BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0616200 Os12g0616200|AK059735
         (170 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0616200  HUELLENLOS-like protein                             313   3e-86
Os05g0292700  Chloroplast 50S ribosomal protein L14                83   1e-16

>Os12g0616200 HUELLENLOS-like protein
          Length = 170

 Score =  313 bits (802), Expect = 3e-86,   Method: Compositional matrix adjust.
 Identities = 156/170 (91%), Positives = 156/170 (91%)

Query: 1   MAAFLRSKCSSVGRTLMGGLGNNLFGAVNSSVETVSRPSHCDPIFQQIRTFIQMRTNLKV 60
           MAAFLRSKCSSVGRTLMGGLGNNLFGAVNSSVETVSRPSHCDPIFQQIRTFIQMRTNLKV
Sbjct: 1   MAAFLRSKCSSVGRTLMGGLGNNLFGAVNSSVETVSRPSHCDPIFQQIRTFIQMRTNLKV 60

Query: 61  VDNSGAKRVMCIQSLRGKKGARLGDTIIGSVKEAQPRXXXXXXXXXXXXXXRAAMKRGRN 120
           VDNSGAKRVMCIQSLRGKKGARLGDTIIGSVKEAQPR              RAAMKRGRN
Sbjct: 61  VDNSGAKRVMCIQSLRGKKGARLGDTIIGSVKEAQPRGKVKKGDVVYGVVVRAAMKRGRN 120

Query: 121 DGSEIQFDDNAIVLVNNKGELIGTRVFGPVPHELRKKKHLKILALAEHIV 170
           DGSEIQFDDNAIVLVNNKGELIGTRVFGPVPHELRKKKHLKILALAEHIV
Sbjct: 121 DGSEIQFDDNAIVLVNNKGELIGTRVFGPVPHELRKKKHLKILALAEHIV 170



>Os05g0292700 Chloroplast 50S ribosomal protein L14
          Length = 123

 Score = 82.8 bits (203), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 45/123 (36%), Positives = 70/123 (56%), Gaps = 3/123 (2%)

Query: 51  FIQMRTNLKVVDNSGAKRVMCIQSL---RGKKGARLGDTIIGSVKEAQPRXXXXXXXXXX 107
            IQ +T L V DNSGA+++MCI+ +     ++ AR+GD I+  +K+A P+          
Sbjct: 1   MIQPQTLLNVADNSGARKLMCIRVIGAASNQRYARIGDVIVAVIKDAVPQMPLERSEVIR 60

Query: 108 XXXXRAAMKRGRNDGSEIQFDDNAIVLVNNKGELIGTRVFGPVPHELRKKKHLKILALAE 167
               R   +    DG  I++DDNA V+++ KG   GTRVFG +  ELR+    KI++LA 
Sbjct: 61  AVIVRTCKEFKCEDGIIIRYDDNAAVIIDQKGNPKGTRVFGAIAEELRELNFTKIVSLAP 120

Query: 168 HIV 170
            ++
Sbjct: 121 EVL 123



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.136    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,838,969
Number of extensions: 157003
Number of successful extensions: 352
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 354
Number of HSP's successfully gapped: 2
Length of query: 170
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 76
Effective length of database: 12,127,685
Effective search space: 921704060
Effective search space used: 921704060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 152 (63.2 bits)