BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0127800 Os11g0127800|AK059969
         (230 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0127800  SMAD/FHA domain containing protein                  279   1e-75
Os12g0124000  SMAD/FHA domain containing protein                  260   7e-70

>Os11g0127800 SMAD/FHA domain containing protein
          Length = 230

 Score =  279 bits (713), Expect = 1e-75,   Method: Compositional matrix adjust.
 Identities = 140/173 (80%), Positives = 140/173 (80%)

Query: 37  YASHGFKLLQPQLLFINRLTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERWVLEP 96
           YASHGFKLLQPQLLFINRLT                                 ERWVLEP
Sbjct: 37  YASHGFKLLQPQLLFINRLTSRNSNGSSRRSISILSLRCSSSGTDSASSSATSERWVLEP 96

Query: 97  AGDGDWRHIGYRVARPGGFQIASEAAVTVGRVPEQADIVLSVATVSGTHARLEKKEGSLL 156
           AGDGDWRHIGYRVARPGGFQIASEAAVTVGRVPEQADIVLSVATVSGTHARLEKKEGSLL
Sbjct: 97  AGDGDWRHIGYRVARPGGFQIASEAAVTVGRVPEQADIVLSVATVSGTHARLEKKEGSLL 156

Query: 157 VTDLESTNGTYINERRLTPGFPTPIDPGSLLIFGDIHLAMFRVSKMIVDVSSD 209
           VTDLESTNGTYINERRLTPGFPTPIDPGSLLIFGDIHLAMFRVSKMIVDVSSD
Sbjct: 157 VTDLESTNGTYINERRLTPGFPTPIDPGSLLIFGDIHLAMFRVSKMIVDVSSD 209



>Os12g0124000 SMAD/FHA domain containing protein
          Length = 228

 Score =  260 bits (664), Expect = 7e-70,   Method: Compositional matrix adjust.
 Identities = 129/173 (74%), Positives = 136/173 (78%)

Query: 37  YASHGFKLLQPQLLFINRLTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERWVLEP 96
           YAS+G KL QP+L F+++LT                                 ERWVLEP
Sbjct: 34  YASNGAKLQQPRLQFVSQLTSRNSNGSGRRSISILSLRCSSSGTDSASSSATSERWVLEP 93

Query: 97  AGDGDWRHIGYRVARPGGFQIASEAAVTVGRVPEQADIVLSVATVSGTHARLEKKEGSLL 156
           AGDGDWRHIGYRVARPGGFQIASEAAVTVGRVPEQADIVLSVATVSGTHARLEKKEGSLL
Sbjct: 94  AGDGDWRHIGYRVARPGGFQIASEAAVTVGRVPEQADIVLSVATVSGTHARLEKKEGSLL 153

Query: 157 VTDLESTNGTYINERRLTPGFPTPIDPGSLLIFGDIHLAMFRVSKMIVDVSSD 209
           VTDLESTNGTYINERRL+PGFPTPIDPGSLLIFGDIHLAMFRVSKM+VDV SD
Sbjct: 154 VTDLESTNGTYINERRLSPGFPTPIDPGSLLIFGDIHLAMFRVSKMVVDVPSD 206



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,048,568
Number of extensions: 173998
Number of successful extensions: 403
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 401
Number of HSP's successfully gapped: 2
Length of query: 230
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 133
Effective length of database: 11,971,043
Effective search space: 1592148719
Effective search space used: 1592148719
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 154 (63.9 bits)