BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0508700 Os10g0508700|AK060639
         (214 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0508700  Pectinesterase inhibitor domain containing pro...   296   7e-81
Os04g0106000  Pectinesterase inhibitor domain containing pro...    68   5e-12

>Os10g0508700 Pectinesterase inhibitor domain containing protein
          Length = 214

 Score =  296 bits (758), Expect = 7e-81,   Method: Compositional matrix adjust.
 Identities = 157/214 (73%), Positives = 157/214 (73%)

Query: 1   MARXXXXXXXXVACFLVAGATAARPAPSTSGAAAISSFVRSWCAGTKYPALCDATLAPYS 60
           MAR        VACFLVAGATAARPAPSTSGAAAISSFVRSWCAGTKYPALCDATLAPYS
Sbjct: 1   MARSLSLLLLLVACFLVAGATAARPAPSTSGAAAISSFVRSWCAGTKYPALCDATLAPYS 60

Query: 61  AAVGASPAKLAWAALTVTLSAARNXXXXXXXXXXXXXXXHLPPVAAEAAGDCASELGDGV 120
           AAVGASPAKLAWAALTVTLSAARN               HLPPVAAEAAGDCASELGDGV
Sbjct: 61  AAVGASPAKLAWAALTVTLSAARNATSAVRSIAARSSSSHLPPVAAEAAGDCASELGDGV 120

Query: 121 DALRRCVDAMARXXXXXXXXXXXXXXXXXXXFEVDNVRTWASAALTDDNMCMEGFKXXXX 180
           DALRRCVDAMAR                   FEVDNVRTWASAALTDDNMCMEGFK    
Sbjct: 121 DALRRCVDAMARVAVGEESSSTAAAARRKVRFEVDNVRTWASAALTDDNMCMEGFKGEAA 180

Query: 181 XXXXXXXXXXXHIMGLLHLTANALGILNAMAKQI 214
                      HIMGLLHLTANALGILNAMAKQI
Sbjct: 181 GGGGAREAVRGHIMGLLHLTANALGILNAMAKQI 214



>Os04g0106000 Pectinesterase inhibitor domain containing protein
          Length = 177

 Score = 68.2 bits (165), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 54/179 (30%), Positives = 76/179 (42%), Gaps = 11/179 (6%)

Query: 33  AAISSFVRSWCAGTKYPALCDATLAPYSAAVGASPAKLAWAALTVTLSAARNXXXXXXXX 92
           AA + FVR  C  T+YP +C+ +L P + AVG SP  LA AALTV    AR+        
Sbjct: 3   AAAADFVRRSCRATRYPQVCERSLMPQAPAVGRSPRLLAQAALTVGADRARSCSGYLGGG 62

Query: 93  XXXXXXXHLPPVAAEAA-GDCASELGDGVDALRRCVDAMARXXXXXXXXXXXXXXXXXXX 151
                           A GDCA  L D  + LR+    M+R                   
Sbjct: 63  GSSSSSKRSGGGGRGGAVGDCADTLRDAEERLRQSAAEMSR---------MGRSGSPRFA 113

Query: 152 FEVDNVRTWASAALTDDNMCMEGFKXXXXXXXXXXXXXXXHIMGLLHLTANALGILNAM 210
           + + NV+TWASAALTD + C++                   +  +   T+NAL ++N +
Sbjct: 114 WRLSNVQTWASAALTDASTCLDSL-ATYAAPGIDVDALRKRVGAVSQATSNALALVNNL 171



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.128    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,158,777
Number of extensions: 150426
Number of successful extensions: 746
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 743
Number of HSP's successfully gapped: 4
Length of query: 214
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 117
Effective length of database: 11,971,043
Effective search space: 1400612031
Effective search space used: 1400612031
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 154 (63.9 bits)