BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0514400 Os10g0514400|AK060321
         (142 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0514400  Similar to Phosphoenolpyruvate carboxykinase [...   212   9e-56

>Os10g0514400 Similar to Phosphoenolpyruvate carboxykinase [ATP] 1 (EC 4.1.1.49)
          Length = 142

 Score =  212 bits (539), Expect = 9e-56,   Method: Compositional matrix adjust.
 Identities = 111/142 (78%), Positives = 111/142 (78%)

Query: 1   RPCSPPAWPPCGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRMPVVLGGGAAPLIPR 60
           RPCSPPAWPPCGV                               RMPVVLGGGAAPLIPR
Sbjct: 1   RPCSPPAWPPCGVPSSPLTPVPLPYSSSSSPAPAFAPTTPSLTARMPVVLGGGAAPLIPR 60

Query: 61  TPAMTNEQRVVECGGGVNEVRRCAGRTHAVSAGRNAAARPSRACRDRGIPRRGGTASLTC 120
           TPAMTNEQRVVECGGGVNEVRRCAGRTHAVSAGRNAAARPSRACRDRGIPRRGGTASLTC
Sbjct: 61  TPAMTNEQRVVECGGGVNEVRRCAGRTHAVSAGRNAAARPSRACRDRGIPRRGGTASLTC 120

Query: 121 EWYALAEMIRTLIRFSVSQFKN 142
           EWYALAEMIRTLIRFSVSQFKN
Sbjct: 121 EWYALAEMIRTLIRFSVSQFKN 142



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.136    0.450 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,789,633
Number of extensions: 187964
Number of successful extensions: 810
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 808
Number of HSP's successfully gapped: 1
Length of query: 142
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 51
Effective length of database: 12,284,327
Effective search space: 626500677
Effective search space used: 626500677
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 151 (62.8 bits)