BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0103300 Os12g0103300|J065206G13
         (102 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0103300  Sec61beta family protein                            129   5e-31
Os11g0103900  Sec61beta family protein                            108   1e-24

>Os12g0103300 Sec61beta family protein
          Length = 102

 Score =  129 bits (324), Expect = 5e-31,   Method: Compositional matrix adjust.
 Identities = 69/102 (67%), Positives = 69/102 (67%)

Query: 1   VDYSSSRLPVFHPNRHXXXXXXXXXXXXXXXXXXXXXTAXXXXXXXXXXXXNMLRFYTDE 60
           VDYSSSRLPVFHPNRH                     TA            NMLRFYTDE
Sbjct: 1   VDYSSSRLPVFHPNRHGSLLLPVPVLRRRRRRGRPPSTAGGSGGFSGGGGSNMLRFYTDE 60

Query: 61  APGLRLSPTMVLVMSLCFIGFVTALHVFGKLYRSRTAASASA 102
           APGLRLSPTMVLVMSLCFIGFVTALHVFGKLYRSRTAASASA
Sbjct: 61  APGLRLSPTMVLVMSLCFIGFVTALHVFGKLYRSRTAASASA 102



>Os11g0103900 Sec61beta family protein
          Length = 107

 Score =  108 bits (269), Expect = 1e-24,   Method: Compositional matrix adjust.
 Identities = 51/51 (100%), Positives = 51/51 (100%)

Query: 52  NMLRFYTDEAPGLRLSPTMVLVMSLCFIGFVTALHVFGKLYRSRTAASASA 102
           NMLRFYTDEAPGLRLSPTMVLVMSLCFIGFVTALHVFGKLYRSRTAASASA
Sbjct: 57  NMLRFYTDEAPGLRLSPTMVLVMSLCFIGFVTALHVFGKLYRSRTAASASA 107



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.327    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,963,960
Number of extensions: 34527
Number of successful extensions: 85
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 84
Number of HSP's successfully gapped: 2
Length of query: 102
Length of database: 17,035,801
Length adjustment: 70
Effective length of query: 32
Effective length of database: 13,380,821
Effective search space: 428186272
Effective search space used: 428186272
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 149 (62.0 bits)