BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0275100 Os12g0275100|Os12g0275100
         (115 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0275100                                                      241   1e-64
Os12g0275201  Hypothetical protein                                151   1e-37
Os12g0275666                                                      148   9e-37

>Os12g0275100 
          Length = 115

 Score =  241 bits (614), Expect = 1e-64,   Method: Compositional matrix adjust.
 Identities = 115/115 (100%), Positives = 115/115 (100%)

Query: 1   MANIPHIAVPAQGKICEGSTGGCICAMYTGEVKGPVHHDIDTSANGMLVCTTIDKGGLPM 60
           MANIPHIAVPAQGKICEGSTGGCICAMYTGEVKGPVHHDIDTSANGMLVCTTIDKGGLPM
Sbjct: 1   MANIPHIAVPAQGKICEGSTGGCICAMYTGEVKGPVHHDIDTSANGMLVCTTIDKGGLPM 60

Query: 61  YHLVGVNTDFNWEIKHDRRWIDMMKDSISAFGFCHSMQLHSGLQTNLIDAVDVGR 115
           YHLVGVNTDFNWEIKHDRRWIDMMKDSISAFGFCHSMQLHSGLQTNLIDAVDVGR
Sbjct: 61  YHLVGVNTDFNWEIKHDRRWIDMMKDSISAFGFCHSMQLHSGLQTNLIDAVDVGR 115



>Os12g0275201 Hypothetical protein
          Length = 272

 Score =  151 bits (381), Expect = 1e-37,   Method: Compositional matrix adjust.
 Identities = 69/74 (93%), Positives = 72/74 (97%)

Query: 31  EVKGPVHHDIDTSANGMLVCTTIDKGGLPMYHLVGVNTDFNWEIKHDRRWIDMMKDSISA 90
           EVKGPVHHDIDTSANGMLVCTTIDKGGL +YHLVGVNTDF+WEIKH+RRWIDMMKDSISA
Sbjct: 192 EVKGPVHHDIDTSANGMLVCTTIDKGGLVVYHLVGVNTDFSWEIKHERRWIDMMKDSISA 251

Query: 91  FGFCHSMQLHSGLQ 104
           FGFCHSMQL SGLQ
Sbjct: 252 FGFCHSMQLRSGLQ 265



>Os12g0275666 
          Length = 487

 Score =  148 bits (373), Expect = 9e-37,   Method: Compositional matrix adjust.
 Identities = 68/74 (91%), Positives = 71/74 (95%)

Query: 31  EVKGPVHHDIDTSANGMLVCTTIDKGGLPMYHLVGVNTDFNWEIKHDRRWIDMMKDSISA 90
           EVKGPVHHDIDTSANGMLVCTTIDKGGL +YHLV VNTDF+WEIKH+RRWIDMMKDSISA
Sbjct: 315 EVKGPVHHDIDTSANGMLVCTTIDKGGLIVYHLVRVNTDFSWEIKHERRWIDMMKDSISA 374

Query: 91  FGFCHSMQLHSGLQ 104
           FGFCHSMQL SGLQ
Sbjct: 375 FGFCHSMQLRSGLQ 388




 Score = 65.1 bits (157), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 28/31 (90%), Positives = 28/31 (90%)

Query: 1   MANIPHIAVPAQGKICEGSTGGCICAMYTGE 31
           MANIPHIAVP QGKICEG  GGCICAMYTGE
Sbjct: 456 MANIPHIAVPVQGKICEGRAGGCICAMYTGE 486



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.139    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,415,931
Number of extensions: 176407
Number of successful extensions: 282
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 282
Number of HSP's successfully gapped: 4
Length of query: 115
Length of database: 17,035,801
Length adjustment: 82
Effective length of query: 33
Effective length of database: 12,754,253
Effective search space: 420890349
Effective search space used: 420890349
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 149 (62.0 bits)