BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0457400 Os10g0457400|AK063553
         (351 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0457400  Conserved hypothetical protein                      538   e-153

>Os10g0457400 Conserved hypothetical protein
          Length = 351

 Score =  538 bits (1386), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 281/351 (80%), Positives = 281/351 (80%)

Query: 1   MEFAVPVPQQVSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSSIPKGAGAAARRKTLCD 60
           MEFAVPVPQQVSS                             SSIPKGAGAAARRKTLCD
Sbjct: 1   MEFAVPVPQQVSSLALPVVVAEAEAAAAAAAAPGRRGAAGGVSSIPKGAGAAARRKTLCD 60

Query: 61  ITNLRPRPAAAVEQDGATCAADAGGVAQAQLVKENSELVRLLEERDKIIELSGTELQKLR 120
           ITNLRPRPAAAVEQDGATCAADAGGVAQAQLVKENSELVRLLEERDKIIELSGTELQKLR
Sbjct: 61  ITNLRPRPAAAVEQDGATCAADAGGVAQAQLVKENSELVRLLEERDKIIELSGTELQKLR 120

Query: 121 LANWQLAQANSQMLAELNLGRDRLKKLQHQLACSRAVIATKTSELEEAKKAMKRNRNLPL 180
           LANWQLAQANSQMLAELNLGRDRLKKLQHQLACSRAVIATKTSELEEAKKAMKRNRNLPL
Sbjct: 121 LANWQLAQANSQMLAELNLGRDRLKKLQHQLACSRAVIATKTSELEEAKKAMKRNRNLPL 180

Query: 181 PEKAPPASETAQQQQGSDRAAQIKDGDVVNPEPXXXXXXXXXXXTKKLSNASRKRMQRSR 240
           PEKAPPASETAQQQQGSDRAAQIKDGDVVNPEP           TKKLSNASRKRMQRSR
Sbjct: 181 PEKAPPASETAQQQQGSDRAAQIKDGDVVNPEPAAASDASHAASTKKLSNASRKRMQRSR 240

Query: 241 SLGXXXXXXXXXXXXXXXNVQRRKSMRTPVPQPSEHREDLFEIEDLQLAIXXXXXXXDSK 300
           SLG               NVQRRKSMRTPVPQPSEHREDLFEIEDLQLAI       DSK
Sbjct: 241 SLGPAATTKLAATPKEKENVQRRKSMRTPVPQPSEHREDLFEIEDLQLAIGGGGGGGDSK 300

Query: 301 AGTSDPPEQXXXXXXXXQFPRRSSLGRPIRRATERVASYKEMPVNIKLRRS 351
           AGTSDPPEQ        QFPRRSSLGRPIRRATERVASYKEMPVNIKLRRS
Sbjct: 301 AGTSDPPEQAAVAVAAAQFPRRSSLGRPIRRATERVASYKEMPVNIKLRRS 351



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.311    0.126    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,346,149
Number of extensions: 339343
Number of successful extensions: 1095
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 1095
Number of HSP's successfully gapped: 1
Length of query: 351
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 249
Effective length of database: 11,709,973
Effective search space: 2915783277
Effective search space used: 2915783277
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 156 (64.7 bits)