BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0413400 Os12g0413400|AK062663
         (196 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0413400  Conserved hypothetical protein                      161   3e-40

>Os12g0413400 Conserved hypothetical protein
          Length = 196

 Score =  161 bits (408), Expect = 3e-40,   Method: Compositional matrix adjust.
 Identities = 97/178 (54%), Positives = 97/178 (54%)

Query: 1   RTAEKQLEPALWRACLRLTVXXXXXXXXXXXXXXXXXXXXXXXXETLTPAGSGDEVRRTM 60
           RTAEKQLEPALWRACLRLTV                        ETLTPAGSGDEVRRTM
Sbjct: 1   RTAEKQLEPALWRACLRLTVTSRPLTSRHRPRRHPRALLRAAASETLTPAGSGDEVRRTM 60

Query: 61  TTSGSVSSSPAERGGVQIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYLATR 120
           TTSGSVSSSPAERGGVQI                                     YLATR
Sbjct: 61  TTSGSVSSSPAERGGVQIRAAAAARPGGEESRRRRSEEGRVGSARPRGRRHRRSRYLATR 120

Query: 121 AAVXXXXXXXXXXDKEXXXXXXXXXXPPWSRVRESLIVVHMLRSALPTSVSPGSSLPC 178
           AAV          DKE          PPWSRVRESLIVVHMLRSALPTSVSPGSSLPC
Sbjct: 121 AAVPPSRSSPSGPDKERRGRAVRRRAPPWSRVRESLIVVHMLRSALPTSVSPGSSLPC 178



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.126    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,346,242
Number of extensions: 69184
Number of successful extensions: 157
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 155
Number of HSP's successfully gapped: 2
Length of query: 196
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 100
Effective length of database: 12,023,257
Effective search space: 1202325700
Effective search space used: 1202325700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 153 (63.5 bits)