BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0490800 Os11g0490800|AK106821
         (145 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0490800  Similar to Spermidine/putrescine import ATP-bi...   261   1e-70
AK111219                                                          120   5e-28

>Os11g0490800 Similar to Spermidine/putrescine import ATP-binding protein potA
           (EC 3.6.3.31)
          Length = 145

 Score =  261 bits (668), Expect = 1e-70,   Method: Compositional matrix adjust.
 Identities = 132/145 (91%), Positives = 132/145 (91%)

Query: 1   DRLHISCRYFLADTVLEEITFGWPRQNTDFLFREKLALKLQNAINSVGLNGISLEEDPQS 60
           DRLHISCRYFLADTVLEEITFGWPRQNTDFLFREKLALKLQNAINSVGLNGISLEEDPQS
Sbjct: 1   DRLHISCRYFLADTVLEEITFGWPRQNTDFLFREKLALKLQNAINSVGLNGISLEEDPQS 60

Query: 61  LSGGFKRRLALAIQLVQTPXXXXXXXXXXXXXWKARADVVNLLKDLKKDHTILAVSHDLR 120
           LSGGFKRRLALAIQLVQTP             WKARADVVNLLKDLKKDHTILAVSHDLR
Sbjct: 61  LSGGFKRRLALAIQLVQTPDLLLLDEPLAGLDWKARADVVNLLKDLKKDHTILAVSHDLR 120

Query: 121 ELYPLVDRSWRMEMGGVLKEEALSV 145
           ELYPLVDRSWRMEMGGVLKEEALSV
Sbjct: 121 ELYPLVDRSWRMEMGGVLKEEALSV 145



>AK111219 
          Length = 422

 Score =  120 bits (300), Expect = 5e-28,   Method: Compositional matrix adjust.
 Identities = 59/132 (44%), Positives = 85/132 (64%)

Query: 8   RYFLADTVLEEITFGWPRQNTDFLFREKLALKLQNAINSVGLNGISLEEDPQSLSGGFKR 67
           R+FL D V+ E+TF WPR+   +  R+ +A+++Q  I +VGL  I     P  LSGG +R
Sbjct: 277 RHFLGDDVMTEMTFTWPREVAYWGQRQAMAVRMQRVIEAVGLTDIPFNISPSDLSGGQQR 336

Query: 68  RLALAIQLVQTPXXXXXXXXXXXXXWKARADVVNLLKDLKKDHTILAVSHDLRELYPLVD 127
           RLALA+QLV+ P             W AR +VV  L++LK++ T+L VSHDL ++ PLVD
Sbjct: 337 RLALALQLVRGPSVLLLDEPLAGLDWHARREVVAPLRELKREATLLVVSHDLADIAPLVD 396

Query: 128 RSWRMEMGGVLK 139
            +WRM+MGG ++
Sbjct: 397 CAWRMQMGGRME 408



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.138    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,125,056
Number of extensions: 129174
Number of successful extensions: 294
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 294
Number of HSP's successfully gapped: 2
Length of query: 145
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 54
Effective length of database: 12,284,327
Effective search space: 663353658
Effective search space used: 663353658
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 151 (62.8 bits)