BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0563400 Os12g0563400|AK067785
         (625 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0563400  Rhodanese-like domain containing protein           1256   0.0  
Os05g0323100  Rhodanese-like domain containing protein            118   1e-26
Os03g0861700  Rhodanese-like domain containing protein             77   3e-14

>Os12g0563400 Rhodanese-like domain containing protein
          Length = 625

 Score = 1256 bits (3250), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 607/625 (97%), Positives = 607/625 (97%)

Query: 1   MDAAAPPPRXXXXXXXXXXXXXXXXXXVLLYYKYAEVPDAAALAAFYESHCRALALVGRV 60
           MDAAAPPPR                  VLLYYKYAEVPDAAALAAFYESHCRALALVGRV
Sbjct: 1   MDAAAPPPRGQEGQGDRSGGDEGGRYGVLLYYKYAEVPDAAALAAFYESHCRALALVGRV 60

Query: 61  RVGPDGVNATLGGRMAALEKHVAEMSSNALFDGTDFKLASCDDPVDERVARECGFTSLSV 120
           RVGPDGVNATLGGRMAALEKHVAEMSSNALFDGTDFKLASCDDPVDERVARECGFTSLSV
Sbjct: 61  RVGPDGVNATLGGRMAALEKHVAEMSSNALFDGTDFKLASCDDPVDERVARECGFTSLSV 120

Query: 121 RLVKELVTLCANPSLATPQITCAGRHLSAAEFHSVLQSVAGATSDSEATVEKSEVVVLDA 180
           RLVKELVTLCANPSLATPQITCAGRHLSAAEFHSVLQSVAGATSDSEATVEKSEVVVLDA
Sbjct: 121 RLVKELVTLCANPSLATPQITCAGRHLSAAEFHSVLQSVAGATSDSEATVEKSEVVVLDA 180

Query: 181 RNVYETRIGKFRVPNVETLDPEIRQYSDLPLWIDEHAEKLRGKSIMMYCTGGIRCEMASA 240
           RNVYETRIGKFRVPNVETLDPEIRQYSDLPLWIDEHAEKLRGKSIMMYCTGGIRCEMASA
Sbjct: 181 RNVYETRIGKFRVPNVETLDPEIRQYSDLPLWIDEHAEKLRGKSIMMYCTGGIRCEMASA 240

Query: 241 YIRSKGEGFENVFQLYGGIQRYLERFPDGGYFEGKNFVFDHRISVGSLKENILGTCLLCG 300
           YIRSKGEGFENVFQLYGGIQRYLERFPDGGYFEGKNFVFDHRISVGSLKENILGTCLLCG
Sbjct: 241 YIRSKGEGFENVFQLYGGIQRYLERFPDGGYFEGKNFVFDHRISVGSLKENILGTCLLCG 300

Query: 301 SSFDDYSPRCRCSHCRMLVLVCSTCQDSTKDYVCELCQKNGKQCCQTSPRQGCKTESELI 360
           SSFDDYSPRCRCSHCRMLVLVCSTCQDSTKDYVCELCQKNGKQCCQTSPRQGCKTESELI
Sbjct: 301 SSFDDYSPRCRCSHCRMLVLVCSTCQDSTKDYVCELCQKNGKQCCQTSPRQGCKTESELI 360

Query: 361 DSSDFGIPMIINQSATSTIPRSNGSEQLKKLKILCLHGFRQNASNFKGRTSALAKKLKHI 420
           DSSDFGIPMIINQSATSTIPRSNGSEQLKKLKILCLHGFRQNASNFKGRTSALAKKLKHI
Sbjct: 361 DSSDFGIPMIINQSATSTIPRSNGSEQLKKLKILCLHGFRQNASNFKGRTSALAKKLKHI 420

Query: 421 ADLVFIDAPHELSFVYKPNPDHCSGRSSLPSGTPKRKYAWLVAPNSIFYAEHDWKIADAP 480
           ADLVFIDAPHELSFVYKPNPDHCSGRSSLPSGTPKRKYAWLVAPNSIFYAEHDWKIADAP
Sbjct: 421 ADLVFIDAPHELSFVYKPNPDHCSGRSSLPSGTPKRKYAWLVAPNSIFYAEHDWKIADAP 480

Query: 481 FDPLQYQQQTDGFEESYAYLEHAISQMGNIDGILGFSQGAAMAALFCRQQQKTCGSLKFR 540
           FDPLQYQQQTDGFEESYAYLEHAISQMGNIDGILGFSQGAAMAALFCRQQQKTCGSLKFR
Sbjct: 481 FDPLQYQQQTDGFEESYAYLEHAISQMGNIDGILGFSQGAAMAALFCRQQQKTCGSLKFR 540

Query: 541 FGIFCSGYPAPIISDFDGEPIKLPSLHCFGNSEDHDRQIANRASTELANRFDKSCRSVIE 600
           FGIFCSGYPAPIISDFDGEPIKLPSLHCFGNSEDHDRQIANRASTELANRFDKSCRSVIE
Sbjct: 541 FGIFCSGYPAPIISDFDGEPIKLPSLHCFGNSEDHDRQIANRASTELANRFDKSCRSVIE 600

