BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0227400 Os12g0227400|J100082K03
         (62 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0227400  Similar to Allyl alcohol dehydrogenase               97   3e-21
>Os12g0227400 Similar to Allyl alcohol dehydrogenase
          Length = 62

 Score = 97.1 bits (240), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 50/62 (80%), Positives = 50/62 (80%)

Query: 1  NQCPLIAPVXXXXXXXXXXXXQVNLLKTKFGFDDAFNYKKELDLEAALKRSALLHILSNI 60
          NQCPLIAPV            QVNLLKTKFGFDDAFNYKKELDLEAALKRSALLHILSNI
Sbjct: 1  NQCPLIAPVLLHLLRIHRLHLQVNLLKTKFGFDDAFNYKKELDLEAALKRSALLHILSNI 60

Query: 61 IK 62
          IK
Sbjct: 61 IK 62
  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.139    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,327,867
Number of extensions: 27762
Number of successful extensions: 34
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 1
Length of query: 62
Length of database: 17,035,801
Length adjustment: 34
Effective length of query: 28
Effective length of database: 15,260,525
Effective search space: 427294700
Effective search space used: 427294700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)