BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0460000 Os12g0460000|AK068042
         (231 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0460000  Hypothetical protein                                390   e-109
Os12g0460400                                                       77   7e-15

>Os12g0460000 Hypothetical protein
          Length = 231

 Score =  390 bits (1003), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 195/231 (84%), Positives = 195/231 (84%)

Query: 1   MEGSSASQAKALFKATLLGLAILSMVTWVPHMYSCIKMFLVSVPSAASTLATPRCLFIFS 60
           MEGSSASQAKALFKATLLGLAILSMVTWVPHMYSCIKMFLVSVPSAASTLATPRCLFIFS
Sbjct: 1   MEGSSASQAKALFKATLLGLAILSMVTWVPHMYSCIKMFLVSVPSAASTLATPRCLFIFS 60

Query: 61  NIIVIFLASELKLSEGESFGESPIPTNHGSDDSIRYRVEAFTLTTKSNDVIVNHVVEEQV 120
           NIIVIFLASELKLSEGESFGESPIPTNHGSDDSIRYRVEAFTLTTKSNDVIVNHVVEEQV
Sbjct: 61  NIIVIFLASELKLSEGESFGESPIPTNHGSDDSIRYRVEAFTLTTKSNDVIVNHVVEEQV 120

Query: 121 STVIVHDDSLQQLDQCEQVDASSTMSMDKESRRDXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           STVIVHDDSLQQLDQCEQVDASSTMSMDKESRRD                          
Sbjct: 121 STVIVHDDSLQQLDQCEQVDASSTMSMDKESRRDNNNNNLAIGANVGNNGESEEVEEQGG 180

Query: 181 XISLGKVXXXXXXXXXDVGLPTDELNRRVEDFIARFNMERQLEARMLVCCY 231
            ISLGKV         DVGLPTDELNRRVEDFIARFNMERQLEARMLVCCY
Sbjct: 181 AISLGKVIEEEMIEEEDVGLPTDELNRRVEDFIARFNMERQLEARMLVCCY 231



>Os12g0460400 
          Length = 344

 Score = 77.4 bits (189), Expect = 7e-15,   Method: Compositional matrix adjust.
 Identities = 34/66 (51%), Positives = 46/66 (69%), Gaps = 1/66 (1%)

Query: 10 KALFKATLLGLAILSMVTWVPHMYSCIK-MFLVSVPSAASTLATPRCLFIFSNIIVIFLA 68
          + + K +LL L IL M TWVPHMYS ++ +F+V +PS  S +  P+CLF+FSNIIV+FL 
Sbjct: 4  QEIVKISLLALGILFMATWVPHMYSFMRALFMVYLPSFTSAVVAPKCLFVFSNIIVVFLV 63

Query: 69 SELKLS 74
           E K  
Sbjct: 64 GESKFG 69



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.132    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,583,695
Number of extensions: 176655
Number of successful extensions: 461
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 459
Number of HSP's successfully gapped: 2
Length of query: 231
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 133
Effective length of database: 11,918,829
Effective search space: 1585204257
Effective search space used: 1585204257
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 154 (63.9 bits)