BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0533500 Os12g0533500|AK068646
         (109 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0533500  Conserved hypothetical protein                      125   6e-30

>Os12g0533500 Conserved hypothetical protein
          Length = 109

 Score =  125 bits (314), Expect = 6e-30,   Method: Compositional matrix adjust.
 Identities = 67/109 (61%), Positives = 67/109 (61%)

Query: 1   FFPSFHCKRSKSGXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGVXXXXXXXXXXXXXXGW 60
           FFPSFHCKRSKSG                            VGV              GW
Sbjct: 1   FFPSFHCKRSKSGSSRKTPSCPRPPPPPPPRSRPSAVSLSPVGVRRRRRRPPLLLLLLGW 60

Query: 61  PRAPEGTRRRRGSSLLPYTLVVVQAHLKNIYMLMIATPFQIIPWNSWVF 109
           PRAPEGTRRRRGSSLLPYTLVVVQAHLKNIYMLMIATPFQIIPWNSWVF
Sbjct: 61  PRAPEGTRRRRGSSLLPYTLVVVQAHLKNIYMLMIATPFQIIPWNSWVF 109



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.141    0.486 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,344,206
Number of extensions: 53613
Number of successful extensions: 156
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 155
Number of HSP's successfully gapped: 1
Length of query: 109
Length of database: 17,035,801
Length adjustment: 77
Effective length of query: 32
Effective length of database: 13,015,323
Effective search space: 416490336
Effective search space used: 416490336
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 149 (62.0 bits)