BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0535400 Os12g0535400|AK065318
         (161 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0535400  Hypothetical protein                                328   8e-91
Os03g0288300  Esterase/lipase/thioesterase domain containing...   239   4e-64
Os12g0250850                                                      123   6e-29
Os07g0289600  Hypothetical protein                                 89   1e-18

>Os12g0535400 Hypothetical protein
          Length = 161

 Score =  328 bits (842), Expect = 8e-91,   Method: Compositional matrix adjust.
 Identities = 161/161 (100%), Positives = 161/161 (100%)

Query: 1   CQIHWQAVDPAFGLPPVDPTAQSLAGAEDSELARRLRGPAVAMGKRLSFMNAYLAEDCNP 60
           CQIHWQAVDPAFGLPPVDPTAQSLAGAEDSELARRLRGPAVAMGKRLSFMNAYLAEDCNP
Sbjct: 1   CQIHWQAVDPAFGLPPVDPTAQSLAGAEDSELARRLRGPAVAMGKRLSFMNAYLAEDCNP 60

Query: 61  VRCWVITAAVAFVTLIVLGVGSVDDTPVELPKKLYIGPPSAKTIQLPDGRHLAYKEQGVT 120
           VRCWVITAAVAFVTLIVLGVGSVDDTPVELPKKLYIGPPSAKTIQLPDGRHLAYKEQGVT
Sbjct: 61  VRCWVITAAVAFVTLIVLGVGSVDDTPVELPKKLYIGPPSAKTIQLPDGRHLAYKEQGVT 120

Query: 121 ADRARFSLIAPHYFLSSRLAGIPGIKPSLLEKFGARLVIIN 161
           ADRARFSLIAPHYFLSSRLAGIPGIKPSLLEKFGARLVIIN
Sbjct: 121 ADRARFSLIAPHYFLSSRLAGIPGIKPSLLEKFGARLVIIN 161



>Os03g0288300 Esterase/lipase/thioesterase domain containing protein
          Length = 536

 Score =  239 bits (611), Expect = 4e-64,   Method: Compositional matrix adjust.
 Identities = 128/144 (88%), Positives = 132/144 (91%)

Query: 18  DPTAQSLAGAEDSELARRLRGPAVAMGKRLSFMNAYLAEDCNPVRCWVITAAVAFVTLIV 77
           D  AQSLAGAEDSELARRLRGPA A GKRLSFMN YL ED +PV CWV+ AAVAFVTLIV
Sbjct: 85  DIAAQSLAGAEDSELARRLRGPAAAAGKRLSFMNEYLPEDRDPVWCWVVVAAVAFVTLIV 144

Query: 78  LGVGSVDDTPVELPKKLYIGPPSAKTIQLPDGRHLAYKEQGVTADRARFSLIAPHYFLSS 137
           LGVGSVDDTPVELPKKLYIGPPSAKTIQLPDGRHLAYKEQGVTADRARFSLIAPH FLSS
Sbjct: 145 LGVGSVDDTPVELPKKLYIGPPSAKTIQLPDGRHLAYKEQGVTADRARFSLIAPHSFLSS 204

Query: 138 RLAGIPGIKPSLLEKFGARLVIIN 161
           RLAGIPGIKPSLLE+FGARLV  +
Sbjct: 205 RLAGIPGIKPSLLEEFGARLVTYD 228



>Os12g0250850 
          Length = 223

 Score =  123 bits (308), Expect = 6e-29,   Method: Compositional matrix adjust.
 Identities = 59/76 (77%), Positives = 63/76 (82%)

Query: 1   CQIHWQAVDPAFGLPPVDPTAQSLAGAEDSELARRLRGPAVAMGKRLSFMNAYLAEDCNP 60
           CQI WQA+D A  L PVD  AQSLA  EDSELARRLRGPAVA+GKRLSFMN YLAED +P
Sbjct: 120 CQIRWQAIDLALRLSPVDLAAQSLASTEDSELARRLRGPAVAVGKRLSFMNEYLAEDRDP 179

Query: 61  VRCWVITAAVAFVTLI 76
            RCWV+TAAVAFVT I
Sbjct: 180 FRCWVVTAAVAFVTFI 195



>Os07g0289600 Hypothetical protein
          Length = 53

 Score = 89.0 bits (219), Expect = 1e-18,   Method: Compositional matrix adjust.
 Identities = 43/44 (97%), Positives = 43/44 (97%)

Query: 74  TLIVLGVGSVDDTPVELPKKLYIGPPSAKTIQLPDGRHLAYKEQ 117
           TLIVLGVGSVDDTPVELPKKLYIGPPSAKTIQL DGRHLAYKEQ
Sbjct: 10  TLIVLGVGSVDDTPVELPKKLYIGPPSAKTIQLSDGRHLAYKEQ 53



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.139    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,891,173
Number of extensions: 234737
Number of successful extensions: 585
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 587
Number of HSP's successfully gapped: 4
Length of query: 161
Length of database: 17,035,801
Length adjustment: 93
Effective length of query: 68
Effective length of database: 12,179,899
Effective search space: 828233132
Effective search space used: 828233132
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 152 (63.2 bits)