BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0131100 Os12g0131100|AK105856
         (320 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0131100  Similar to Glucosamine--fructose-6-phosphate a...   574   e-164
AK107318                                                          340   1e-93

>Os12g0131100 Similar to Glucosamine--fructose-6-phosphate aminotransferase
           [isomerizing] (EC 2.6.1.16) (Hexosephosphate
           aminotransferase) (D-fructose-6- phosphate
           amidotransferase) (GFAT)
          Length = 320

 Score =  574 bits (1480), Expect = e-164,   Method: Compositional matrix adjust.
 Identities = 295/320 (92%), Positives = 295/320 (92%)

Query: 1   RRSRRLVFIGCGTSYNAALAARPFVEELTGIPVTMEVASDLLDRQGPIYREDTAVFVSQS 60
           RRSRRLVFIGCGTSYNAALAARPFVEELTGIPVTMEVASDLLDRQGPIYREDTAVFVSQS
Sbjct: 1   RRSRRLVFIGCGTSYNAALAARPFVEELTGIPVTMEVASDLLDRQGPIYREDTAVFVSQS 60

Query: 61  GEXXXXXXXXXXXXENGALCVGITNTVGSTLSRRTHCGVHINAGCEIGVASTKAYTSQIV 120
           GE            ENGALCVGITNTVGSTLSRRTHCGVHINAGCEIGVASTKAYTSQIV
Sbjct: 61  GETADTLLALDYALENGALCVGITNTVGSTLSRRTHCGVHINAGCEIGVASTKAYTSQIV 120

Query: 121 VMVMVALAVGSDQISTQVRRQAIISGLSNLPSNVSEVLKLDTEMKXXXXXXXXXXXXXVF 180
           VMVMVALAVGSDQISTQVRRQAIISGLSNLPSNVSEVLKLDTEMK             VF
Sbjct: 121 VMVMVALAVGSDQISTQVRRQAIISGLSNLPSNVSEVLKLDTEMKELSSSLIDSESLLVF 180

Query: 181 GRGYNYATALEGALKVKEVALMHSEGMLAGEMKHGPLALVDENLPIIVIATRDACFSKQQ 240
           GRGYNYATALEGALKVKEVALMHSEGMLAGEMKHGPLALVDENLPIIVIATRDACFSKQQ
Sbjct: 181 GRGYNYATALEGALKVKEVALMHSEGMLAGEMKHGPLALVDENLPIIVIATRDACFSKQQ 240

Query: 241 SVIQQLLSRKGRLIVMCSKGDASAVCPSGSCRVIEVPEVADCLQPVINIIPLQLLAYHLT 300
           SVIQQLLSRKGRLIVMCSKGDASAVCPSGSCRVIEVPEVADCLQPVINIIPLQLLAYHLT
Sbjct: 241 SVIQQLLSRKGRLIVMCSKGDASAVCPSGSCRVIEVPEVADCLQPVINIIPLQLLAYHLT 300

Query: 301 VLRGFDVDQPRNLAKSVTTQ 320
           VLRGFDVDQPRNLAKSVTTQ
Sbjct: 301 VLRGFDVDQPRNLAKSVTTQ 320



>AK107318 
          Length = 697

 Score =  340 bits (871), Expect = 1e-93,   Method: Compositional matrix adjust.
 Identities = 168/320 (52%), Positives = 218/320 (68%), Gaps = 2/320 (0%)

Query: 1   RRSRRLVFIGCGTSYNAALAARPFVEELTGIPVTMEVASDLLDRQGPIYREDTAVFVSQS 60
           RR RRL+FI CGTSY++ +A R   E+LT IP+++E+ASD LDRQ P++R+DT VFVSQS
Sbjct: 380 RRCRRLIFIACGTSYHSCMAVRGAFEDLTEIPISVELASDFLDRQAPVFRDDTCVFVSQS 439

Query: 61  GEXXXXXXXXXXXXENGALCVGITNTVGSTLSRRTHCGVHINAGCEIGVASTKAYTSQIV 120
           GE            E GAL VGI N VGS++S  THCGVHINAG EIGVASTKAYTSQ V
Sbjct: 440 GETADSLMALRYCLERGALTVGIVNVVGSSISLMTHCGVHINAGPEIGVASTKAYTSQFV 499

Query: 121 VMVMVALAVGSDQISTQVRRQAIISGLSNLPSNVSEVLKLDTEMKXXXXXXXXXXXXXVF 180
            M+M AL +  D+ S   +R  I+ GLS +     E+LKLD  +K             + 
Sbjct: 500 CMIMFALTLSEDRASKTQKRLEIMEGLSKISEQFREILKLDQSIKEMCLKFKDQKSLLLL 559

Query: 181 GRGYNYATALEGALKVKEVALMHSEGMLAGEMKHGPLALVDENLPIIVIATRDACFSKQQ 240
           GRG  +ATALEGALK+KE++ +H E +++GE+KHG LALVDENLPI++I TRD+ F+K  
Sbjct: 560 GRGSQHATALEGALKIKEISYLHCEAVMSGELKHGVLALVDENLPIVMILTRDSIFAKSL 619

Query: 241 SVIQQLLSRKGRLIVMCSKGDASAVCPSGSCRVIEVPEVADCLQPVINIIPLQLLAYHLT 300
           +  QQ+++RKGR IV+C++ D     P      IEVP   + LQ ++N+IPLQL+AY L 
Sbjct: 620 NAYQQVIARKGRPIVICNQDDEE--FPGEKTDKIEVPHTHELLQGLLNVIPLQLMAYWLA 677

Query: 301 VLRGFDVDQPRNLAKSVTTQ 320
           V  G +VD PRNLAKSVT +
Sbjct: 678 VAEGLNVDFPRNLAKSVTVE 697



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.134    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,651,207
Number of extensions: 294924
Number of successful extensions: 609
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 608
Number of HSP's successfully gapped: 2
Length of query: 320
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 219
Effective length of database: 11,762,187
Effective search space: 2575918953
Effective search space used: 2575918953
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)