BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0636600 Os12g0636600|AK111056
         (187 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0636600  Conserved hypothetical protein                      274   3e-74
Os04g0681700                                                       91   4e-19
Os04g0681800  Conserved hypothetical protein                       90   1e-18

>Os12g0636600 Conserved hypothetical protein
          Length = 187

 Score =  274 bits (700), Expect = 3e-74,   Method: Compositional matrix adjust.
 Identities = 140/187 (74%), Positives = 140/187 (74%)

Query: 1   MADGDRQEAEAPVAGIKRVKEEEATASPSGGEKKKILPNWRKSSIPCEGSEILXXXXEAI 60
           MADGDRQEAEAPVAGIKRVKEEEATASPSGGEKKKILPNWRKSSIPCEGSEIL    EAI
Sbjct: 1   MADGDRQEAEAPVAGIKRVKEEEATASPSGGEKKKILPNWRKSSIPCEGSEILKKKKEAI 60

Query: 61  AARPLWVSRDVPGAMECWIXXXXXXXXXXXXDIASGXXXXXXXXXXXMPNEVIQQMMRXX 120
           AARPLWVSRDVPGAMECWI            DIASG           MPNEVIQQMMR  
Sbjct: 61  AARPLWVSRDVPGAMECWIEEREKALAEEEADIASGKKKRKKVVKYKMPNEVIQQMMRYP 120

Query: 121 XXXXXXXXXXLARRSASNRQLHRLRMFIDGKMFAYEQTLIDQYLKHGYAFDEAEISDXXX 180
                     LARRSASNRQLHRLRMFIDGKMFAYEQTLIDQYLKHGYAFDEAEISD   
Sbjct: 121 YTYPECTEEELARRSASNRQLHRLRMFIDGKMFAYEQTLIDQYLKHGYAFDEAEISDEEE 180

Query: 181 XXXXXQK 187
                QK
Sbjct: 181 EEEEEQK 187



>Os04g0681700 
          Length = 197

 Score = 90.9 bits (224), Expect = 4e-19,   Method: Compositional matrix adjust.
 Identities = 67/192 (34%), Positives = 88/192 (45%), Gaps = 30/192 (15%)

Query: 1   MADGDRQEAEAPVAGIKRVK------EEEATASPSGGEKKKILPNWRKSSIPCEGSEILX 54
           M DG+R  AE   AG KR +       E A AS    +K+KILP WR ++IPCEG E+L 
Sbjct: 1   MRDGER--AEVGAAGKKRKEVSASSDSEPAAASGKKTKKEKILPAWRANAIPCEGGEVLR 58

Query: 55  XXXEAIAARPLWVSRDVPGAM---------------ECWIXXXXXXXXXXXXDIASGXXX 99
              EA+AA  LW + D+PGAM               E W+                    
Sbjct: 59  RKKEAVAAGKLWSTPDIPGAMGSHLWDPIEVPAEMLELWLERQKANAEAAAAKKKK---- 114

Query: 100 XXXXXXXXMPNEVIQQMMRX--XXXXXXXXXXXLARRSASNRQLHRLRMFIDGKMFAYEQ 157
                   +PN +++ M+               LA  + S+RQ + LR FID K   YEQ
Sbjct: 115 -RKVFKCRVPNSLVEVMIARPYKCVDHDRSQEELAELTVSHRQGYILRKFIDEKKMKYEQ 173

Query: 158 TLIDQYLKHGYA 169
           TLID+Y+K GYA
Sbjct: 174 TLIDRYVKQGYA 185



>Os04g0681800 Conserved hypothetical protein
          Length = 218

 Score = 89.7 bits (221), Expect = 1e-18,   Method: Compositional matrix adjust.
 Identities = 68/183 (37%), Positives = 90/183 (49%), Gaps = 19/183 (10%)

Query: 1   MADGDRQEAEAPVAGIKRVKEEEATAS----PSGGEKKK-ILPNWRKSSIPCEGSEILXX 55
           M DG+R  AE   AG+KR   +EA+AS    P+G + KK ILP WR ++IPCEG E+L  
Sbjct: 22  MRDGER--AEVGAAGVKR---KEASASSDSEPAGKKAKKKILPKWRANAIPCEGGEVLRR 76

Query: 56  XXEAIAARPLWVSRDVPGAMECWIXXXXXXXX-------XXXXDIASGXXXXXXXXXXXM 108
             EA+AA  LW + DVPGA + W                      A+            +
Sbjct: 77  KKEAVAASMLWRTPDVPGASDVWDSIEVPAEMLELWLERQKAKAEAAAAKKKRKVFKCRV 136

Query: 109 PNEVIQQMMRX--XXXXXXXXXXXLARRSASNRQLHRLRMFIDGKMFAYEQTLIDQYLKH 166
           PN +++ M+               LA  +  +RQ++ LR FID K   YEQTLIDQY   
Sbjct: 137 PNSLVEVMITQPYKCVDHNRSQEELAELAVPHRQIYILRKFIDEKKMNYEQTLIDQYATQ 196

Query: 167 GYA 169
           GYA
Sbjct: 197 GYA 199



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.131    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,731,612
Number of extensions: 151324
Number of successful extensions: 424
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 423
Number of HSP's successfully gapped: 3
Length of query: 187
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 92
Effective length of database: 12,075,471
Effective search space: 1110943332
Effective search space used: 1110943332
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 153 (63.5 bits)