BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0479100 Os12g0479100|Os12g0479100
         (214 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0479100  Conserved hypothetical protein                      239   1e-63
Os04g0396500                                                       89   2e-18

>Os12g0479100 Conserved hypothetical protein
          Length = 214

 Score =  239 bits (609), Expect = 1e-63,   Method: Compositional matrix adjust.
 Identities = 135/214 (63%), Positives = 135/214 (63%)

Query: 1   MAQDPSHPHRQSKDTAAAXXXXXXXXXXXXXXXXLITAPPPRDDVAHQEPXXXXXXXXXX 60
           MAQDPSHPHRQSKDTAAA                LITAPPPRDDVAHQEP          
Sbjct: 1   MAQDPSHPHRQSKDTAAAPPPPPPQEQPQQQQPELITAPPPRDDVAHQEPSTSSSSGGGG 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXTDAHHEAAAGPSTSAADPQLPELRLSVLHXX 120
                                        TDAHHEAAAGPSTSAADPQLPELRLSVLH  
Sbjct: 61  TDAAGLSLQLGLGPSAPGSPSSSRRKRPRTDAHHEAAAGPSTSAADPQLPELRLSVLHPG 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXHEAGTWFVLRAAQNQRREPPLPQIPRSFLRVRDGRMTV 180
                                 HEAGTWFVLRAAQNQRREPPLPQIPRSFLRVRDGRMTV
Sbjct: 121 PGPSSSASAVGAVVAAAPPPPAHEAGTWFVLRAAQNQRREPPLPQIPRSFLRVRDGRMTV 180

Query: 181 RVVMRYLVNKLSLEDDSQVKGVRPSVTSWVTPHD 214
           RVVMRYLVNKLSLEDDSQVKGVRPSVTSWVTPHD
Sbjct: 181 RVVMRYLVNKLSLEDDSQVKGVRPSVTSWVTPHD 214



>Os04g0396500 
          Length = 372

 Score = 89.0 bits (219), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 40/58 (68%), Positives = 51/58 (87%)

Query: 145 AGTWFVLRAAQNQRREPPLPQIPRSFLRVRDGRMTVRVVMRYLVNKLSLEDDSQVKGV 202
           AG WFVL+AA  Q REP LPQIPRS+LR++DGR+TVR++ +YLVNKL LED+S+V+GV
Sbjct: 285 AGVWFVLQAAPLQGREPFLPQIPRSYLRIKDGRVTVRLLTKYLVNKLGLEDESEVRGV 342



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.129    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,375,817
Number of extensions: 136268
Number of successful extensions: 413
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 411
Number of HSP's successfully gapped: 3
Length of query: 214
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 117
Effective length of database: 11,971,043
Effective search space: 1400612031
Effective search space used: 1400612031
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 154 (63.9 bits)