BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0534400 Os10g0534400|AK105216
         (268 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0534400  Similar to E4 protein (Fragment)                    303   8e-83

>Os10g0534400 Similar to E4 protein (Fragment)
          Length = 268

 Score =  303 bits (776), Expect = 8e-83,   Method: Compositional matrix adjust.
 Identities = 166/268 (61%), Positives = 166/268 (61%)

Query: 1   MEASPSRSDSFSHGWLXXXXXXXXXXXSFERLXXXXXXXXXXXXXLGHSFNGSVVSFIDM 60
           MEASPSRSDSFSHGWL           SFERL             LGHSFNGSVVSFIDM
Sbjct: 1   MEASPSRSDSFSHGWLRRKARPAAAAASFERLVGGEGDAVDVDGDLGHSFNGSVVSFIDM 60

Query: 61  DPAELFSMRWTSLTAAAXXXXXXXXXXXXXGMPCVAGAQCSSPLLVGAGRALSDGHHHHL 120
           DPAELFSMRWTSLTAAA             GMPCVAGAQCSSPLLVGAGRALSDGHHHHL
Sbjct: 61  DPAELFSMRWTSLTAAAEEEEDHDDDDFDFGMPCVAGAQCSSPLLVGAGRALSDGHHHHL 120

Query: 121 LLPCEPGVVVARDRTXXXXXXXXXXXXXXXXXXXXXTPASVITTXXXXXXXXXXXXXXXP 180
           LLPCEPGVVVARDRT                     TPASVITT               P
Sbjct: 121 LLPCEPGVVVARDRTASYADAPSPSPSSPLFHSALSTPASVITTASSRRAGAGSGKARAP 180

Query: 181 LLATRRILLRYLRFLVPLCRKVRSLPLRVLSPXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           LLATRRILLRYLRFLVPLCRKVRSLPLRVLSP                            
Sbjct: 181 LLATRRILLRYLRFLVPLCRKVRSLPLRVLSPRSAKGSLAAASSSASPARRSTSSSYASA 240

Query: 241 XEYWCHGNADTAVRDAILYCKKSIGQDM 268
            EYWCHGNADTAVRDAILYCKKSIGQDM
Sbjct: 241 AEYWCHGNADTAVRDAILYCKKSIGQDM 268



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.137    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,559,419
Number of extensions: 133984
Number of successful extensions: 452
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 450
Number of HSP's successfully gapped: 1
Length of query: 268
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 169
Effective length of database: 11,866,615
Effective search space: 2005457935
Effective search space used: 2005457935
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 155 (64.3 bits)