BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0525300 Os12g0525300|Os12g0525300
         (148 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0525300                                                      270   3e-73

>Os12g0525300 
          Length = 148

 Score =  270 bits (689), Expect = 3e-73,   Method: Compositional matrix adjust.
 Identities = 136/148 (91%), Positives = 136/148 (91%)

Query: 1   MEIGSKRAELGIRDTVGWLNGRNAILMGTGKRMVPNTGPXXXXXXXXXXXXINTLPTKLS 60
           MEIGSKRAELGIRDTVGWLNGRNAILMGTGKRMVPNTGP            INTLPTKLS
Sbjct: 1   MEIGSKRAELGIRDTVGWLNGRNAILMGTGKRMVPNTGPEEERRKKVVKEEINTLPTKLS 60

Query: 61  YVPTEPLIYNGKKEGYLGSKVTVRGNKKYGKMNERQKYRTYALSQNIYLLSIRQEGQGKD 120
           YVPTEPLIYNGKKEGYLGSKVTVRGNKKYGKMNERQKYRTYALSQNIYLLSIRQEGQGKD
Sbjct: 61  YVPTEPLIYNGKKEGYLGSKVTVRGNKKYGKMNERQKYRTYALSQNIYLLSIRQEGQGKD 120

Query: 121 NGYGTQVLALLENEALSVQTYEITYPGF 148
           NGYGTQVLALLENEALSVQTYEITYPGF
Sbjct: 121 NGYGTQVLALLENEALSVQTYEITYPGF 148



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,464,349
Number of extensions: 156575
Number of successful extensions: 264
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 264
Number of HSP's successfully gapped: 1
Length of query: 148
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 56
Effective length of database: 12,232,113
Effective search space: 684998328
Effective search space used: 684998328
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 151 (62.8 bits)