BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0217100 Os01g0217100|AK099424
         (183 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0217100  Conserved hypothetical protein                      283   5e-77
Os04g0587400  Protein of unknown function DUF250 domain cont...    88   4e-18

>Os01g0217100 Conserved hypothetical protein
          Length = 183

 Score =  283 bits (724), Expect = 5e-77,   Method: Compositional matrix adjust.
 Identities = 148/183 (80%), Positives = 148/183 (80%)

Query: 1   MSLMRPRSSNASSTPVAAKKEKTATRWCSQSAQEKXXXXXXXXXXXXXXXXAESDKGTSR 60
           MSLMRPRSSNASSTPVAAKKEKTATRWCSQSAQEK                AESDKGTSR
Sbjct: 1   MSLMRPRSSNASSTPVAAKKEKTATRWCSQSAQEKVVVVRVQGVRGQEDVVAESDKGTSR 60

Query: 61  XXXXXXXXXXXXXVSSKRLRWWAVVLANIVFVLGGQSVGCFSAGYTTIRGGDSLWLATVV 120
                        VSSKRLRWWAVVLANIVFVLGGQSVGCFSAGYTTIRGGDSLWLATVV
Sbjct: 61  ADATAAAAAAPPPVSSKRLRWWAVVLANIVFVLGGQSVGCFSAGYTTIRGGDSLWLATVV 120

Query: 121 QSCGAPLTVXXXXXXYFQMGSPAPPLIEPSERWWRQSSAGAGLDGRLSPSAAGSVEKRGE 180
           QSCGAPLTV      YFQMGSPAPPLIEPSERWWRQSSAGAGLDGRLSPSAAGSVEKRGE
Sbjct: 121 QSCGAPLTVLLLLLLYFQMGSPAPPLIEPSERWWRQSSAGAGLDGRLSPSAAGSVEKRGE 180

Query: 181 EKK 183
           EKK
Sbjct: 181 EKK 183



>Os04g0587400 Protein of unknown function DUF250 domain containing protein
          Length = 408

 Score = 87.8 bits (216), Expect = 4e-18,   Method: Compositional matrix adjust.
 Identities = 57/104 (54%), Positives = 62/104 (59%), Gaps = 9/104 (8%)

Query: 52  AESDKGTSRXXXXXXXXXXXXX---VSSKRLRWWAVVLANIVFVLGGQSVGCFSAGYTTI 108
           AESDK TSR                VSS+RLRWWAVVLANIVFVLGGQSV          
Sbjct: 39  AESDKSTSRADGTAAAAAAAAPLPPVSSQRLRWWAVVLANIVFVLGGQSVATLLGRIYYD 98

Query: 109 RGGDSLWLATVVQSCGAPLTVXXXXXXYFQM--GSPA--PPLIE 148
           +GG SLWLATVVQSCGAPL V      YF+    SP   PPL++
Sbjct: 99  QGGGSLWLATVVQSCGAPLAV--PLLLYFRRPEASPVARPPLLK 140



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.125    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,552,253
Number of extensions: 204773
Number of successful extensions: 537
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 536
Number of HSP's successfully gapped: 2
Length of query: 183
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 88
Effective length of database: 12,075,471
Effective search space: 1062641448
Effective search space used: 1062641448
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 153 (63.5 bits)