIAASAVDAALTASTHLARFRARYGPMDLSCECILRQCIALWSNFVKEDHFFYSTY 120

Query: 121 RP 122
           RP
Sbjct: 121 RP 122



>Os01g0244200 Triosephosphate isomerase family protein
          Length = 2498

 Score = 84.7 bits (208), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 49/103 (47%), Positives = 56/103 (54%), Gaps = 37/103 (35%)

Query: 1   MAAVGVSFVSLLNAVAWHCRLPSVSYLWELDGND--------ALPSDESPSK-------- 44
           M  VGVSFVSLLNAVAWHC+LP VSYLWE+DGN         ALPS++   +        
Sbjct: 1   MVNVGVSFVSLLNAVAWHCQLPLVSYLWEIDGNGMLLAGVELALPSEDMAGQIVSKFFWV 60

Query: 45  ---------------------TAKYGFAVHDYNLQTLLSYRQI 66
                                 A+YGF VHDYNLQ LLSYR+I
Sbjct: 61  CASTQTSDSHEMGAHQAVRFLQARYGFIVHDYNLQMLLSYRRI 103



>Os10g0502800 
          Length = 1077

 Score = 84.3 bits (207), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 49/103 (47%), Positives = 56/103 (54%), Gaps = 37/103 (35%)

Query: 1   MAAVGVSFVSLLNAVAWHCRLPSVSYLWELDGND--------ALPSDESPSK-------- 44
           M  VGVSFVSLLNAVAWHC+LP VSYLWE+DGN         ALPS++   +        
Sbjct: 1   MVNVGVSFVSLLNAVAWHCQLPLVSYLWEIDGNGMLLAGVELALPSEDMAGQIVSKFFWV 60

Query: 45  ---------------------TAKYGFAVHDYNLQTLLSYRQI 66
                                 A+YGF VHDYNLQ LLSYR+I
Sbjct: 61  CASTQTSDSHEMVAHQAVRFLQARYGFIVHDYNLQMLLSYRRI 103



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.326    0.137    0.450 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,000,816
Number of extensions: 127725
Number of successful extensions: 341
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 343
Number of HSP's successfully gapped: 3
Length of query: 122
Length of database: 17,035,801
Length adjustment: 88
Effective length of query: 34
Effective length of database: 12,440,969
Effective search space: 422992946
Effective search space used: 422992946
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 149 (62.0 bits)