BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0577400 Os10g0577400|AK064295
         (350 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0577400  Conserved hypothetical protein                      456   e-128

>Os10g0577400 Conserved hypothetical protein
          Length = 350

 Score =  456 bits (1172), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 245/350 (70%), Positives = 245/350 (70%)

Query: 1   METPLSSRRIXXXXXXXXXXXXXXXXXXXXXRPALHDITNDSPIVGLASSGLRTPASTAA 60
           METPLSSRRI                     RPALHDITNDSPIVGLASSGLRTPASTAA
Sbjct: 1   METPLSSRRITRSMAKASAAAPDATSTAHPPRPALHDITNDSPIVGLASSGLRTPASTAA 60

Query: 61  KARPTRASRRTPGSGEALLRGQVKTLLQKVHHDXXXXXXXXXXXXXXXNILRIHAPIGLA 120
           KARPTRASRRTPGSGEALLRGQVKTLLQKVHHD               NILRIHAPIGLA
Sbjct: 61  KARPTRASRRTPGSGEALLRGQVKTLLQKVHHDASSSSSCAAAAPPSPNILRIHAPIGLA 120

Query: 121 RSPAHLLAPTPANTPHLTAAAPHAFTMTVPCVLEEELLSKLQVIADALPPPPAQAEENHL 180
           RSPAHLLAPTPANTPHLTAAAPHAFTMTVPCVLEEELLSKLQVIADALPPPPAQAEENHL
Sbjct: 121 RSPAHLLAPTPANTPHLTAAAPHAFTMTVPCVLEEELLSKLQVIADALPPPPAQAEENHL 180

Query: 181 GECNRALVFDDSPGKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIQVNASSEK 240
           GECNRALVFDDSPGK                                    IQVNASSEK
Sbjct: 181 GECNRALVFDDSPGKSDLSNAASVLSSSSLSFQDSSSTDRSPDDDSSSAWSIQVNASSEK 240

Query: 241 GXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGMSKMSVFDDEEEEDKKAGLPAFQGK 300
           G                                 GMSKMSVFDDEEEEDKKAGLPAFQGK
Sbjct: 241 GDEDTFTDQDPEEEEEEWLTEDDDDDECFDDLCEGMSKMSVFDDEEEEDKKAGLPAFQGK 300

Query: 301 HTRFIYDSDGEMEREDVAHVPVENCTMVLRGLPVPEGKHLRFHEVEEDEE 350
           HTRFIYDSDGEMEREDVAHVPVENCTMVLRGLPVPEGKHLRFHEVEEDEE
Sbjct: 301 HTRFIYDSDGEMEREDVAHVPVENCTMVLRGLPVPEGKHLRFHEVEEDEE 350



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.131    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,609,745
Number of extensions: 383885
Number of successful extensions: 1347
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 1342
Number of HSP's successfully gapped: 1
Length of query: 350
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 248
Effective length of database: 11,709,973
Effective search space: 2904073304
Effective search space used: 2904073304
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 156 (64.7 bits)