Query: 601 HDMGHIIPTRPPFIDKIKEFLSNFI 625
           HDMGHIIPTRPPFIDKIKEFLSNFI
Sbjct: 601 HDMGHIIPTRPPFIDKIKEFLSNFI 625



>Os05g0323100 Rhodanese-like domain containing protein
          Length = 462

 Score =  118 bits (295), Expect = 1e-26,   Method: Compositional matrix adjust.
 Identities = 92/324 (28%), Positives = 143/324 (44%), Gaps = 72/324 (22%)

Query: 28  VLLYYKYAEVPDAAALAAFYESHCRALALVGRVRVGPDGVNATLGGRMAALEKHVAEMSS 87
           V+ +Y++A+ PD A L    +  C  L + G + + P+G+N ++ G   A+EK +  + S
Sbjct: 95  VVSFYRFADFPDHAELRWPLKELCEELRVSGGIILAPEGINGSICGTPEAVEKVLNFIQS 154

Query: 88  NALFDGTDFKLASCDDPVDERVARECGFTSLS----------------VRLVKELVTLCA 131
           +    G    + S   P DE +    G TS S                V+L KE+V L  
Sbjct: 155 DDRLKGLRM-IQSPVTPEDEAIHH--GHTSQSPVGAGEDAPFRWDHVRVKLKKEIVALGD 211

Query: 132 NPSLATPQITCAGRHLSAAEFHSVLQSVAGATSDSEATVEKSEVVVLDARNVYETRIGKF 191
              + T  +   G+++   +++              A +   + VV+D RN+YE RIGKF
Sbjct: 212 PGVMPTKTV---GKYVKPKDWN--------------ALISDPDTVVIDVRNMYEIRIGKF 254

Query: 192 RVPNVETLDPEIRQYSDLPLWIDEHAEKLRGKS--------------------------- 224
           +      +DP    + + P W+D+  +     S                           
Sbjct: 255 K----RAVDPCTNSFREFPSWVDDQFQLAESDSQLCSENNDNTIGRQSEDVNSSKPKELP 310

Query: 225 -IMMYCTGGIRCEMASAYIRSKGEGFENVFQLYGGIQRYLERFPDG-GYFEGKNFVFDHR 282
            + MYCTGGIRCE AS+++ SK  GF+ V+ L GGI +YLE  P     +EG+ FVFD R
Sbjct: 311 RVAMYCTGGIRCEKASSFLLSK--GFKEVYHLEGGILKYLEEIPKAESLWEGECFVFDKR 368

Query: 283 ISVG-SLKENILGTCLLCGSSFDD 305
           +SV   L +     C  C     D
Sbjct: 369 VSVEHGLAQGTHKLCYGCKKPVSD 392



>Os03g0861700 Rhodanese-like domain containing protein
          Length = 405

 Score = 77.4 bits (189), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 89/344 (25%), Positives = 138/344 (40%), Gaps = 68/344 (19%)

Query: 28  VLLYYKYAEVPDAAALAAFYESHCRALALVGRVRVGPDGVNATLGGRMAALEKHVAEMSS 87
           V+ +YK+  + D  A  + +    +   + GR+ +   G+NA   G       +   +  
Sbjct: 62  VVTFYKFVSIDDPRAEVSRHLHFLQGRDIHGRIYMNEQGINAQYSGPHKDAVAYADWLRK 121

Query: 88  NALFDGTDFKLASCDDPVDERVARECG--FTSLSVRLVKELVTLCANPSLATPQITCAGR 145
           +  F           D + +     CG  F  L +R+             ATP       
Sbjct: 122 DHRFR----------DLLVQTSPSLCGHAFPRLKLRM------------RATP------- 152

Query: 146 HLSAAEFHSVLQSVAGA-TSDSEATVEKS--EVVVLDARNVYETRIGKFR---VPNVETL 199
            L+ +E+   L++      S SEA  + S   +++LD RN YE  IG F+    PNV+  
Sbjct: 153 -LTPSEWRERLEARKCLDVSSSEAAGDSSGRRLLLLDVRNDYEWDIGHFQGAQRPNVDCF 211

Query: 200 DPEIRQYSDLPLWIDEHAEKLRG-----KSIMMYCTGGIRCEMASAYIRSKGEGFENVFQ 254
                  S+    +D  ++ L G       I+MYCTGGIRC++ S  +R K  GF N++ 
Sbjct: 212 RSTSFGLSESEQEMDS-SDPLNGIDKENTDILMYCTGGIRCDVYSTILRKK--GFRNLYT 268

Query: 255 LYGGIQRYLERFPDGGYFEGKNFVFDHRISV--------------------GSLKENILG 294
           L GG+  YL+     G+  G  FVFD R+S+                     +   + LG
Sbjct: 269 LKGGVSNYLKEEGSAGWV-GNLFVFDGRLSLPPATYKPGAGDDDDEEEEEGRNRSSSELG 327

Query: 295 TCLLCGSSFDDYSPR-CRCSHCRMLVLVCSTCQDSTKDYVCELC 337
            C  CGS   +   R C    C  L L C  C +  +    E C
Sbjct: 328 RCYACGSEVVELRHRNCANIDCNRLYLCCGRCVEELRGCCGEGC 371



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.136    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 22,170,310
Number of extensions: 955001
Number of successful extensions: 2208
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 2206
Number of HSP's successfully gapped: 3
Length of query: 625
Length of database: 17,035,801
Length adjustment: 107
Effective length of query: 518
Effective length of database: 11,448,903
Effective search space: 5930531754
Effective search space used: 5930531754
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 159 (65.9 bits)