BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0659200 Os11g0659200|J075043D24
         (150 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0659200  Protein of unknown function DUF581 family protein   231   2e-61
Os02g0592800  Protein of unknown function DUF581 family protein    80   4e-16

>Os11g0659200 Protein of unknown function DUF581 family protein
          Length = 150

 Score =  231 bits (589), Expect = 2e-61,   Method: Compositional matrix adjust.
 Identities = 120/150 (80%), Positives = 120/150 (80%)

Query: 1   MAGLCVLLEKQHXXXXXXXXXXXXXXXQVISKTAVLSTAGGGGSKIHGCYXXXXXXXRAP 60
           MAGLCVLLEKQH               QVISKTAVLSTAGGGGSKIHGCY       RAP
Sbjct: 1   MAGLCVLLEKQHAAAMTTATAAAARTAQVISKTAVLSTAGGGGSKIHGCYSSSSSSSRAP 60

Query: 61  VVVATTASFLHRCFLCRRELAGGDDIYIYRGDRAFCSDDCRFRHILTEEEEEDGDMSCRA 120
           VVVATTASFLHRCFLCRRELAGGDDIYIYRGDRAFCSDDCRFRHILTEEEEEDGDMSCRA
Sbjct: 61  VVVATTASFLHRCFLCRRELAGGDDIYIYRGDRAFCSDDCRFRHILTEEEEEDGDMSCRA 120

Query: 121 KDAAVAAPTXXXXXXXXAVAGCGGSGFFAY 150
           KDAAVAAPT        AVAGCGGSGFFAY
Sbjct: 121 KDAAVAAPTRRRSRNRRAVAGCGGSGFFAY 150



>Os02g0592800 Protein of unknown function DUF581 family protein
          Length = 126

 Score = 80.5 bits (197), Expect = 4e-16,   Method: Compositional matrix adjust.
 Identities = 47/110 (42%), Positives = 55/110 (50%), Gaps = 16/110 (14%)

Query: 1   MAGLCVLLEKQHXXXXXXXXXXXXXXXQVISKTAVLSTAGGGGSKIHGCYXXXXXXXRAP 60
           MAGL VLLE                  Q+ISK           + +H           A 
Sbjct: 1   MAGLSVLLETH-----KNDHHPNMRPPQIISK-----------ATLHSHPETMSSSSPAT 44

Query: 61  VVVATTASFLHRCFLCRRELAGGDDIYIYRGDRAFCSDDCRFRHILTEEE 110
              AT +SFL RCFLCRRELA G DIY+YRGDRAFCS DCR + I  +E+
Sbjct: 45  ATTATMSSFLQRCFLCRRELADGKDIYMYRGDRAFCSVDCRCKQIFMDED 94



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.139    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,130,892
Number of extensions: 210164
Number of successful extensions: 543
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 545
Number of HSP's successfully gapped: 2
Length of query: 150
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 58
Effective length of database: 12,232,113
Effective search space: 709462554
Effective search space used: 709462554
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 151 (62.8 bits)