BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0474600 Os10g0474600|Os10g0474600
         (288 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0474600  Conserved hypothetical protein                      328   3e-90

>Os10g0474600 Conserved hypothetical protein
          Length = 288

 Score =  328 bits (841), Expect = 3e-90,   Method: Compositional matrix adjust.
 Identities = 184/288 (63%), Positives = 184/288 (63%)

Query: 1   MKGDRMRKKQTKNYPSRIRSTGGQTTLQSFLFKSSVVDXXXXXXXXXXXXXXXXXXXXXQ 60
           MKGDRMRKKQTKNYPSRIRSTGGQTTLQSFLFKSSVVD                     Q
Sbjct: 1   MKGDRMRKKQTKNYPSRIRSTGGQTTLQSFLFKSSVVDEEVKPISPPPEKEEEEAISPPQ 60

Query: 61  PPKREIIRVTTKAIVKEKXXXXXXXXXXXXXXXXXXXXXXXXXXXLFRRFNSSAPVPRAE 120
           PPKREIIRVTTKAIVKEK                           LFRRFNSSAPVPRAE
Sbjct: 61  PPKREIIRVTTKAIVKEKASAFSSVGSSSSSGKSGAGGGALNAAALFRRFNSSAPVPRAE 120

Query: 121 AAGDGDGGVRLEIEDIAAGDRRRETRKRKSPLGARLASRIPSPPFRLSPLIRDAMSISLA 180
           AAGDGDGGVRLEIEDIAAGDRRRETRKRKSPLGARLASRIPSPPFRLSPLIRDAMSISLA
Sbjct: 121 AAGDGDGGVRLEIEDIAAGDRRRETRKRKSPLGARLASRIPSPPFRLSPLIRDAMSISLA 180

Query: 181 XXXXXXXXXXXXXXXKRVVVLGDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGRGEEGG 240
                          KRVVVLGDD                             GRGEEGG
Sbjct: 181 GGGGGGDDHHSGGSGKRVVVLGDDPKPRPAARTMTTTATTRRQRRGRRHAGPTGRGEEGG 240

Query: 241 DQAAYNHYASGGGWWHXXXXXXXXXXXXWTDDMWEGMGSITLGGLEWH 288
           DQAAYNHYASGGGWWH            WTDDMWEGMGSITLGGLEWH
Sbjct: 241 DQAAYNHYASGGGWWHGEMEGVDGEEVGWTDDMWEGMGSITLGGLEWH 288



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,271,205
Number of extensions: 253271
Number of successful extensions: 1143
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 1140
Number of HSP's successfully gapped: 1
Length of query: 288
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 188
Effective length of database: 11,814,401
Effective search space: 2221107388
Effective search space used: 2221107388
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 155 (64.3 bits)